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Ramani, Pratibha
- Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain
Abstract Views :237 |
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Authors
Aiman Zafar
1,
Pratibha Ramani
1,
Herald J. Sherlin
1,
Gheena
1,
R. Abhilasha
1,
Giffrina Jayaraj
1,
K. R. Don
1,
S. Archana
1
Affiliations
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, IN
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 79-82Abstract
Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is requiredKeywords
Stains , Modified Stains.References
- Anderson J. (2011). An introduction to Routine and special staining. Retrieved on August 18, 2014 from. Godwin, A. (2011).
- Histochemical uses of haematoxylin-a review. Retrieved August 18, 2014 From www.arpapress.comHarris, T. J., & McCormick, F. (2010).
- The molecular pathology of cancer. Nat Rev Clin Oncol, 7(5), 251-256.
- Liquid Paraffin as a Rehydrant for Air Dried Buccal Smear
Abstract Views :243 |
PDF Views:0
Authors
R. Hannah
1,
Pratibha Ramani
1,
M. P. Brundha
2,
Herald J. Sherlin
1,
Gheena Ranjith
1,
Abilasha Ramasubramanian
1,
Gifrina Jayaraj
1,
K. R. Don
1,
S. Archana
1
Affiliations
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1197-1200Abstract
Aim: To assess the efficacy of liquid paraffin as a rehydrant for air dried buccal smear based on the nuclear and cytoplasmic details. Background: Buccal smear is useful for diagnosing Malignancy, Fungal infection, Viral infection and Vesicullobullous dermatoses. The routine practice is to wetfixthe smear and send them to the laboratory for staining and evaluation by a cytopathologist. But drying of smears is inevitable, especially when the aspirate is less and when there is improper fixation. This can cause drying artifacts. An alternative method for overcoming this problem is intentional air drying followed by rehydration. Many rehydrants have been experimented upon. The most common rehydrant being saline. The present study is done to check the efficacy of liquid paraffin as a rehydrant. Material and methods: 2 sets of 20 buccal smears were collected from the patients. One set air dried for 24 hours and the other wet fixed. Conventional pap staining was then carried out. The slides were examined by two observers for preservation of Nuclear and cytoplasmic details based on the semi quantitative scoring system. Results: Excellent nuclear details were seen in 45% of air dried smear compared to 25%in routine wet fixation. The cytoplasmic details of air dried smear and routine wet fixed smear was the same. Conclusion: Liquid paraffin as a rehydrant has shown promising results and the nuclear details were found to marginally better than in routine wet fixed smear. Air dried buccal smears can be used in routine practice especially in rural areas where there is limited access to laboratories and for mass screening.Keywords
Buccal smear, Rehydration, Liquid Paraffin.References
- Hoda R.S., Hoda S.A. Artifacts, contaminants and incidental findings. In: Fundamentals of Pap Test Cytology. Humana Press 2007; 179-186.
- Lencioni LJ, Staffieri JJ, Cardinnet LJ. Vaginal and urinary sediment smear staining technique without previous fixation; adapted to Papanicolaou’s and Shorr’s staining methods. J Lab Clin Med.1954; 44(4): 595-9.
- Chan JKC, Kung ITM. Rehydration of airdried smears with normal saline: application in fine-needle aspiration cytologic examination. Am J Clin Pathol 1988; 89(1):30-4.
- M. S. Israel, L.F. Young. Use of liquid paraffin in the preservation of pathological specimens. J Clin Path.1978; 31(5):499-500.
- Crothers, Barbara and Henry, Michael and Firat, Pinar and Hamper, Ulrike. Nondiagnostic/Unsatisfactory: The Bethesda System for Reporting Thyroid Cytopathology. 2010.
- Ahmed HG, Tom MA. The consequence of delayed fixation on subsequent preservation of urine cells. Oman Medical Journal. 2011; 26(1):14-8.
- Zare –Mirzaie, K. Kalili-Alam and M. Abolhasani. Rehydration of air-dried cervical smears: An alternative to routine wet fixation. Acta Medica Iranica 2007; 45(5):365-68.
- Agar-Paraffin Double Embedding Over Conventional Embedding for Minute Oral Biopsies-Cohort Study
Abstract Views :527 |
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Authors
Affiliations
1 Postgraduate, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
2 Professor and Head of the Department, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
3 Professor, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
4 Reader, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
5 Senior Lecturer, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
1 Postgraduate, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
2 Professor and Head of the Department, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
3 Professor, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
4 Reader, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
5 Senior Lecturer, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 14, No 1 (2020), Pagination: 84-88Abstract
Agar-Paraffin double embedding technique is a simple technique that combines the advantages of both the embedding material. It preserves the minute tissue biopsies in orientation and holds them together from getting lost. Oral biopsies, being most commonly small incisional tissues, have to be preserved all through the processing and embedding to ensure optimal visualization of all the mucosal layers without compromise. In the present study, samples were divided into two groups: Group A: Agar-paraffin double embedding (APE) and Group B: conventional paraffin embedding (PE). Tissue samples collected were sectioned into two approximately equal sized bits. Both the bits were simultaneously processed, embedded in two different techniques (APE and PE method). Sections obtained were scored by an observer and analyzed using independent sample t-test (SPSS software version 21) to evaluate the efficacy of agar-paraffin double embedding technique in comparison to the conventional paraffin embedding technique. Agar paraffin embedded tissue was found to be well processed, firm and well preserved. Orientation was comparatively easier and the blocks yielded sections of good quality. They showed no interference with staining and cell morphology was of good clarity. Thus Agar-paraffin embedding technique represents a simple, reliable method that can greatly improve the quality of diagnostic information.Keywords
Agar-Paraffin, Double Embedding, Oral Biopsies.- Knowledge about Legal Aspects of Medical Negligence in India among Dentists–A Questionnaire Survey
Abstract Views :517 |
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Authors
Affiliations
1 III Year MDS, Post Graduate Student, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
2 Professor and Head, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
3 Professor, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
4 Reader, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
5 Senior Lecturer, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
1 III Year MDS, Post Graduate Student, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
2 Professor and Head, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
3 Professor, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
4 Reader, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
5 Senior Lecturer, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN