Indian Journal of Science and Technology

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Antibacterial activity of methanol, hexane and petroleum ether extracts of Polyclinum madrasensis was evaluated by cup plate method against various clinical isolates. Two different concentrations (1&4 mg/ml) were analyzed. Methanolic extract of P. madrasensis demonstrated high degree of activity against all tested bacterial isolates in increasing concentrations, whereas hexane extract showed good activity against gram negative pathogens in high concentration and moderate against gram positive pathogens in lower concentration. All strains were resistant to Pet ether extract in both the concentrations when compared to the standard (ciprofloxacin) used. The MIC and MBC for methanolic extract tested in study inferred that the values range between 0.70-0.95 mg/ml and 0.85-1.1 mg/ml respectively. It was concluded from the study that methanolic extract of P. madrasensis exhibits potential antibacterial property.

Keywords

Antimicrobial Activity, Polyclinum Madrasensis, Cup Plate Method, Clinical Isolates
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