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The attachment, penetration, infectivity and multiplication of eight native isolates of Entomopathogenic nematodes (EPNs), Heterorhabditis sp. (IISR-EPN 01), Steinernema sp. (IISR-EPN 02), Steinernema sp. (IISR-EPN 03), S. carpocapsae (IISR-EPN 06), Oscheius spp. (IISR-EPN 04, 05, and 08) and O. gingeri were tested against larvae of Conogethes punctiferalis at different temperatures viz., 20, 25, 30 and 35°C. The temperature significantly affected attachment, penetration, pathogenicity and multiplication ability of infective juveniles (IJs) of all tested EPNs. Among the test temperatures, maximum mortality of larvae was found at 30°C followed by 25°C, whereas the least mortality was recorded at 20 and 35°C. Maximum number of infective juveniles was multiplied at 30°C, however minimum multiplication was recorded at 35°C. Among the test EPNs, no multiplication of Heterorhabditis sp. (IISR-EPN 01), Steinernema sp. (IISR-EPN 03) and O. gingeri was recorded at 20°C. IJs attached to larvae of C. punctiferalis in higher number after 6 h at 25 and 30°C. Whereas, maximum number of IJs penetrated into C. punctiferalis larvae at 30°C. Therefore, the optimal temperature for infection and development for all promising EPNs was 30°C.

Keywords

Entomopathogenic Nematodes, Ginger, Shoot Borer, Temperature.

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References

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Eco-Friendly Management of Lepidopteran Insect Pests through Entomopathogenic Nematodes

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Authors

Rashid Pervez ¹, Uma Rao ¹

Affiliations
1 Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, IN

Source

Journal of Biological Control, Vol 34, No 1 (2020), Pagination: 66-72

Abstract

In a recent survey, we have isolated six entomopathogenic nematodes from Hapur, Shamli, Saharanpur and Meerut districts of western Uttar Pradesh. Preliminary identification at genus level classified one isolate as Heterorhabditis sp., two isolates as Steinernema spp. and three as Oscheius spp. These six newly isolated EPNs have been designated as Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02), Steinernema sp. (IARI-EPN RP 03), Oscheius sp. (IARI-EPN RP 04), Steinernema sp. (IARI-EPN RP 05) and Heterorhabditis sp. (IARI-EPN RP 06). All these were evaluated for infectivity against lepidopteran larvae, Maruca vitrata infesting pigeonpea, Pieris brassicae infesting mustard and Spodoptera litura infesting chickpea. Among the tested EPNs, Steinernema sp. (IARI-EPN RP 03), Oscheius sp. (IARIEPN RP 04), Heterorhabditis sp. (IARI-EPN RP 06) were found to be promising against M. vitrata as they showed 100% mortality within 48 h followed by Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02) which took 72 h, while Steinernema sp. (IARI-EPN RP 05) took about 120 h to kill the insects. Oscheius sp. (IARI-EPN RP 02), Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN RP 06) gave 100% mortality of P. braissicae within 48 h followed by Oscheius sp. (IARI-EPN 04) and Steinernema sp. (IARI-EPN RP 05) that killed larvae after 72 h, while Oscheius sp. (IARI-EPN RP 01) took about 120 h. Lastly, Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN 06) were found promising for S. litura as both of them caused 100% mortality within 48 h followed by Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02) and Steinernema sp. (IARI-EPN RP 05) that took 72 h, while, Oscheius sp. (IARI-EPN RP 04) was effective at about 120 h. The present results indicated that both Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN RP 06) were highly virulent against the tested lepidopteran insect pests. Further evaluation of these new EPN isolates under field conditions will indicate their utility in integrated pest management.

Keywords

Biological Control, Entomopathogenic Nematodes, Insects, Lepidoptera.

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Pervez, Rashid

Effect of Temperature on the Infectivity of Entomopathogenic Nematodes against Shoot Borer (Conogethes punctiferalis Guen.) Infesting Ginger (Zingiber officinale Rosc.)

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Eco-Friendly Management of Lepidopteran Insect Pests through Entomopathogenic Nematodes

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