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Pumipuntu, Natapol
- Screening Method for Staphylococcus aureus Identification in Subclinical Bovine Mastitis from Dairy Farms
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Authors
Natapol Pumipuntu
1,
Suphang Kulpeanprasit
1,
Sirijan Santajit
1,
Witawat Tunyong
1,
Kong-Ngoen Thida
1,
Woranich Hinthong
2,
Nitaya Indrawattana
1
Affiliations
1 Department of Microbiology and Immunology, Mahidol University, Bangkok, TH
2 HRH Princess Chulabhorn College of Medical Science Bangkok, TH
1 Department of Microbiology and Immunology, Mahidol University, Bangkok, TH
2 HRH Princess Chulabhorn College of Medical Science Bangkok, TH
Source
Veterinary World, Vol 10, No 7 (2017), Pagination: 721-726Abstract
Background: Staphylococcus aureus is one of the most important contagious bacteria causing subclinical bovine mastitis. This bacterial infection is commonly identified by determine the pathogen in bovine milk samples through conventional technique including coagulase test. However, this test has several disadvantages as low sensitivity, risk of biohazard, cost expensive, and limited preparation especially in local area.
Aim: Aim of this study was to compare and assess the screening method, Mannitol fermentation test (Mannitol salt agar [MSA]), and deoxyribonuclease (DNase) test, for S. aureus identification in milk samples.
Materials and Methods: A total of 224 subclinical bovine mastitis milk samples were collected from four provinces of Thailand and determined S. aureus using conventional method and also subjected to the screening test, MSA and DNase test. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) among both tests were analyzed and compared to the tube coagulase test (TCT), as reference method. Immunological test by latex agglutination and molecular assay by determined spa gene were also used to identify and differentiate S. aureus.
Results: A total of 130 staphylococci were isolated by selective media, Gram-stain, and catalase test. The number of S. aureus which identified using TCT, MSA and DNase test were 32, 102, and 74 isolates, respectively. All TCT results were correlated to results of latex agglutination and spa gene which were 32 S. aureus. MSA showed 100% sensitivity, 28.57% specificity, 31.37% PPV, and 100% NPV, whereas DNase showed 53.13% sensitivity, 41.84% specificity, 22.97% PPV, and 73.21% NPV. DNase test showed higher specificity value than MSA but the test presented 26.79% false negative results whereas no false-negative result from MSA when comparing to TCT.
Conclusion: MSA had a tendency to be a good preference for screening S. aureus because of its high sensitivity and NPV. The result from this study will improve a choice to use a screening test to diagnose S. aureus of veterinary field for prompt disease controlling and effective treatment.
Keywords
Bovine Mastitis, Deoxyribonuclease Test, Mannitol Fermentation Test, Screening Methods, Staphylococcus aureus.Full Text
- Cryptosporidiosis:A Zoonotic Disease Concern
Abstract Views :174 |
PDF Views:0
Authors
Affiliations
1 One Health Research Unit, Mahasarakham University, Maha Sarakham, TH
1 One Health Research Unit, Mahasarakham University, Maha Sarakham, TH
Source
Veterinary World, Vol 11, No 5 (2018), Pagination: 681-686Abstract
Cryptosporidiosis is considered to be a crucial zoonotic disease caused by worldwide distributing parasitic protozoa called Cryptosporidium spp. Cryptosporidiosis becomes a major public health and veterinary concern by affecting in human and various host range species of animals. Essentially, its importance of infection is increasing because of the high incidence in young children, immunocompromised persons, or immunodeficiency syndrome patients, especially in HIV/AIDS, and it is also one of the most causes of mortality in those patients who infected with Cryptosporidium spp. as well as young animals. All domestic animal, livestock, wildlife, and human can be potential reservoirs that contribute Cryptosporidium spp. to food and surface waters and transmitted to other hosts through fecal-oral route. The oocyst stage of Cryptosporidium spp. can remain infective and resistant to various environmental exposure and also resistant to many general disinfecting agents including chlorination which normally used in water treatment. Therefore, the understanding of these zoonotic pathogens is very essential in both animal and human health. This review focuses on the biology, life cycle, transmission, diagnosis, treatment, prevention, and control of this protozoan infection to emphasize and remind as the significant One Health problem.Keywords
Cryptosporidiosis, Diarrhea, Waterborne Disease, Zoonosis.Full Text
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