Analysis of Nuclei Detection with Stain Normalization in Histopathology Images

This paper proposes a unique detection technique to identify nuclei from microscopy images of breast histopathology slides. The method involves stain normalization, color decomposition and hysteresis thresholding. A comparative analysis of different pre-processed images on nuclei detection reveals an accuracy of 97% for Blue Ratio (BR) images. The technique uses three color channels for accurate detection of nuclei. An analysis of two stain normalization procedure is also presented.