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Genetic Characterization of Dahlem Red Layers by PCR-RAPD


Affiliations
1 ILFC, C.V.Sc., Korutla, India
2 C.V.Sc., Korutla, India
3 Project Directorate on Poultry, Rajendranajar, Hyderabad, India
     

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A total of one hundred Randomly Amplified Polymorphic DNA (RAPD) markers were utilized to detect the DNA polymorphisms in a total of 48 full sib mated, half sib mated and non-in bred groups of Dahlem Red birds. Twenty one per cent of the primers tested yielded distinct polymorphic RAPD profiles. Out of 341 amplified bands 204 bands (59.82 %) were found to be polymorphic. The genetic similarity ranged from 83.94 to 87.90 % and, 81.14 to 85.25 % with in group based on band sharing and frequency respectively. Full sib group showed higher genetic similarity then non-in bred group. The genetic similarity based on band frequency ranged from 88.07 to 90.18 %, based on band frequency varied from 72.78 to 79.53 percent. The full sib and half sib groups showed maximum genetic similarity. The genetic distance between full sib and non-inbred groups was found to be the maximum (0.3176), while it was minimum between full sib and half sib groups.

Keywords

Dahlem Red, Inbred, Non-Inbred, RAPD-PCR.
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  • Genetic Characterization of Dahlem Red Layers by PCR-RAPD

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Authors

Ch. Shivaprasad
ILFC, C.V.Sc., Korutla, India
B. Ramesh Gupta
C.V.Sc., Korutla, India
R. N. Chatterjee
Project Directorate on Poultry, Rajendranajar, Hyderabad, India

Abstract


A total of one hundred Randomly Amplified Polymorphic DNA (RAPD) markers were utilized to detect the DNA polymorphisms in a total of 48 full sib mated, half sib mated and non-in bred groups of Dahlem Red birds. Twenty one per cent of the primers tested yielded distinct polymorphic RAPD profiles. Out of 341 amplified bands 204 bands (59.82 %) were found to be polymorphic. The genetic similarity ranged from 83.94 to 87.90 % and, 81.14 to 85.25 % with in group based on band sharing and frequency respectively. Full sib group showed higher genetic similarity then non-in bred group. The genetic similarity based on band frequency ranged from 88.07 to 90.18 %, based on band frequency varied from 72.78 to 79.53 percent. The full sib and half sib groups showed maximum genetic similarity. The genetic distance between full sib and non-inbred groups was found to be the maximum (0.3176), while it was minimum between full sib and half sib groups.

Keywords


Dahlem Red, Inbred, Non-Inbred, RAPD-PCR.