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Evaluation of the Influence of Sulfur-Fumigated Paeoniae Radix Alba on the Quality of Si Wu Tang by Chromatographic and Chemometric Analysis


Affiliations
1 School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2 University of New South Wales, Sydney, NSW 2031, Australia
 

An accurate and reliable method of high-performance liquid chromatographic fingerprint combining with multi-ingredient determination was developed and validated to evaluate the influence of sulfur-fumigated Paeoniae Radix Alba on the quality and chemical constituents of Si Wu Tang. Multivariate data analysis including hierarchical cluster analysis and principal component analysis, which integrated with high-performance liquid chromatographic fingerprint and multi-ingredient determination, was employed to evaluate SiWu Tang in a more objective and scientific way. Interestingly, in this paper, a total of 37 and 36 peaks were marked as common peaks in ten batches of SiWu Tang containing sun-dried Paeoniae Radix Alba and ten batches of Si Wu Tang containing sulfur-fumigated Paeoniae Radix Alba, respectively, which indicated the changed fingerprint profile of SiWu Tang when containing sulfur-fumigated herb. Furthermore, the results of simultaneous determination for multiple ingredients showed that the contents of albiflorin and paeoniflorin decreased significantly (p < 0.01) and the contents of gallic acid and Z-ligustilide decreased to some extent at the same time when Si Wu Tang contained sulfur-fumigated Paeoniae Radix Alba.Therefore, sulfur-fumigation processing may have great influence on the quality of Chinese herbal prescription.
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  • Evaluation of the Influence of Sulfur-Fumigated Paeoniae Radix Alba on the Quality of Si Wu Tang by Chromatographic and Chemometric Analysis

Abstract Views: 93  |  PDF Views: 0

Authors

Ke Pei
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Hao Cai
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Duan Yu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Feng-Xian Qiao
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Si-Cong Tu
University of New South Wales, Sydney, NSW 2031, Australia
Xiao Liu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Xiao-Li Wang
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Xiao-Qing Song
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Kai-Lei Fan
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Bao-Chang Cai
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

Abstract


An accurate and reliable method of high-performance liquid chromatographic fingerprint combining with multi-ingredient determination was developed and validated to evaluate the influence of sulfur-fumigated Paeoniae Radix Alba on the quality and chemical constituents of Si Wu Tang. Multivariate data analysis including hierarchical cluster analysis and principal component analysis, which integrated with high-performance liquid chromatographic fingerprint and multi-ingredient determination, was employed to evaluate SiWu Tang in a more objective and scientific way. Interestingly, in this paper, a total of 37 and 36 peaks were marked as common peaks in ten batches of SiWu Tang containing sun-dried Paeoniae Radix Alba and ten batches of Si Wu Tang containing sulfur-fumigated Paeoniae Radix Alba, respectively, which indicated the changed fingerprint profile of SiWu Tang when containing sulfur-fumigated herb. Furthermore, the results of simultaneous determination for multiple ingredients showed that the contents of albiflorin and paeoniflorin decreased significantly (p < 0.01) and the contents of gallic acid and Z-ligustilide decreased to some extent at the same time when Si Wu Tang contained sulfur-fumigated Paeoniae Radix Alba.Therefore, sulfur-fumigation processing may have great influence on the quality of Chinese herbal prescription.