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Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence:Comparative Study


Affiliations
1 Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico
2 Universidad Autonoma del Estado de Mexico, 50120 Toluca, MEX, Mexico
3 Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana-Xochimilco, 04960 Mexico, DF, Mexico
 

Polycyclic aromatic hydrocarbons (PAHs) are compounds that can be quantified by fluorescence due to their high quantum yield. Haloalkalitolerant bacteria tolerate wide concentration ranges of NaCl and pH. They are potentially useful in the PAHs bioremediation of saline environments. However, it is known that salinity of the sample affects fluorescence signal regardless of the method. The objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detection limits of the excitation, emission, and synchronous fluorescence methods, during the quantification of the residual anthracene concentration from the following haloalkalitolerant actinomycetes cultures Kocuria rosea, Kocuria palustris, Microbacterium testaceum, and 4 strains of Nocardia farcinica, in order to establish the proper fluorescence method to study the PAHs biodegrading capacity of haloalkalitolerant actinobacteria. The study demonstrated statistical differences among the strains and among the fluorescence methods regarding the anthracene residual concentration. The results showed that excitation and emission fluorescence methods performed very similarly but sensitivity in excitation fluorescence is slightly higher. Synchronous fluorescence using Δλ = 150nm is not the most convenient method. Therefore we propose the excitation fluorescence as the fluorescence method to be used in the study of the PAHs biodegrading capacity of haloalkalitolerant actinomycetes.
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  • Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence:Comparative Study

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Authors

Reyna del Carmen Lara-Severino
Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico
Miguel Angel Camacho-Lopez
Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico
Jessica Marlene Garcia-Macedo
Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico
Leobardo M. Gomez-Olivan
Universidad Autonoma del Estado de Mexico, 50120 Toluca, MEX, Mexico
Angel H. Sandoval-Trujillo
Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana-Xochimilco, 04960 Mexico, DF, Mexico
Keila Isaac-Olive
Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico
Ninfa Ramirez-Duran
Universidad Autonoma del Estado de Mexico, 50180 Toluca, MEX, Mexico

Abstract


Polycyclic aromatic hydrocarbons (PAHs) are compounds that can be quantified by fluorescence due to their high quantum yield. Haloalkalitolerant bacteria tolerate wide concentration ranges of NaCl and pH. They are potentially useful in the PAHs bioremediation of saline environments. However, it is known that salinity of the sample affects fluorescence signal regardless of the method. The objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detection limits of the excitation, emission, and synchronous fluorescence methods, during the quantification of the residual anthracene concentration from the following haloalkalitolerant actinomycetes cultures Kocuria rosea, Kocuria palustris, Microbacterium testaceum, and 4 strains of Nocardia farcinica, in order to establish the proper fluorescence method to study the PAHs biodegrading capacity of haloalkalitolerant actinobacteria. The study demonstrated statistical differences among the strains and among the fluorescence methods regarding the anthracene residual concentration. The results showed that excitation and emission fluorescence methods performed very similarly but sensitivity in excitation fluorescence is slightly higher. Synchronous fluorescence using Δλ = 150nm is not the most convenient method. Therefore we propose the excitation fluorescence as the fluorescence method to be used in the study of the PAHs biodegrading capacity of haloalkalitolerant actinomycetes.