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- G. Senthilkumar
- A. Josphine
- A. Panneerselvam
- A. Vinoth
- V. Balakrishnan
- R. Kalaivani
- N. Nadimuthu
- S. Suganya
- R. Bharathidasan
- S. Manorajan
- K. Kaviyarasi
- A. Cholarajan
- A. Prathiba
- P. Sumithra
- T. Viji
- S. Gnanavel
- R. Mahalingam
- K. Kanimozhi
- V. Ambikapathy
- A. Gayathri
- K. Ramanathan
- N. Arun
- M. Mariyappan
- V. Saranya
Journals
- Research Journal of Pharmacology and Pharmacodynamics
- Research Journal of Science and Technology
- Asian Journal of Pharmaceutical Research
- Asian Journal of Pharmaceutical Analysis
- Asian Journal of Pharmacy and Technology
- Research Journal of Pharmaceutical Dosage Form and Technology
- Asian Journal of Research in Chemistry
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Madhanraj, P.
- Antibacterial Activity of Aspergillus oryzae against some Human Pathogens
Abstract Views :241 |
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Authors
Affiliations
1 Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt.) - 613 503, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Elambalur, Perambalur - 621 212, Tamil Nadu, IN
1 Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt.) - 613 503, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Elambalur, Perambalur - 621 212, Tamil Nadu, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 3, No 5 (2011), Pagination: 250-252Abstract
The marine fungi occurring on the wood samples were studied. Diversity of marine fungi colonizing the lignicolous wood samples was assessed and their distribution pattern was determined. The antibacterial activity of Aspergillus oryzae was tested against five human pathogens by agar well diffusion method. Based on the results, the compounds were separated by Thin Layer Chromatography (TLC) and these compounds were tested against three human bacterial pathogens. The separated compounds were further analyzed by Fourier Transform Infrared Spectroscopy (FTIR) to identify the functional groups, for the antibacterial activity.Keywords
Aspergillus oryzae, FTIR, TLC, Sterol.
References
- Annette, N. J., 2009. A novel procedure for strain classification of fungal mycelium By cluster and artificial neural network analysis of Fourier Transform infrared(FTIR) spectra. Analyst.134: 1215-1223.
- Bugni. T.S., Ireland, C.M., 2004. Marine derived fungi a chemically and biologically diverse group of microorganisms. Nat. Prod. Rep. 21: 143-163.
- Korzybski,T., Kowszyk - Gindifer, and Kurylowicz., 1967. Antibiotics, Vol.2. Pergamon press, New York.
- Perez, C., Paul, M., Bazerque, P., 1990. Antibiotic assay by agar well diffusion method. Acta. Biol. Med. Exp., 15: 113-115.
- Singh, R., Sharma, M., Joshi, P., Rawat, D.S., 2008. Clinical status of anti-cancer agents derived from marine sources. Anticancer agents Med Chem. 8: 603-617.
- Wagner, R. and Bladt, S.D.,1996. Plant Drug Diagnosis, A thin layer chromatography, pp:384.
- Yanagihara, N., Sasaki-Takahashi, M., Sugahara, T., Yamamoto, S., Shinomi, M., Yamashita, E., Hayashida, M., Andoh, T., 2005. Leptosins isolated from marine fungus Leptosphaeraia species inhibit DNA topoisomerases I and/or II and induce apoptosis by inactivation of AKT/Protein Kinase B. Cancer. Sci. 96: 816-824.
- Young, X., Jun, L., An-guol., and Jing-Xia, C., 2003.Structure identification for the compound I separated and purified from taxoids produced endophytic fungi (Alternaria alternate var. taxi 1011) J.For.Res.14(3) : 217-233.
- FTIR Spectrum Characteristic of Treated Spent Oil with Fungi
Abstract Views :197 |
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Authors
Affiliations
1 Department of Biotechnology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN
1 Department of Biotechnology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN
Source
Research Journal of Science and Technology, Vol 6, No 4 (2014), Pagination: 185-193Abstract
Oil pollution is worldwide major problem in every environment and hence the world is need of a perfect solution for prevention or recovery so, the present investigation was carried out to detect the spent lubricant oil degradation potentional of indigenous fungi in lab level by measuring the growth and analysis the treated sample using FTIR. Soil sample was randomly collected in the different location at railway tracks in Thanjavur junction soil sample are serially and 10-2 is used for plating technique in PDA medium. After incubation the isolates were obtained and they are Aspergillus terreus, Aspergillus flavus and Aspergillus niger. Oil degrading ability was detected supplying raw spent oil in PDA medium and incoprporated in PD broth at the concentration of (5%.10%, 15% and 20%), detection shows that no fungal cored use raw oil as source of nutrient. In PDA plate's visual detection of zone due to the degradation was noticed. It was maximum by Aspergillus flavus follwed by Aspergillus terreus and Aspergillus niger. In PD broth supplemented with different spent oil concentration (5%,10%,15% and 20%), after 14 days of incubation (30°C), as a visual the biomass of each fungal culture was determined the biomass of different species of fungi in different concentration oil showed growth but with variation the higher mycelial biomass was recorded by Aspergillus flavus. The FT-IR analysis results shows that is major difference in the peak formation between the tested samples which shows that fungal species has utilized or degraded the oil hydrocarbon. Differently as per their metabolize activity.- Antibacterial Activity of Essential Oil Extracted from Coriandrum sativam (L.) and GC-MS Analysis
Abstract Views :152 |
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Authors
Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Research Journal of Science and Technology, Vol 4, No 5 (2012), Pagination: 203-207Abstract
Extraction of essential oils from locally available plant Coriandrum sativam (L.) was carried out using steam distillation followed by ether extraction. Dried and purified extracted oils were screened for their antibacterial activity of both gram positive (Staphylococcus aureus) and gram negative (Enterobacter aerogenes, Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) bacterial strains by Agar well diffusion method. Coriandrum sativam shows antibacterial activity against the above given five bacterial strains. GC-MS was done on Coriandrum sativam oil. These results support that this plant oil can be used to cure bacterial infections and may also have role as pharmaceuticals and preservatives.Keywords
Coriandrum sativam, GC-MS, Essential Oil, Antibacterial Activity and Extraction.- Studies on the Fungi in Seasonally Inundated Sediment Habitat along South East Coast of India
Abstract Views :183 |
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Authors
Affiliations
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN
Source
Research Journal of Science and Technology, Vol 3, No 4 (2011), Pagination: 175-179Abstract
In seasonally inundated sediments of Manianthivu of Nagapattinam district, southeast coast of India, a total number of 24 species belonged to 5 genera were recorded. They were assignable to Phycomycete (1 species) and Deuteromycetes (23 species). They exhibited spatio-temporal changes. The genus Aspergillus was constituted by the maximum of 19 species followed by Trichoderma (2 species). All other genera (Absidia, Botrytis and Fusarium) were represented by one species each. The soil characteristics such as pH (8.1 to 8.9), electrical conductivity (0.11 to 1.28 dSm-1), cation exchange capacity (8.01 to 9.5 c.mol proton+/kg), organic carbon (0.05 to 0.18%), available nitrogen (0.012 to 0.018%), available phosphorus (0.002 to 0.005%), available potassium (0.013 to 0.047 ppm), available zinc (0.39 to 0.57 ppm), available iron (2.12 to 2.83 ppm), available copper (0.19 to 0.31 ppm), available manganese (1.41 to 1.52 ppm), calcium (3.2 to 5.2 mg/kg), magnesium(3.2 to 3.9 mg/kg), sodium (0.52 to 1.22 mg/kg) and potassium (0.02 to 0.07 mg/kg) were also showed variation during different seasons. The investigation was carried out by collections and examination of sediment samples during January 2007 - December 2008, at seasonal intervals.Keywords
Fungi, Soil Characteristics, Inundated Sediment.- Effect of PGPR Studies on Some Angiospermic Plants
Abstract Views :171 |
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Authors
Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
Source
Research Journal of Science and Technology, Vol 3, No 2 (2011), Pagination: 76-79Abstract
PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.Keywords
PGPR, Pseudomonas fluorescence.- Cultivation, Composting, Biochemical and Molecular Characterization of Pleurotus platypus (Cooke and Massee) Sacc
Abstract Views :166 |
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Authors
Affiliations
1 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu, IN
2 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu., IN
1 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu, IN
2 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu., IN
Source
Research Journal of Science and Technology, Vol 3, No 5 (2011), Pagination: 284-287Abstract
In the present investigation, the mushroom yield, chemical composition, nutritional value of Pleurotus platypus cultivated in paddy straw, black gram stem and black gram pods were determined. Fresh mushroom yield amounts obtained from paddy straw, black gram stem and black gram pods substrate were 940, 560 and 595 g / kg respectively. Mushroom cultivated on different substrates were analyzed for protein, carbohydrate, amino acids and fatty acid content. The cellulolytic activity and coir pith compost was also investigated in Pleurotus platypus. Hence P. platypus was also selected for molecular studies. Therefore, the present results suggest it could be used to recycle the agro – waste materials.Keywords
Pleurotus platypus, Chemical Composition, Nutritive Value, Cellulolytic Activity, Coir Pith Compost.- Ecology and Biocontrol Potential of Soil Fungi of a Backwater Environment along the East Coast of India
Abstract Views :203 |
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Authors
Affiliations
1 Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirappalli - 620 005, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN
1 Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirappalli - 620 005, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN
Source
Asian Journal of Pharmaceutical Research, Vol 5, No 1 (2015), Pagination: 24-30Abstract
The soils collected from a backwater environment at Vellapallam in Nagapattinam district of Tamilnadu state along the east coast of India during May 2013 were subjected to the examination of fungi on PDA medium and physicochemical characteristics. The culturable propagules of the fungi were in the range from 6.98 to 106 x 102 CFU/g and the species diversity (number of species) was from 5 to 9.
The pH of the soil was in the range from 7.9 to 8.9; electrical conductivity from 0.04 to 1.31 dSm-1; cation exchange capacity from 8.01 to 10.11 c.mol proton+/kg; organic carbon from 0.04 to 0.21%; available nitrogen from 0.011 to 0.22%; available phosphorus from 0.001 to 0.006%; available potassium from 0.011 to 0.057 ppm; available zinc from 0.36 to 0.57 ppm; available iron from 2.01 to 2.91 ppm; available copper from 0.14 to 0.34 ppm; available manganese from 1.22 to 1.53 ppm; calcium from 3.1 to 5.7 mg/kg; magnesium from 3.1 to 4.2 mg/kg; sodium from 0.29 to 1.73 mg/kg; and potassium 0.01 to 0.08 mg/kg. Among them electrical conductivity and the Organic Matter showed significant positive correlation with the total fungal population.
All the fungal species that were isolated from soils were tested against F. semitectum, a known soil borne fungal pathogen, by dual culture and food poisoning methods. Among them Trichoderma koeningii showed promising activity against the pathogenic fungus for the maximum both in dual culture and in food poisoning techniques. The inhibition was 61.3% at 20% concentration. As the antagonistic fungus was isolated from the environment that showed typical marine conditions, the isolate could better serve as biocontrol agent to control the soil born pathogen, F. semitectum in saline soil crop fields.
- Diversity of Fungi from Drift Wood of Muthupet Mangroves
Abstract Views :215 |
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Authors
Affiliations
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 1, No 3 (2011), Pagination: 53-55Abstract
The variety and galaxy of fungi and their natural beauty occupy prime place in the biological world and India has been the cradle for such fungi. Only a fraction of total fungal wealth has been subjected to scientific scrutiny and mycologist have to unravel the unexplored and hidden wealth. The marine fungi occurring on the wood samples collected from the inter tidal region of the Muthupet mangrove environment were studied. Diversity and distribution of different organisms in the marine environment are influenced by the physico-chemical properties of the soil. Totally 23 fungal species were recorded.Keywords
Fungi, Diversity, Wood Samples, Mangroves.- Antimicrobial Activity of Strychnos nux-vomica Linn and Cassia angustifolia Linn
Abstract Views :238 |
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Authors
Affiliations
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 2, No 1 (2012), Pagination: 8-11Abstract
The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.- Isolation, Identification and Molecular Characterization of Phosphate Solubilizing Actinomycetes Isolated From The Coastal Region of Manora, Thanjavur (Dt.)
Abstract Views :173 |
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Authors
Affiliations
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 4 (2011), Pagination: 119-122Abstract
The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.Keywords
Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.- Diversity, Antibacterial Activity And Molecular Characterization of Actinomycetes Isolated From Salt Pan Region of Kodiakarai, Nagapattinam DT.
Abstract Views :198 |
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Authors
Affiliations
1 Department of Botany and Microbiology, A. V. V. M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
1 Department of Botany and Microbiology, A. V. V. M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 3 (2011), Pagination: 79-81Abstract
The present study was to isolate and identify antimicrobial activity producing actinomycetes strains from marine habitat, to find out antibacterial efficiency against the common human pathogens. Marine actinomycetes were isolated from salt pan region of Kodiakarai, Nagapattinam [District].Out of 20 isolated actinomycetes 10 were identified and selected for antibacterial activity. Out of 10 dominant actinomycete species Streptoverticillium album was highly dominant and showed the best level of antibacterial activity against three human pathogens, Staphylococcus aureus, Klebsiella pheumoniae and Escherichia coli. The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics and also confirmed by molecular characterization.Keywords
Antibacterial Activity, Sterptoverticillium album, Marine Actinomycetes.- Studies on the Compound Separation and Its Antifungal Potentiality of Fungi Isolated From Nagapattinam District, East Coast of India
Abstract Views :183 |
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Authors
Affiliations
1 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi- 613 503, Thanjavur Dt., Tamil Nadu, IN
2 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) – 613 503, Tamil Nadu, IN
1 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi- 613 503, Thanjavur Dt., Tamil Nadu, IN
2 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) – 613 503, Tamil Nadu, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 3, No 3 (2011), Pagination: 100-104Abstract
Altogether 37 species belonged to 10 genera were recorded. A preliminary screening of all the species isolated from soils were used for antifungal (antagonistic) activity against Fusarium semitectum, a known soil borne fungal pathogen. Among the species tested, Trichoderma koeningii showed promising activity, inhibited the pathogenic fungus for the maximum both in dual culture and in food poisoning technique. When HPLC fractionation of the mycelial extract of T. koeningii grown in PDA broth yielded three fractions. They were named as peak 1, peak 2 and peak 3.Among the three fractions, peak 2 alone showed the antifungal activity with the inhibition zone of 13 mm diameter against Fusarium semitectum. The functional groups present in peak 1 and 3 are almost same, but in the peak 2 some extra functional groups are present. These groups may be responsible for the antifungal activity of this fraction.
Keywords
Antifungal Activity, Dual Culture and Food Poisoning Technique, HPLC, Compounds, Biocontrol.- Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum
Abstract Views :182 |
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Authors
Affiliations
1 Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
1 Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 8 (2011), Pagination: 1225-1230Abstract
The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.Keywords
Trichoderma harzianum, DNA Isolation, Evolution.- Atomic Study on Fusarium incarnatum using Bioinformatics Tools
Abstract Views :167 |
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Authors
Affiliations
1 Department of Microbiology, Thanthai Hans Roever College, Perambalur-621212, IN
2 Department of Bioinformatics, Thanthai Hans Roever College, Perambalur-621212, IN
3 Department of Biochemistry, Thanthai Hans Roever College, Perambalur-621212, IN
4 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
5 Department of Botany and Microbiology, A.V.V.M Sri Puspam College, Poondi, Thanjavur–613503, Tamil Nadu, IN
1 Department of Microbiology, Thanthai Hans Roever College, Perambalur-621212, IN
2 Department of Bioinformatics, Thanthai Hans Roever College, Perambalur-621212, IN
3 Department of Biochemistry, Thanthai Hans Roever College, Perambalur-621212, IN
4 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
5 Department of Botany and Microbiology, A.V.V.M Sri Puspam College, Poondi, Thanjavur–613503, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 2, No 2 (2012), Pagination: 78-82Abstract
We have been investigating that the atomic study of Fusarium incarnatum with the utilization of Bioinformatics tools. We have been involved in the insilico study on the plant pathogen the Fusarium incarnatum. The involvement of Nucleotides and atoms in this species can be identified with the help of Bioinformatics tools. We can also involved in the analysis of amino acids which are present in this species and calculated the start and stop codons in different frames.Keywords
Fusarium incarnatum, Bioinformatics Tools, Nucleotides, Atomic Study, Amino Acids, Frame Analysis.- Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla
Abstract Views :165 |
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Authors
M. Mariyappan
1,
R. Bharathidasan
1,
R. Mahalingam
1,
P. Madhanraj
2,
A. Panneerselvam
1,
V. Ambikapathy
1
Affiliations
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 4 (2011), Pagination: 111-113Abstract
The bacterial organisms were isolated from drinking water (Bacillus, Borchothrix, Clavibacter sp, Ancylobacter sp, and Brevi bacterium). Selected Indian medicinal plants Cardiospermum halicacabum and Melothria heterophylla were selected for antibacterial studies. The solvents used for the extraction of plant ischolar_mains were n- butanol, ethyl acetate and distilled water. The invitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Postive bacteria was Bacillus sp, Brevibacterium sp, and the most susceptible Gram-negative bacteria was Borchothrix sp, Clavibacter sp, and Ancylobacter sp. The extracts of plant Cardiospermum halicacabum and Melothria heterophylla inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that Cardiospermum halicacabum and Melothria heterophylla could be used in treating diseases caused by the test organisms. The results are discussed in detail.Keywords
Medicinal Plants, Antibacterial Activity, Aqueous Extract, N-Butanol Excretory Ethyalcetate Extract.- Cultivation, Composting, Biochemical and Molecular Characterization of Calocybe indica (C and A)
Abstract Views :184 |
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Authors
Affiliations
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi, Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi, Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 3 (2011), Pagination: 55-57Abstract
Investigation on the cultivation of milky mushroom, Calocybe indica was conducted to find out the growth and yield performance on different substrates. The yield of fresh mushroom obtained from paddy straw, teak leaves and sugarcane trash substrates were 1140, 745 and 570 g/kg respectively. The nutritional significance of Calocybe indica cultivated on different substrates was assessed in terms of protein, carbohydrate, amino acids and lipids. The cellulolytic activity and coir pith compost was also tested using Calocybe indica. The present result suggest, the lignocellulosic substrate like paddy straw, teak leaves and sugarcane trash shown great potential for use as a raw material since this substrate provides an economically acceptable production for Calocybe indica.Keywords
Calocybe indica, Sugarcane Trash, Teak Leaves, Composting of Coir Pith.- Mycoecology in the Soil of a Casuarina Bioshield Plantation along Southeast Coast of India
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Authors
Affiliations
1 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur, Tamil Nadu, IN
2 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
3 P.G. and Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt.), Tamilnadu, IN
1 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur, Tamil Nadu, IN
2 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
3 P.G. and Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt.), Tamilnadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 2 (2011), Pagination: 37-41Abstract
A total number of 325 colonies were isolated by plating method from the soils of Casurina bioshield plantation at Naluvedhapathy (Nagapattinam Dt.), along south east coast of India. There were 19 species belonged to 4 genera with Aspergillus as the dominant genus, represented by more number of species (14 species), followed by Fusarium and Trichoderma (2 species each). Population density varied from 9.7 to 17.7 CFU/g and the species diversity from 6 to 10 species during different stations. Physico-chemical analyses of the soils revealed the following features: pH 7.9 to 8.8, electrical conductivity 1.06 to 1.31 dSm-1, cation exchange capacity 8.67 to 10.11 c.mol proton+/kg, organic carbon 0.04 to 0.21%, available nitrogen 0.011 to 0.022%, available phosphorus 0.002 to 0.005%, available potassium 0.013 to 0.051 ppm, available zinc 0.47 to 0.57 ppm, available iron 2.13 to 2.90 ppm, available copper 0.22 to 0.31 ppm, available manganese 1.38 to 1.53 ppm, calcium 3.1 to 5.1 mg/kg, magnesium 3.2 to 4.1 mg/kg, sodium 0.23 to 0.79 mg/kg and potassium 0.02 to 0.08 mg/kg, during different seasons.Keywords
Soil Fungi, Bioshield Plantation, Soil Characteristics, Mycoecology.- Studies on the Compounds and its Antifungal Potentiality of Fungi Isolated from Paddy Field Soils of Jenbagapuram Village, Thanjavur District, and South India
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Authors
Affiliations
1 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt), Tamil Nadu, IN
1 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt), Tamil Nadu, IN