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Toxicology International (Formerly Indian Journal of Toxicology), Vol 19, No 1 (2012), Pagination: 31-34
Abstract
Herbal and natural products have been used in folk medicine for centuries throughout the world. There has been renewed interest in screening higher plants for novel biologically active compounds, particularly those that effectively intervene in human ailments in the field of chronic diseases. The present study has been taken up to evaluate the free radical scavenging activity and tumor cell suppression potential of Premna serratifolia leaf in various in vitro model systems. The methanolic extract of P. serratifolia leaf was obtained by soxhlet extraction method. The superoxide radical scavenging activity, nitric oxide radical, hydroxyl radical, DPPH radical and ABTS radical scavenging activity and lipid peroxidation were determined. The tumor cell suppression cell potential was determined in three different cancer cell lines MCF7 (breast cancer), HepG2 (liver cancer) and A549 (lung cancer) by SRB assay. The study showed that the methanolic extract of P. serratifolia was having free radical scavenging activity against superoxide radical, nitric oxide radical, hydroxyl radical, DPPH radical, ABTS radical and inhibition of lipid peroxidation. The IC50 value showed the efficacy was dose dependent. The test extract showed cytotoxic activity against MCF7, HepG2 and A549 cells. The GI50, TGI and LC50 values were determined against each cell line and compared with standard drug Adriamycin. The present study proved the free radical scavenging activity and tumor cell suppression potential of P. serratifolia leaf in the selective in vitro model systems. The further study has to be carried out in the aspects of isolation of functional molecules of the extract.
Keywords
Antioxidants, cytotoxicity, tumor, Premna serratifolia, tumor cell
