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Hadi, Priyo
- Expression of FGF-2 and Fibronectin in Citrus limon Fruit Peel Malang Essential Oil Gel Treated Traumatic Ulcer in Diabetic Wistar Rats (Rattus novergicus)
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Authors
Raziv Ganesha
1,
Iwan Hernawan
1,
Hening Tuti Hendarti
1,
Desiana Radithia
1,
Priyo Hadi
1,
Nurina Febriyanti Ayuningtyas
1,
Diah Savitri Ernawati
1
Affiliations
1 Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, ID
1 Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3350-3354Abstract
Diabetes Mellitus is a metabolic disease that delayed traumatic ulcer healing process. Citrus limon contains fumaric acid, d-limonene and citral that can accelerate the healing of traumatic ulcer. The aims of this study were to investigate the effect of 0.78% Citrus limon essential oil topical application can increase Fibroblast Growth Factor-2 (FGF-2) and Fibronectin expression. This study was true experimental analytic study with random sampling methods. The study was conducted on 30 male Wistar rats (Rattus novergicus) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa. These were divided into six groups; three each for control and treatment groups. Each control and treatment group consisted of five rats. The control groups treated with CMC 5% gel and treatment groups were administered with Citrus limon peel essential oil gel. The expression of FGF-2 and Fibronectin was observed on Days 5, 7 and 9. Furthermore, mice sacrificed and the lower lip labial mucosa tissue of mice has been taken to make the Histopathology Anatomy preparation by means of immunohistochemical examination with monoclonal antibodies anti-FGF2 and anti-Fibronectin. ANOVA analysis was performed to analyze the difference between group (p<0.05). The increased FGF-2 and Fibronectin expression in the treatment group as compared to that of the control group. There were significant differences (p<0.05) of both FGF2 and Fibronectin expression between the two groups. The citrus limon peel essential oil gel increased the FGF-2 and Fibronectin expression during the healing process of traumatic ulcers on the oral mucosa of diabetes afflicted Wistar rats (Rattus novergicus).Keywords
Diabetes Mellitus, Citrus limon Essential Oil Gel, Traumatic ulcer, Fibroblast Growth Factor-2, Fibronectin, Herbal Medicine.
References
- Nwauche KT, Monago CC, Frank O. Management of Diabetes Mellitus with Combined Therapy of Reducdyn and Metformin in Streptozotocin-induced Diabetic Rats. Research J. Pharm. and Tech. 2014, 7(1):39-43.
- Kamtekar S, Keer V. Management of Diabetes: A Review. Research J. Pharm. and Tech. 2014;7(9):1065-1072.
- Kumari MS, Babu MK, Sulthana R, Srinivas M, Prasanthi C. Diabetes Mellitus: Present status and Drug therapy Updates. Research J. Pharm. and Tech. 2014;7(1):84-94.
- Rupashri SV, Gheena S. Recent Advances in Diabetes Research. Research J. Pharm. and Tech. 2016; 9(10):1806-1808.
- Departemen Kesehatan Republik Indonesia. National Basic Health Research Report 2013. 83-97.
- Glick, M. Burket’s Oral Medicine 12th Ed. People Medical Publishing House, Connecticut-USA, 2013;87-88.
- Chaiprateep E, Khobjai W, Noysang N. Clinacanthus nutans-Based Mucoadhesive Films for Oral Ulcers. Research J. Pharm. and Tech 2018; 11(11): 5089-5095.
- Regezi, J., Sciubba, J. and Jordan, R. Oral Pathology: Clinical Pathologic Correlation.2012; 22-25.
- Larjava, H. Oral Wound Healing: Cell Biology And Clinical Management, Pondicherry: John Wiley and Sons, 2012;49, 186-188.
- Gupta N, Jain UK. An Update- Prominent Wound Healing Property of Indigenous Medicines-: A Review. Research J. Pharm. and Tech. 2011;4(2):203-213.
- Guo, S. and DiPietrio, L. Factors Affecting Wound Healing. Journal of dental research, 2010;89(3): 219–229.
- Majumdar M Samanta A, Roy A. Study of wound healing activity of different formulations of Nigella sativa seed extract. Research J. Pharm. and Tech. 2016; 9(12):2097-2105.
- Singh D, Daharwal SJ, Rawat M. Hydrogels- A Potent Carter in Wound Healing. Research J. Pharm. and Tech. 2008;1(1): 6-13.
- Misra PM, Ravichandiran V, Aanandhi MV. Design, Synthesis and In Silico Molecular Docking Study of N-carbamoyl-6-oxo-1-phenyl-1, 6-dihydropyridine-3-carboxamide derivatives as Fibroblast growth factor 1 inhibitor. Research J. Pharm. and Tech. 2017; 10(8): 2527-2534.
- Schultz, G.S. and Wysocki, A. Interaction between extracellular matrix and growth factors in wound healing. Wound Rep Reg, 2008;17: 134-147.
- Yun, Y.R., Won, J.E., Jeon, E., Lee, S., Kang, W., Jo, H., Jang, J.H., Shin, U.S., and Kim, H.W. Fibroblas growth factors: biology, function and application for tissue regeneration. J. Tiss Engineer, 2010;1-18.
- Al-wailli, N., Hozzein, W.N., Badr, G., Al-Ghamdi, A., Al-Waili, H., Salom, K., and Al-Waili, T. Propolis and bee venom in Diabetic Wounds; A Potential Approach That Warrant Clinical Investigation. Afr J Tradit Complement Altern Med, 2015;12(6):1-11.
- Puspasari A, Harijanti K, Soebadi B, Hendarti HT, Radithia D, Ernawati DS. Effects of topical application of propolis extract on fibroblast growth factor-2 and fibroblast expression in the traumatic ulcers of diabetic Rattus norvegicus. J Oral Maxillofac Pathol. 2018 Jan-Apr;22(1):54-58. doi: 10.4103/jomfp.JOMFP_82_17.
- Ernawati DS, Puspa A. Expression of vascular endothelial growth factor and matrix metalloproteinase-9 in Apis mellifera Lawang propolis extract gel-treated traumatic ulcers in diabetic rats. Vet World. 2018 Mar;11(3):304-309. doi: 10.14202/vetworld.2018.304-309. Epub 2018 Mar 14.
- Haidari F, Mansoori E, Zakerkish M, Haghighizadeh M. The Relationship between Metabolic Factors and Quality of Life Aspects in Type 2 Diabetes Patients. Research J. Pharm. and Tech. 2017; 10(5): 1491-1496.
- Le N, Rose M, Levinson H, Klitzman B. Implant Healing in Experimental Animal Models of Diabetes. Journal of Diabetes Science and Technology. 2011;5(3): 605-618.
- Ciobotaru E. Development of Improved Animals Models for the Study of Diabetes. INTECH open sciences. 2013;1-5.
- Erwin, E., Etriawati, E., Muttaqien, M., Pangestiningsih, T.W., dan Widyarini S. The expression of insulin in the mice pancreas (Mus musculus) induced with repeated streptozotocin. Jurnal Kedokteran Hewan, 2013;7(2): 97-100.
- King, A.J.F. The Use of Animal Models in Diabetes Research. British Journal of Pharmacology, 2012;166(3):877–894.
- Soelistijo SA, Novida H, Rudijanto A, Pradana S, Ketut S, Asman M, Harsinen S, Dharma L, Alwi S, Bowo P, Yuanita AL, Dyah P, Nanny NS, Made RS, Made PD, Agus Y, Laksmi S, Sugiarto KW, Sucipto S, Hendra Z. Konsensus Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia. PB PERKENI. 2015;11.
- Wang Z, Yang Y, Xiang X, Zhu Y, Men, J, He M. Estimation of the normal range of blood glucose in rats. Wei Sheng Yan Jiu, 2010;9(2): 33–137, 142.
- Arundina I, Yuliati Y, Soesilawati P, Damaiyanti DW, Maharani D. The effects of golden sea cucumber extract (Stichopus hermanii) on the number of lymphocytes during the healing process of traumatic ulcer on wistar rat’s oral mucous. Dental Journal. 2015; 48(2): 100-103.
- Hanum F, Ernawati DS, Jusri M. Effectiveness of Probolinggo blue grape (Vitis vinifera) extract on fibroblasts proliferation in traumatic ulcer of Rattus novergicus albino. Oral Medicine Dental Journal. 2013;4(2): 103-112.
- Larasati AR, Soesilawati P, Arundina I. Effect of Stichopus hermanii extract to increase re-epithelization in healing process of traumatic ulcer in wistar rat’s oral mucous. Oral Biology Journal. 2013; 5(2): 41-46.
- Nielsen E, Ladefoged O, Søborg O. Evaluation of health hazards by exposure to d-limonene and proposal of a health-based quality criterion for ambient air. The Danish Environmental Protection Agency. Copenhagen Denmark. 2013;11-13.
- Amorim JL, Simas DLR, Pinheiro MMG, Moreno DSA, Alviano CS, Ribeiro da Silva AJ, Fernandes PD. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species. PLoS ONE. 2016; 11(4): 1-18.
- Campelo LM, de Almeida AAC, de Freitas RLM, Cerqueira GS, de Sousa GF, Saldanha GB, Feitosa CM, de Freitas RM. Antioxidant and antinociceptive effects of citrus lemon essential oil in mice. Journal of Biomedicine and Biotechnology. 2011;1-8.
- Murali, R., Karthikeyan, A., and Saravanan, R. Protective effects of d-limonene on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rats. Basic dan Clinical Pharmacology dan Toxicology, 2013;112:75–181.
- Kumar, Abbas, Aster. Robbins basic pathology 9th ed, Elsevier Saunders. 2015; (2):55-60.
- Topical Application of Citrus limon Peel Essential Oil Increased Pro-Inflammatory Cytokines Expression on Type IV Hypersensitivity Reaction
Abstract Views :139 |
PDF Views:76
Authors
Fatma Yasmin Mahdani
1,
Ulyasari Rachmaningayu
2,
Elva Anjani
2,
Asri Dewanti Adhi Nugrahaeni
2,
Naqiya Ayunnisa
3,
Salsabila Fitriana Putri
3,
Meircurius Dwi Condro Surboyo
1,
Nurina Febriyanti Ayuningtyas
1,
Priyo Hadi
1,
Hening Tuti Hendarti
1,
Diah Savitri Ernawati
1
Affiliations
1 Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
2 Dental Profession Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
3 Bachelor Dental Science Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
1 Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
2 Dental Profession Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
3 Bachelor Dental Science Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - 60132, ID
Source
International Journal of Medical and Dental Sciences, Vol 12, No 1 (2023), Pagination: 2063-2070Abstract
Introduction: Citrus limon peels essential oil has the potential to be developed as a topical medicine due to its active components. The essential oil has been demonstrated in numerous studies to have anti-inflammatory, anti-microbial, antifungal, and antioxidant properties. A study is required to determine whether Citrus limon peel essential oil can cause a type IV hypersensitivity reaction by examining the expression of the cytokines TNF-, IL-1, and IL-6 as well as the clinical manifestations on an animal skin. In this study, the effect of topically applying the essential oil of Citrus limon peel on the expression of TNF-α, IL-1, and IL-6 as well as on type IV hypersensitivity reaction symptoms was examined. Methods: This study is based on the guinea-pig maximisation test method in NADFC RI Regulation Number 7 of 2014, with two groups (control group and treatment group) for each set of ten samples. Citrus limon peels essential oil was administered to the treatment group, whereas CMC-Na was given to the control group. Animal models were euthanized after which the tissues were prepared as specimens for immunohistochemical staining. The expression of cytokines was computed and examined using a light microscope. Results: There were no clinical signs of type IV hypersensitivity in either group, and the expression of TNF-α, IL-1 and IL-6 in the treatment group was found higher than in the control group. Conclusion: After topical administration of Citrus limon peels essential oil, there was an increase in the expression of TNF-α, IL-1 and IL-6 without any clinical symptoms of type IV hypersensitivity.Keywords
Citrus limon, Essential Oil, IL-6, TNF-α, IL-1, Type IV HypersensitivityFull Text
References
- Kumar G, Jalaluddin Md, Rout P, Mohanty R, Dileep CL. Emerging trends of herbal care in dentistry. Journal of Clinical and Diagnostic Research. 2013; 7(8):1827-9.
- Bustanussalam. Pemanfaataan obat tradisional (herbal) sebagai obat alternatif. BioTrends. 2016; 7(1):20-5.
- Turner T, Burri B. Potential nutritional benefits of current citrus consumption. Agriculture. 2013; 3(1):170-87. https:// doi.org/10.3390/agriculture3010170
- Kostner L, Anzengruber F, Guillod C, Recher M, Grendelmeier PS, Navarini AA. Allergic contact dermatitis. Immunology and Allergy Clinics of North America. 2017; 37(1): 141-52. https://doi.org/10.1016/j.iac.2016.08.014 PMid:27886903
- Dev C, Nidhi SSRR. Basketful benefit of Citrus limon. International Research Journal of Pharmacy. 2016; 7(6):1- 4. https://doi.org/10.7897/2230-8407.07653
- Jain N, Sharma M. Evaluation of citrus lemon essential oil for its chemical and biological properties against fungi causing dermatophytic infection in human beings. Analytical Chemistry Letters. 2017; 7928:402-9. https://doi.org/10.10 80/22297928.2017.1349620
- Mahdani FY, Ernawati DS, Hadi P, Soebadi B, Mardiyana SD, Susanti D, et al. Citrus limon L. gel stimulate lymphocytes and interleukin-10 expression in traumatic diabetic oral ulcers. Phcog Res. 2020; 12:299-302.
- Ben HA, Ben HN, Smaoui S, Hamdi N. Citrus lemon essential oil: Chemical composition, antioxidant and antimicrobial activities with its preservative effect against Listeria monocytogenes inoculated in minced beef meat. Lipids Health Dis. 2017; 16(1):1-11. https://doi.org/10.1186/ s12944-017-0487-5 PMid:28774297 PMCid:PMC5543433
- Steventhie L, Parmadianti AE, Ayuningtyas NF. Efektivitas minyak esensial kulit buah jeruk lemon (Citrus limon) Terhadap pertumbuhan jamur candida tropicalis secara invitro (in vitro, effectivity of lemon peel essential oil (Citrus limon) against candida tropicalis). Oral Med Dent J. 2016; 8(2):25- 30.
- Syarifah IM, Ernawati DS, Hernawan I. Efektivitas pemberian minyak esensial kulit buah Citrus limon l. 0,78% topikal terhadap peningkatan jumlah makrofag pada penyembuhan ulkus traumatikus tikus wistar diabetik. Skripsi Fak Kedokt Gigi Univ Airlangga. 2017.
- Coico R, Sunshine G. Immunology: A short course. 7th ed. Chichester: John Wiley & Sons, Ltd.; 2015. p. 3-4, 97-105.
- Actor JK, Ampel NM. Hypersensitivity: T Lymphocytemediated (Type IV). Encyclopedia of Life Sciences; 2009. p. 3-4, 97-105.
- Bakula A, Lugović-Mihić L, Situm M, Turcin J, Sinković A, Lugovic Mihic L, Sinkovic A. Contact allergy in the mouth: Diversity of clinical presentations and diagnosis of common allergens relevant to dental practice. Acta Clinica Croatica. 2011; 50(4): 553-61.
- Buttaravoli P, Hudson P. Part 11 dermatologic emergencies in minor emergencies. 2012. p. 639 https://doi.org/10.1016/ B978-0-323-07909-9.00160-4
- Popovic J, Cvetkovic T, Dzopalic T, et al. The concentration of tumor necrosis factor alpha in periapical lesions. Stomatol Glas Srb. 2014; 61(1):7-13. https://doi. org/10.2298/SGS1401007P
- Mahdani FY, Parmadiati AE, Ernawati DS, Husain H, Ekaperdana SAP, Rachmaningayu U, Hadi P, Hendarti HT, Surboyo MDC. Citrus limon peel essential oil-induced Type IV hypersensitivity reaction. J Exp Pharmacol. 2020; 12:213- 20. https://doi.org/10.2147/JEP.S256139 PMid:32801948 PMCid:PMC7394596
- Wong Y, Ahmad-Mudzaqqir M, Wan Nurdiyana W. Extraction of essential oil from cinnamon (Cinnamomum zeylanicum). Orient J Chem. 2014; 30(1):37-47. https://doi.org/10.13005/ojc/300105 18. Sharma B, Singh LR, Pradesh U, Pradesh U. Pharmaceutical gels for topical drug delivery: An overview. Int J Res Pharm Pharm Sci. 2018; 3(2):19-24.
- Tjahyono, N, Parmadiati, AE, Mahdani, FY. Uji sitotoksisitas minyak esensial kulit jeruk lemon (Citrus Limon) terhadap sel human gingival fibroblas. Oral Medicine Dental Journal. 2017; 9(1):53-60.
- Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils chemical characterization and investigation of some biological activities: A critical review. Medicines. 2016; 3(4):25. https://doi.org/10.3390/medicines3040025 PMid:28930135 PMCid:PMC5456241
- Jongedijk E, Cankar K, Buchhaupt M, Schrader J, Bouwmeester H, Beekwilder J. Biotechnological production of limonene in microorganisms. Applied Microbiology and Biotechnology. 2016; 100(7):2927- 38. https://doi.org/10.1007/s00253-016-7337-7 PMid:26915992 PMCid:PMC4786606
- Siddique AB, Rahman SMM, Hossain MA. Chemical composition of essential oil by different extraction methods and fatty acid analysis of the leaves of Stevia Rebaudiana Bertoni. Arabian Journal of Chemistry. King Saud University. 2016; 9:S1185-9. https://doi.org/10.1016/j.arabjc.2012.01.004
- Liu H. Comprehensive natural products II: Chemistry and Biology. 1st ed. United Kingdom: Elsevier Ltd; 2010.
- Silveira E Sá RDCD, Andrade LN, De Sousa DP. A review on anti inflammatory activity of monoterpenes. Molecules. 2013; 18(1):1227-54. https://doi.org/10.3390/molecules18011227 PMid:23334570 PMCid:PMC6269770 25. Shahbazi S, Bolhassani A. Immunostimulants : Types and functions. J Med Microbiol Infec Dis. 2017; 4(69):45-51.
- Audrain H, Kenward C, Lovell CR, Green C, Ormerod AD, Sansom J, Chowdhury MMU, Cooper SM, Johnston GA, Wilkinson M, King C, Stone N, Horne HL, Holden CR, Wakelin S, Buckley DA. Allergy to oxidized limonene and linalool is frequent in the UK. The British Journal of Dermatology. 2014; 171(2):292-7. https://doi.org/10.1111/ bjd.13037 PMid:24702129
- Oliver B, Krishnan S, Rengifo Pardo M, Ehrlich A. Cosmeceutical contact dermatitis-cautions to herbals. Current Treatment Options in Allergy. 2015; 2(4):307-21. https://doi.org/10.1007/s40521-015-0066-9
- Christensson JB, Karlberg AT, Andersen KE, Bruze M, Johansen JD, Garcia-Bravo B, White IR. Oxidized limonene and oxidized linalool- Concomitant contact allergy to common fragrance terpenes. Contact Dermatitis. 2016; 74(5):273-80. https://doi.org/10.1111/cod.12545 PMid:26918793
- Raffalli C, Clouet E, Kuresepi S, Damiens MH, Lepoittevin JP, Pallardy M, Kerdine- Römer S. Fragrance allergens linalool and limonene allylic hydroperoxides in skin allergy: Mechanisms of action focusing on transcription factor Nrf2. Toxicological Sciences. 2017; 1-10. https://doi. org/10.1093/toxsci/kfx207 PMid:29029310 30. Sudiono J. Sistem Kekebalan Tubuh. Jakarta: EGC; 2014.
- Abbas AK, Lichtman AH, Pillai S. Basic immunology: Functions and disorders. 5th ed. Missouri: Elsevier; 2016. p. 232-3, 243-4.
- Giamarellos-Bourboulis EJ, Raftogiannis M. The immune response to severe bacterial infections: Consequences for therapy. Expert Rev Anti Infect Ther. 2012; 10(3):369-80. https://doi.org/10.1586/eri.12.2 PMid:22397569
- Murphy K. Janeway’s Immunobiology. 8th ed. New York: Garland Science, Taylor & Francis Group; 2012. p. 571, 588- 9, 600-3.
- Subowo. Imunologi Klinik. Edisi Ke-2. Jakarta: Sagung Seto; 2013.
- Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology. 8th ed. Philadelphia: Elsevier Saunders; 2015. p. 216-21, 400, 405-9.
- Astuti DP. Formulasi dan Uji Stabilitas Fisik Sediaan Gel Antiseptik Tangan Minyak Atsiri Bunga Lavender (Lavandula angustifolia Miller). Jurnal Farmaka. 2017; 15(1):176- 84.
- Marques-Marinho FD, Vianna Soares CD. Establishment of Artificial Human Sera (ARTHUS) based on chimeras of Fcgamma RI and human immunoglobulin domains. Allergy: European Journal of Allergy and Clinical Immunology. 2013; 141-62.
- Maulina L, Sugihartini N. Formulasi gel ekstrak etanol kulit buah manggis (Garcinia mangostana L.) dengan variasi gelling agent sebagai sediaan luka bakar. Pharmaciana. 2015; 5:43-52. https://doi.org/10.12928/pharmaciana. v5i1.2285
- Johansen JD. Fragrance contact allergy: A clinical review. American Journal of Clinical Dermatology. 2003; 4(11):789- 98. https://doi.org/10.2165/00128071-200304110-00006 PMid:14572300
- Nath NS, Liu B, Green C, Atwater AR. Contact allergy to hydroperoxides of linalool and D-Limonene in a US population. Am Contact Dermat Soc. 2017; 28(5):313-6. https:// doi.org/10.1097/DER.0000000000000318 PMid:28885310