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S. Selvi , P. Umadevi , D. Devipriya , P. Chinnaswamy

Abstract

The ethanolic extract of Calocybe indica was studied for its free radical scavenging property on different in vitro models like 1, 1 - diphenyl-picryl hydrazyl (DPPH) Assay, Ferric Reducing Antioxidant Power (FRAP) assay and in vitro antilipidperoxidative assay using goat liver homogenate and RBC Ghost model. The in vitro Lipid peroxidation (LPO) was inhibited to a good extent by the Calocybe indica ethanolic extract and the extent of inhibition being higher in the RBC membrane model than the liver homogenate model. The mushroom extract showed good dosedependent free radical scavenging property in both the models.

Keywords

Free Radical Scavenging, Calocybe indica, Antioxidant

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K. Jeyaprakash , P. Chinnaswamy

Abstract

Objective: The present study was carried out to evaluate the antioxidant property of spirulina and Liv-52 against lead induced toxicity in albino rats. Materials and methods: The antioxidant property of spirulina and Liv-52 was investigated by using lead acetate to induce toxicity in albino rats. The extent of lipid peroxidaiton in terms of thiobarbituric acid reactive susbstances (TBARS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathine (GSH) were assayed in the liver and kidney homogenate. Results: Oral administration of lead (10mg/kg body weight/day) as lead acetate for 30 days resulted in a significant increase (P<0.01) in the level thiobarbituric acid reactive substances (TBARS) and a decrease in the level of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) when compared to normal control. Administration of both spirulina and Liv-52 produced a pronounced protective effect (P< 0.01) in respect to these parameters when compared to their individual administration in lead intoxicated rats. Conclusion: The results of the present study suggested the antioxidant and protective efficacy of spirulina and Liv-52 against lead induced toxicity in albino rats.

Keywords

Thiobarbituric Acid Reactive Substances (TBARS), Lead Intoxicated Rats, Protective Efficacy

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S. Seethalakshmi ¹, P. Chinnaswamy ²

Affiliations
1 Department of Biochemistry, CMS College of Science and Commerce, Coimbatore - 641 006, Tamil Nadu, IN
2 Dr N.G.P. Arts and Science College, Coimbatore - 641 035, Tamil Nadu, IN

Abstract

Alcoholism is a primary chronic disease with genetic psychosocial and environmental factors influencing its development and manifestation. The disease is often progressive and fatal. The World Health Organisation no longer recommends the term alcoholism but prefers the term alcohol dependence syndrome.

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K. Selvam ¹, K. Swaminathan ², M. Manikandan ¹, P. Chinnaswamy ³

Affiliations
1 Department of Biotechnology, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N., IN
2 Department of Biotechnology, Bharathiar University, Coimbatore-641 046, T.N., IN
3 Department of Biochemistry, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N, IN

Abstract

White rot fungus, Thelephora, isolated by its high production levels of ligninolytic enzymes from stumps of a burnt tree, was examined for its optimum growth conditions such as pH and temperature as well as carbon sources (glucose, cellulose and lignin) and nitrogen sources (diammonium tartrate, yeast extract and urea). For the maximum growth of Thelephora the 6 day incubation period was required at pH 5.0 and 35°C with 3.0 g/L lignin as a carbon source and 0.88 g/L yeast extract as a nitrogen source. The lignin peroxidase (LiP) production was maximal on the 7th day at pH 4.5 and 40°C with 3.0 g/L lignin and 0.44 g/L diammonium tartrate. The manganese-dependent peroxidase (MnP) production was achieved on 7th day incubation at pH 4.5 and 40°C, with 2.5 g/L glucose and 0.22 g/L diammonium tartrate as carbon and nitrogen sources respectively. The highest amount of laccase was obtained on the fourth day of incubation at pH 7.0 and 35°C using 3.0 g/L cellulose and 0.44 g/L diammonium tartrate as carbon and nitrogen sources respectively.

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P. Uma Devi ¹, S. Murugan ¹, S. Akilapriyadharasini ², S. Suja ², P. Chinnaswamy ³

Affiliations
1 Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore-641 014, T. N, IN
2 Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore-641 014, T. N, IN
3 Institute of Laboratory Medicines, Kovai Medical Center and Hospitals, Coimbatore-641 014, IN

Abstract

The lethal toxicity tests for mercury and effluents were performed in Vigna unguiculata (cow pea). There was a significant alteration in the growth of plants. Effects of mercury and effluents reduce the germination of seeds. An abnormality in ischolar_main-shoot length as well as variation in amylase activity was observed. The amount of phenolic compounds increase with increase in mercury content. Since Vigna unguiculata is an annual plant, results can be seen quickly.

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