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Habbu, P. V.
- Antidiarrhoeal Activity of Abutilon indicum Linn Leaf Extract
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PDF Views:666
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Journal of Natural Remedies, Vol 4, No 1 (2004), Pagination: 12-16Abstract
Objective: The present study is aimed to evaluate the leaf extracts of Abutilon indicum for acclaimed anti-diarrhoeal activity using albino rats. Materials and methods: Anti-diarrhoeal activity of Abutilon indicum was evaluated by gastro intestinal motility, castor oil-induced diarrhoea and prostaglandin E2 -induced enteropooling in rats. Loperamide was used as a standard drug. Results : The study revealed that, the methanolic extract and aqueous extract possessed significant antidiarrhoeal activity in castor oil-induced diarrhoea and prostaglandin E2-induced diarrhoea, compared to the control group. Conclusion: Abutilon indicum showed significant anti-diarrhoeal activity as compared to loperamide and can be recommended for further studies.Keywords
Abutilon indicum, Loperamide, Antidiarrhoeal Activity, Albino RatsFull Text
- Isolation, Characterization and Evaluation of Endophytic Fractions of Centella asiatica Linn. (Leaves) for Invitro Antioxidant Activity
Abstract Views :307 |
PDF Views:219
Authors
Affiliations
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
Source
Journal of Natural Remedies, Vol 20, No 1 (2020), Pagination: 29-41Abstract
In the present investigation, we have carried out the isolation of fungal endophytes from Centella asiatica Linn leaves followed by fermentation and extraction of fungal endophytes with non-polar solvents such as chloroform, ethyl acetate and n-butanol. Preliminary phytochemical investigation of endophytic crude fractions of leaves were also determined to detect the presence of primary and secondary metabolites followed by invitro free radical scavenging activity by reducing power, DPPH and hydroxyl radical assay. The chloroform fungal endophytic fractions were subjected to column chromatography by gradient elution technique for isolation of possible secondary metabolite. Reducing power of endophytic extracts of C. asiatica Leaf (CAL-1) (50-450 μg/ml) increased with increase in concentration. Reaction with DPPH radicals of CAL-1 showed good scavenging activity. The IC50 values for Ascorbic acid, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 30.33 μg/ml, 66.58 μg/ml, 79.33 μg/ml and 96.39 μg/ml respectively. In hydroxyl radical assay, The IC50 values for mannitol, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 121.06 μg/ml, 141.21 μg/ml, 181.80 μg/ml and 189.90 μg/ml respectively. The endophytic crude fractions of ethyl acetate exhibited potent antioxidant activity as compared to other fractions. Hence, ethyl acetate fungal endophytic fractions of Centella asiatica Linn leaves can be employed as a potential antioxidant in the prevention of oxidative stress caused by the free radicals.Keywords
Antioxidant, Centella asiatica, Column Chromatography, Endophyte.Full Text
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