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Gabhe, Satish Y.
- Comparative Antioxidant Activity of Three Garlic Varieties:An in vitro Study
Authors
1 C. U. Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (West), Mumbai-400049, IN
2 C. U. Shah College of Pharmacy, S.N.D.T Women’s University, Mumbai-400 049, IN
3 ICMR Advanced Centre of Reverse Pharmacology in Traditional Medicine, Kasturba Health Society, Vile Parle (West), Mumbai- 400 056, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 3, No 4 (2011), Pagination: 162-165Abstract
The present paper discuss the antioxidant activity of methanol extracts of commonly available three garlic varieties in India using various in vitro methods Viz, DPPH assay, total reducing power and TBARS assay. The results were analyzed statistically by regression method and IC50 values were calculated for all the extracts. The amounts of total phenolics and flavonoids were also determined for each extract of garlic. All the extracts exhibited good free radical scavenging activity. Antilipid peroxidation activity of all garlic varieties was comparable to standard antioxidant, ascorbic acid. Methanol extract of single bulb variety of garlic exhibited better antioxidant activity among all three garlic varieties. The study provides evidence for the beneficial role of garlic. The antioxidant activity of garlic extracts will effectively contribute to the prevention of various chronic conditions and also confirms its ancient traditional claims.
Keywords
Garlic, Allium sativum, Antioxidant, Free Radical Scavenging.- Isolation and Quantitative Analysis of a Bioactive Polyphenol - Catechin in Anacardium occidentale Linn. (Leaves and Testa) by HPLC Analysis
Authors
1 C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Mumbai-400049, IN
2 C. U. Shah College of Pharmacy, S.N.D.T. Women's University, Juhu Campus, Juhu Tara Road, Santacruz West, Mumbai-400 049, M.S., IN
3 ICMR Advanced Centre of Reverse Pharmacology in Traditional Medicine, Kasturba, Health Society, Vile Parle-(W), Mumbai-400 056, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 2, No 5 (2010), Pagination: 372-376Abstract
The present paper reports a method developed for isolation, characterization, structural elucidation and quantitative analysis of bioactive polyphenol - Catechin, from extracts of leaves and testa of Anacardium occidentale Linn (Cashew). Isolation of Catechin was carried out by Preparative TLC. The isolated Catechin was characterized by P-NMR, MS, IR spectroscopy and various chemical and chromatographic analysis. The method was established by using HPLC analysis and quantitation of isolated Catechin with marker Catechin. The purity of isolated Catechin was found to be 99.30% based upon the comparison of peak purity and peak areas of isolated Catechin and marker Catechin.
The HPLC separation system consisted of a C18 reversed-phase column, an isocratic elution system of methanol-water (9:1 v/v), and a UV-Visible detector with 254nm as the detection wavelength. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.2 μg and 0.6 μg respectively with an Rt value of 2.5 min.
The maximum amount of Catechin was found in aqueous extract of leaves (5.70%) and testa (13.65%).
Thus the developed HPLC method is rapid and simple technique for separation and determination of Catechin from extracts of A.occidentale leaves and testa. The method can be suitably applied for determination of Catechin from various plant extracts.
Keywords
Catechin, Anacardium occidentale Linn., Polyphenol, HPLC.- Spectrophotometric and Spectrofluorimetric Methods for Simultaneous Estimation of Amlodipine and Metformin in Bulk and Synthetic Mixture
Authors
1 Department of Pharmaceutical Sciences, Jaipur National University, Jaipur - 302 025, IN
2 C. U. Shah College of Pharmacy, S.N.D.T. Women’s University, Mumbai - 400 049, IN
3 Smt. R. B. Patel Mahila Pharmacy College, Atkot - 360 040, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 4 (2011), Pagination: 640-646Abstract
Purpose: the purpose of this article is to develop two simple, rapid, accurate and economical spectrophotometric methods and a sensitive spectrofluorimetric method for the simultaneous estimation of amlodipine (AD) and metformin (MT) in bulk and synthetic mixture.
Method: The Spectrophotometric determination involved simultaneous equations method that measured the absorbances of both the drugs at 238 nm (λmax of metformin) and at 361 nm ( λmaxof amlodipine) and Q- absorbance ratio method that measured the absorbance of both the drugs at 238 nm (λmax of metformin) and at isoabsorptive point (256 nm). A spectrofluorimetric determination is based on the reaction between the drugs and 1- dimethylaminonaphthalene-5-sulphonyl chloride (Dansyl chloride) in presence of 1 M sodium carbonate (pH 10) to yield a highly fluorescent derivative that is measured at 450 nm and 520 nm after excitation at 345 nm for AD and MT, respectively. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized.
Results and discussions: Both spectrophotometry methods showed linearity over the range of 5-90 μg ml-1 for AD and 1-10 μg ml-1 for MT. The fluorescence intensity were linear over the range of 0.1-3.5 μg ml-1 for AD and 0.05-1.3 μg ml-1 for MT. Results of analyses were validated statistically and through recovery studies.