Abstract Views :151 |
PDF Views:0
Authors
Affiliations
1 Department of Zoology, Ranchi University, Ranchi-834 008, Jharkhand, IN
Source
Nature Environment and Pollution Technology, Vol 11, No 1 (2012), Pagination: 99-104
Abstract
The experiment was conducted to study the bacterial population in fly ash amended soil with and without earthworm inoculation in laboratory by dilution plate method. It was found that in presence of Drawida willsi at 5% fly ash (FA) amendment showed maximum population of 43.2 ± 1.15 × 109 cfu/ g soil from initial population. At 10% and 15% FA amendment gradual decline to 14.3 ± 0.4 × 109 and 7.63 ± 0.6 × 109 cfu/g soil from 21.7 ± 0.36 × 109 and 15.33 ± 1.1 × 109 cfu/g soil over 90 days of experiment at an interval of 15 days was observed. In absence of earthworms gradual decline in population was seen in the three concentrations of fly ash amendment to 22.7 ± 0.5 × 109, 11.6 ± 0.7 × 109 and 3.43 ± 0.7 × 109 cfu/g soil respectively. ANOVA test showed that with earthworms the effect of concentration of FA on the bacterial population was significant (F = 69.9, df = 6, 2, p≤0.05) and in the absence of earthworm both concentration and time interval played a significant role (F = 155.1, df = 6, 2, p≤0.1; F= 10.5, df = 6, 2, p ≤ 0.001). Lower dose of 5% fly ash in soil proved to be optimum for the bacterial activity in the presence of earthworms. Morphological details of different bacterial colonies were assessed and found to be of punctiform, irregular, circular and filamentous shapes with punctiform dominating the culture.
Keywords
Fly Ash Amended Soil, Earthworm, Drawida willsi, Bacterial Population.
Full Text
