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Soeharto, Setyawati
- In Vitro Study of Bronchial Relaxation Mechanism of Coptosapelta Flavescens Korth Root’s Methanol Extract On Receptors
Authors
1 Doctoral Program of Medical Sciences, Brawijaya University, Malang, ID
2 Department of Pharmacology, Brawijaya University, Malang, ID
3 Department of Microbiology, Brawijaya University, Malang, ID
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1770-1776Abstract
Objective: To evaluate the bronchorelaxation effect of methanol extract of Coptosapelta flavescens Korth. ischolar_main (MECFR) on cholinergic, histamine receptors and beta2-adrenoceptors, in vitro.
Method: The research design was experimental in which a 4-mm long guinea pig’s bronchus was kept in an isolated organ bath containing Kreb’s-Henseleit solution at 37°C, pH 7.4 and flooded with carbogen gas. The study was divided into 3 groups: in Group 1 and 2, the bronchial rings were incubated in 3 concentrations of MECFR, and then contracted with cumulative doses of methacholine and histamine, respectively; in Group 3, the bronchial ring was incubated in 3 concentrations of propranolol, contracted with a single dose of methacholine, and then the MECFR was administered in cumulative doses.
Results: In both Group 1 and Group 2, the dose-response curves of all 3 doses of MECFR shifted to the right with lower Emax (P<0.05) as compared to the negative control. In Group 3, the dose-response curves showed MECFR has bronchorelaxation effect on bronchial rings both in the presence of propranolol andwithout, with similar Emax (P>0.05).
Conclusion: MECFR exhibits bronchorelaxation effects by acting as non-competitive antagonist at the cholinergic and histamine1 receptors, and also by acting as beta2-adrenoceptor agonist.
Keywords
Beta2-adrenoceptor, Cholinergicreceptor, Coptosapelta Flavescens Korth Root, Histamine Receptor.
- Modification of the Vancouver Scar Scale (Vss) Score for Scarring Assessment Using Rattus Novergicus Abnormal Scar Model
Authors
1 Doctoral Program, Faculty of Medicine, Brawijaya University, Malang 65145,, ID
2 Pharmacology Department, Faculty of Medicine, Brawijaya University, Malang 65145, ID
3 Clinical Pathology Departement, Faculty of Medicine, Brawijaya University, Malang 65145,, ID
4 Faculty of Medicine and Health Sciences, State Islamic University of Malang, Malang 65144, ID
Source
Research Journal of Pharmacy and Technology, Vol 15, No 3 (2022), Pagination: 1313-1318Abstract
The abnormal scar is a unique fibrosis disease because it only occurs in humans (Homo sapiens). Researchers now challenge no other animal species, including primates, are found to naturally form scar7, whereas animal models are essential references for human treatment modalities. This study aimed to determine the Modified Vancouver Scar Scale (VSS) score with the addition of collagen density parameters used to assess the scar in experimental animals quantitatively and generates a better assessment of the scar. The cross-sectional analytical survey method was adopted. The experimental animal was Rattus novergicus. The Modified VSS score was applied to assess the normal and abnormal scar data. The Likert categorization guidelines were used to obtain the VSS Score modification score. The collagen density and VSS had a significantly different based on T-test (p <0.05). The differences were also shown by the control and treatment groups. On the correlation analysis, there were the positive coefficient (0.722). The result can be described that the collagen density increases, when the VSS score is high. It also showed the differences based on the score (p <0.05). The Modified VSS score calculation's final results are classified into three groups namely Good (ranged from 0 to 1); Medium (ranged from 2 to 4); and Adverse (ranged from 5 to 6). The Modified VSS score is possibly to be used for the scar assessment to the Rattus novergicus abnormal scar model.
Keywords
Abnormal scar, Collagen density, Rattus novergicus, VSS score.References
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