A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Roy, Sohini
- Extraction of Chitosan and its Oligomers from Shrimp Shell Waste, their Characterization and Antimicrobial Effect
Authors
1 Department of Animal Nutrition, ICAR-National Dairy Research Institute, Karnal, Haryana, IN
2 Department of Animal Nutrition, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru, Karnataka, IN
3 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, IN
Source
Veterinary World, Vol 10, No 2 (2017), Pagination: 170-175Abstract
Aim: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid.
Materials and Methods: Chitosan was extracted from the shrimp shell waste by the chemical method and characterized by FT-IR. Chitooligomers were produced by depolymerising chitosan using nitrous acid, and the chitooligomers were tested for antimicrobial effect against four gut pathogenic organisms, i.e., Enterobacter aerogen (National Collection of Dairy Culture [NCDC] 106), Enterococcus faecalis (NCDC 119), Escherichia coli (NCDC 134), and Staphylococcus aureus (NCDC 109) by well diffusion method using Muller-Hinton agar. A pure culture of pathogenic organisms was collected from NCDC, ICAR-National Dairy Research Institute, Karnal.
Results: Extracted chitosan characterized by FT-IR and chitooligomers demonstrated antimicrobial effect against four gut pathogenic organisms used in this study. Zone of inhibitions (mm) were observed in E. faecalis (13±0.20), E. coli (11.5±0.4), S. aureus (10.7±0.2), and E. aerogen (10.7±0.3). E. faecalis showed larger inhibition zone as compared to all other organisms and inhibitions zones of E. aerogen and S. aureus were comparable to each other.
Conclusion: Shrimp waste can be utilized for chitosan production, and the chitooligomers can be used as feed additive for gut health enhancement and have potential to replace antibiotics from the feed. Along with value addition pollutant load could be reduced by waste utilization.
Keywords
Chitin, Chitooligomers, Chitosan, Fourier Transform Infrared, Shrimp Waste.- Xylooligosaccharides Production from Tobacco Stalk Xylan using Edible Acid
Authors
1 ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru 560 030, IN
Source
Current Science, Vol 117, No 9 (2019), Pagination: 1521-1525Abstract
In the present study, a process was developed to hydrolyse xylan from tobacco stalks into xylooligosaccharides (XOS) by applying tartaric acid, an edible acid. The tobacco stalks contained approximately hemicelluloses (16.99%), cellulose (50.8%) and lignin (15.6%). Use of 8% KOH or NaOH under overnight incubation resulted in almost complete recovery of xylan from the tobacco stalks. Application of 1 M tartaric acid at 90°C hydrolysed xylan into XOS and the highest (0.357 mg/ml) yield of XOS was recorded after 120 minutes. Hence, prebiotic XOS could be produced from the tobacco stalk xylan by applying tartaric acid.Keywords
Edible Acid, Tobacco Stalk, Xylan, Xylooligosaccharides.References
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- In Vitro Study on the Protective Effect of Centella asiatica< (Thankuni) in Fish Roe Induced Hyperuricaemic Rats
Authors
1 Department of Food & Nutrition, Vidyasagar College, Kolkata, West Bengal, IN
2 Department of Food & Nutrition, Women’s College, Calcutta, Kolkata, West Bengal, IN
3 Department of Home Science, University of Calcutta, Kolkata, West Bengal, IN
Source
Indian Science Cruiser, Vol 34, No 4 (2020), Pagination: 51-57Abstract
Hyperuricaemia is a disorder of purine metabolism which is frequently encountered in the clinical setting. The current in vitro study aims at finding out the effect of dietary purine intake on serum uric acid levels and the efficacy of Centella asiatica for treating this condition. Male Wistar rats of Group C, P and PT received stock diet, stock diet+fish roe (5g/day), stock diet+fish roe (5g/day)+Centella asiatica (150mg/kg) paste respectively. In PT group, serum uric acid decreased as compared to P, and it was comparable to C group. The herb also showed positive effect on liver function tests. It can be concluded that Centella asiatica can act as a potent herbal remedy in hyperuricaemia as well as gout.Keywords
Hyperuricaemia, Gout, Liver Function, Herbal Remedy.References
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