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Kalyanaraman, V.
- Evolutionary Gene Expression of Lactate Dehydrogenase in Water Snakes Cerberus rhynchops and Xenochrophis piscator
Authors
1 School of Enzymology and Environmental Toxicology (SEET) PG & Research Dept. of Zoology, Sir Theagaraya College, Chennai, Tamilnadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 4 (2010), Pagination: 422-424Abstract
Species differences are known to exist with reference to LDH profile. A four-band isoenzyme pattern is a feature in most snakes, which is a deviation from the primitive ancestor. The LDH morphic state of two water snakes, Cerberus rhynchops and Xenochrophis piscator belonging to the same family Colubridae was selected for the geneological understanding since one of the species is a known salt tolerant. The study was undertaken to observe the fractions of the key isoenzyme LDH subunit diversity in an evolutionary backdrop.Keywords
Lactate Dehydrogenase, Cerberus rhynchops, Xenochrophis piscatorReferences
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- Acute Toxicity of some Agriculture Fertilizers to Fingerlings of Catla Catla
Authors
1 School of Enzymology and Environmental Toxicology, Sir Theagaraya College, Chennai-600 021, IN
Source
Indian Journal of Science and Technology, Vol 4, No 7 (2011), Pagination: 770-772Abstract
Nitrogen fertilizers tend to increase ammonium concentration especially in the aquatic medium. To evaluate its impact, the acute toxicity of ammonium sulphate, urea and a composite fertilizer (15:15:15) (NPK-1) was assessed in fingerlings of fresh water fish Catla catla using a static test system. The toxicity of ammonium sulphate, urea and NPK1 to C. catla increased with increasing fertilizer concentration and duration of exposure. The concentration that killed 10% (LC10) and 90% (LC90) of fish varied with the fertilizers. The concentrations of ammonium sulphate, urea and NPK- 1 that killed 50% of fingerlings of C. catla within 96-h (96-h LC50) were 0.12, 0.19 and 0.18 g/L, respectively. Ammonium sulphate concentrations killing 50% (LC50) of the fingerlings of fresh water fish Catla catlawere 0.23, 0.17, and 0.14, 0.12 g/L during 24hr, 48hr, 72hr and 96hr correspondingly. Ammonium sulphate was found to be more toxic to the juvenile fresh water fish C. catla compared to urea and NPK-1.Keywords
Acute Toxicity, Nitrogen Fertilizers, Catla catla, Fish, PollutionReferences
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- Effect of Lead on the Expression of Nutritional Content in Edible Lobster, Thenus Orientalis (Lund, 1793)
Authors
1 School of Enzymol. & Environ. Toxicol.,,P.G. & Res. Dept. of Zoology, Sir Theagaraya College, Chennai, IN
Source
Indian Journal of Science and Technology, Vol 2, No 10 (2009), Pagination: 17-22Abstract
The edible lobster, Thenus orientalis is an important food of commercial interest in the parts of South India. Evaluation of toxic effect of lead on the chosen animal for the LC50 value and effect of heavy metal lead on the nutritional status viz. protein, carbohydrate and lipid in ovary, spermatheca, hepatopancreas, muscle and haemolymph was made. The results assume greater interest as most water bodies are increasingly subjected to environmental pressure due to pollution.Keywords
Lead Toxicity, Lobster, LC50, Protein, PollutionReferences
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- In Vitro Cytotoxicity Studies of Cerium Oxide Nanoparticles
Authors
1 Department of Zoology, S.I.V.E.T College, Chennai-600073, IN
2 GLR Laboratories Pvt Ltd, MK7 8HG, GB
3 GLR Laboratories Pvt Ltd, Chennai-600060, IN
Source
Indian Journal of NanoScience, Vol 4, No 1 (2016), Pagination: 1-5Abstract
Background/Objectives: Cerium oxide nanoparticles (CeO2-NP), a member of the lanthanide series of rare metal oxide, have beneficial property like radicals scavenging and being examined as a potent candidate for many biomedical applications. The aim of the study is to investigate certain key in vitro cytotoxicity endpoints using CeO2- NP.
Methods/Statistical analysis: The techniques applied include, characterization, MTT assay, LDH release, ATP measurement and Caspase 3/7 activity assay. The results were subjected to null hypothesis testing to determine its significance.
Findings: CeO2-NPshowed mild cytotoxicity with a loss of MTT reactivity and depletion in ATP production. CeO2-NP treatment also resulted in an increase in Caspase3/7 activity and leakage of LDH in the tested cell line suggesting that it induces apoptosis via release of reactive oxygen species or through the physical damage of the cell membrane.
Improvements/Applications: Thefindings add base line information to the biocompatibility and toxicity data sheet on CeO2-NP. The results will also be beneficial in risk assessing CeO2-NP based medical devices.
Keywords
Cerium Oxide Nanoparticles, Cytotoxicity, L-929 Cells.References
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