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Maan, Narender Singh
- A Comprehensive Study on Seroprevalence of Bluetongue Virus in Haryana State of India
Abstract Views :246 |
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Materials and Methods: A total of 803 serum samples, 408 of cattle and 395 of buffalo origin, respectively, were collected from different villages of Haryana. Sampling was done randomly to obtain unbiased results. The samples were evaluated by a competitive enzyme-linked immunosorbent assay for the presence of BTV antibodies.
Results: Overall seroprevalence of BTV antibody in cattle and buffaloes for all 21 districts of Haryana state was found to be 75.49% and 92.91%, respectively. The prevalence of BTV in different agroclimatic zones ranged between 72-77% and 90-94% for cattle and buffalo, respectively. In buffaloes, the BTV seroprevalence was comparatively higher than in cattle.
Conclusion: The study showed that BTV is circulating in cattle and buffalo populations in the Northern part of India.
Authors
Sushila Maan
1,
Anuj Tiwari
2,
Deepika Chaudhary
1,
Anita Dalal
1,
Nitish Bansal
1,
Vinay Kumar
1,
Kanisht Batra
1,
Aman Kumar
1,
Naresh Kumar Kakker
3,
Narender Singh Maan
4
Affiliations
1 Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
2 Department of Veterinary Microbiology, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, IN
3 Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
4 Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
1 Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
2 Department of Veterinary Microbiology, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, IN
3 Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
4 Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
Source
Veterinary World, Vol 10, No 12 (2017), Pagination: 1464-1470Abstract
Aim: The aim of present study was to determine seroprevalence of bluetongue virus (BTV) in Haryana state of India.Materials and Methods: A total of 803 serum samples, 408 of cattle and 395 of buffalo origin, respectively, were collected from different villages of Haryana. Sampling was done randomly to obtain unbiased results. The samples were evaluated by a competitive enzyme-linked immunosorbent assay for the presence of BTV antibodies.
Results: Overall seroprevalence of BTV antibody in cattle and buffaloes for all 21 districts of Haryana state was found to be 75.49% and 92.91%, respectively. The prevalence of BTV in different agroclimatic zones ranged between 72-77% and 90-94% for cattle and buffalo, respectively. In buffaloes, the BTV seroprevalence was comparatively higher than in cattle.
Conclusion: The study showed that BTV is circulating in cattle and buffalo populations in the Northern part of India.
Keywords
Bluetongue, Bluetongue Virus, Buffalo, Cattle, Competitive Enzyme-Linked Immunosorbent Assay, Haryana, India, Serology.Full Text
- Molecular Analysis of Genome Segment-3 of Bluetongue Virus Serotype 12 Isolates From Haryana
Abstract Views :151 |
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Materials and Methods: Blood and swab samples were collected from goat and sheep suspected to be suffering of BT from different outbreaks from Gurugram, Sirsa, Hisar, and Karnal districts of Haryana. The samples were grown in insect and mammalian cell lines. After preliminary identification, serotyping was done using BTV type-specific quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Sequencing was performed using terminal and walking internal primers specific for Seg-3 on ABI Capillary Sequencer 3130 using a “BigDye cycle sequencing kit.” The obtained sequence data were analyzed with various bioinformatic tools.
Results: Real-time PCR results confirmed the samples to be positive for BTV-12. The Seg-3 of Indian isolates was most closely related to that of a south Indian isolate of BTV-12 from Andhra Pradesh (KC662614) with 97% nucleotide identity.
Conclusion: The study confirmed the circulation of BTV-12 in Haryana, India. The variations shown in genome Seg-3 of BTV-12 isolates may have some significance and need to be further explored.
Authors
Anita Dalal
1,
Sushila Maan
2,
Nitish Bansal
2,
Vinay Kumar
2,
Aman Kumar
2,
Narender Singh Maan
3,
Naresh Kumar Kakker
1
Affiliations
1 Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
2 Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
3 Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
1 Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
2 Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
3 Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
Source
Veterinary World, Vol 10, No 11 (2017), Pagination: 1389-1393Abstract
Aim: The present study was designed to characterize the genome segment 3 (Seg-3) of bluetongue virus (BTV) serotype 12 isolates from different outbreaks of Bluetongue disease in Haryana, India.Materials and Methods: Blood and swab samples were collected from goat and sheep suspected to be suffering of BT from different outbreaks from Gurugram, Sirsa, Hisar, and Karnal districts of Haryana. The samples were grown in insect and mammalian cell lines. After preliminary identification, serotyping was done using BTV type-specific quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Sequencing was performed using terminal and walking internal primers specific for Seg-3 on ABI Capillary Sequencer 3130 using a “BigDye cycle sequencing kit.” The obtained sequence data were analyzed with various bioinformatic tools.
Results: Real-time PCR results confirmed the samples to be positive for BTV-12. The Seg-3 of Indian isolates was most closely related to that of a south Indian isolate of BTV-12 from Andhra Pradesh (KC662614) with 97% nucleotide identity.
Conclusion: The study confirmed the circulation of BTV-12 in Haryana, India. The variations shown in genome Seg-3 of BTV-12 isolates may have some significance and need to be further explored.
Keywords
Bluetongue, Bluetongue Virus-12, Genome Segment-3, Haryana, Real Time, Serotype, Sequencing.Full Text