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D. K. Pal , S. Kumar , P. Chakrabarty , M. Kumar

Abstract

Objective: To study the antioxidant activity of different extracts of nuts of Semecarpus anacardium in vitro. Methods: The in vitro antioxdant activity was investigated by two methods: by estimating degree of non-enzymatic haemoglobin glycosylation measured colorimetrically at 520 nm and by assaying DPPH free radical scavenging activity. Results: It was found that petroleum ether and ethanol extracts of nuts of S. anacardium showed higher antioxidant activity than other extracts of it. The antioxidant activities of the extracts are close and identical in magnitude and comparable to that of standard antioxidant compounds used. The antioxidant activities of the extracts were concentration dependant. Conclusion: The results of the present study justify the use of the nuts as folk remedies.

Keywords

Antioxidant Activity, Semecarpus anacardium Nuts, Non-enzymatic Haemoglobin Glycosylation, DPPH Free Radical Scavenging Activity

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M. Duttachoudhury ¹, P. Nandy ¹, S. K. Biswas ¹, P. Chakrabarty ¹

Affiliations
1 Department of Chemistry, Bose Institute, Calcutta-700 009, IN

Abstract

Turbidimetric assay study of agglutination induced by lectin RCA₁, in erythrocyte ghost membranes of several vertebrates and in the liposomes of their polar lipid isolates, demonstrates that glycolipid is one of the major receptors of lectin RCA₁, in all these systems. The study also shows that Mg²⁺, a divalent metal ion enhances this agglutination. whereas the effect of Ca²⁺ is not so significant in majority of the cases. Reversal of agglutination is studied after addition of galactose to the assay mixture.

Keywords

Glycolipid, RCA, Agglutination, Erythrocyte Ghost, Liposome.

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