Refine your search
Collections
Co-Authors
- S. Jeyarani
- P. Karuppuchamy
- S. Manimegalai
- P. S. Shanmugam
- R. Balagurunathan
- N. G. V. Rao
- R. J. Rabindra
- G. Santharam
- C. Muthiah
- S. Jayaraj
- M. Maheesha
- V. Balasubramani
- M. Murugan
- M. Raveendran
- G. Rajadurai
- T. Tamilnayagan
- E. Kokiladevi
- G. Priyatharsini
- K. Premalatha
- S. V. Krishnamoorthy
- K. Senguttuvan
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sathiah, N.
- In vivo Enhancement of Nucleopolyhedrovirus Infection in Helicoverpa armigera (Hübner) by the Granulovirus of Spodoptera litura Fabricius
Abstract Views :330 |
PDF Views:143
Authors
Affiliations
1 Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
1 Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 26, No 3 (2012), Pagination: 234–239Abstract
Biopesticides based on baculoviruses offer great scope as promising viral insecticides against Helicoverpa armigera (Hübner). However, an important limitation in the use of baculoviruses is their reduced efficacy against grown up larvae. The infectivity of NPV against grown up larvae were reported to be enhanced by the presence of a viral enhancing factor (VEF) in granuloviruses (GV). Hence, we investigated the possible interaction of GVs of Spodoptera litura (SlGV), Agrotis segetum (AsGV), Plutella xylostella (PxGV), Achaea janata (AjGV) and Chilo infuscatellus (CiGV) separately with the NPV of H. armigera (HearNPV) against second, third, fourth and fifth instar H. armigera. Of the GVs tested with HearNPV, SlGV alone synergized HearNPV action with reduced LC50 values. The integration of SlGV with HearNPV resulted in 9.38, 10.93, 10.70 and 13.32 fold reduction in LC50 values against the second, third, fourth and fifth instars respectively. Tests conducted with heat inactivated (at 75, 80, 85 and 121°C for 10 min) and heat shielded SlGV could give similar effects with HearNPV indicating that the cause for the virulence enhancement rests with the capsular protein and not the virions of the SlGV, since the virions could be inactivated at temperatures more than 70°C. Integration of SlGV and HearNPV was found to shorten the LT50 values.Keywords
Helicoverpa armigera, Granulovirus, Nucleopolyhedrovirus, Enhancing Factor.Full Text
References
- Chakraborty M, Narayanan K, Sivaprakash MK. 2003. In vivo enhancement of nucleopolyhedrovirus oforiental armyworm, Mythimna separata using viralenhancing factor of a granulosis virus of Spodopteralitura. In: Proc Natl Symp Bio-Management InsectPests. 29–31 March, 2003, Annamalai University,Annamalainagar, India. pp. 43–44.
- Duncan OD. 1966. Path analysis: Sociological examples.American J Sociol. 72: 1–6.
- Evans HF, Shapiro M. 1997. Viruses. pp 17-54. In: LaceyL. (Ed.). Manual of Techniques in Insect Patholog. Academic Press, London.
- Finney DJ. 1962. Probit Analysis. Cambridge UniversityPress, Cambridge and New York.
- Granados RR. 1990. Baculovirus proteins and viralpesticides containing same. U.S. Patent No. 4: 973,667.
- Gröner A. 1986. Specificity and safety of baculoviruses. pp. 177–202. In: Granados RR, Federici BA. (Eds.),The biology of baculovirus, biological properties andmolecular biology. Boca Raton, FL: CRC Press.
- Hackett KJ, Boore A, Deming C, Buckley E, Camp M,Shapiro M. 2000. Helicoverpa armigera. Granulovirusinterference with progression of H. zea larvae. J Invertebr Pathol. 75: 99–106.
- Hukhara T, Tamura K, Zhu Y, Abe H, Tanada Y. 1987. Synergistic factor shows specificity in enhancingnuclear polyhedrosis virus infections. Appl EntomolZool. 22: 235–236.
- Jeyarani S, Karuppuchamy P. 2010. Investigations on theenhancing efficacy of granulovirus on nucleopolyhedrovirusof Helicoverpa armigera (Hübner). J Biopest. 3(1): 172–176.
- King ABS. 1994. Heliothis/Helicoverpa (Lepidoptera:Noctuidae). pp. 39–106. In: Matthews GA, Tunstall, J. P. (Eds.), Insect Pests of Cotton. CAB International,UK.
- Lepore LS, Roelvink PR, Granados RR. 1996. Enhancingthe granulosis virus protein that facilitates nucleopolyhedrovirus(NPV) infections, is a metalloprotease. J Invertebr Pathol. 68: 131–140.
- Ping L, Lin MX, Ping XJ, Wen JX, Bai YL, Rong GJ. 1999. Cloning, expression and bioassay of the geneencoding viral enhancing factor of Trichoplusiani granulosis virus. Chinese J Biol Control, 15:188–189.
- Shanower TG, Romeis J, Minja EM. 1999. Insect pests ofpigeonpea and their management. Ann Rev Entomol. 44: 77–96.
- Shapiro M. 2000. Effect of two granulosis viruses on theactivity of the gypsy moth (Lepidoptera: Lymantriidae)nuclear polyhedrosis virus. J Econ Entomol. 93:1633–1637.
- Sharma HC. 2005. Heliothis/Helicoverpa Management:pp: 469. Emerging Trends and Strategies for FutureResearch. Oxford and IBH Publishers, New Delhi,India.
- Shorey HH, Hale RL. 1965. Mass rearing of the larvae ofnine noctuid species on a simple artificial medium. J Econ Entomol. 58: 522–524.
- Tanada Y. 1959. Synergism between two viruses of thearmyworm, Pseudalatia unipuncta (Haworth)(Lepidoptera : Noctuidae). J Invertebr Pathol. 1:215–231.
- Tanada Y. 1985. A synopsis of studies on the synergisticproperty of an insect baculovirus. A tribute to EdwardA. Steinhaus (Founders Lecture). J Invertebr Pathol. 45: 125–138.
- Safety of Uv-selected Helicoverpa armigera Nucleopolyhedrovirus to Non-target Beneficial Organisms
Abstract Views :225 |
PDF Views:116
Authors
Affiliations
1 Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, IN
1 Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 107-112Abstract
The safety of the UV-selected Coimbatore isolate of Helicoverpa armigera nucleopolyhedrovirus (HaNPV-UVT-CBEI) was tested against the non-target organisms viz., Trichogramma chilonis, Chrysoperla carnea, honey bee species and Bombyx mori to find out whether the cyclical exposure of HaNPV to ultra-violet (UV) radiation could cause adverse effect on the above organisms. The results showed that the HaNPV-UVT-CBEI had no adverse effect on the growth parameters, survival and production parameters of the organisms tested in comparison with the UV shielded HaNPV (HaNPV-UVS-CBEI). The cyclical exposure of HaNPV to UV radiation for the selection of UV tolerant strain did not have any deleterious effect on the safety of the virus to the non-target organisms.Keywords
Apis sp., Bombyx mori, Chrysoperla carnea, Helicoverpa armigera, Nucleopolyhedrovirus, Safety, Trichogramma chilonis.Full Text
- Susceptibility of Different Instars of Earias vittella (Fabricius) to Bacillus thuringiensis Insecticidal Proteins
Abstract Views :233 |
PDF Views:138
Authors
Affiliations
1 Department of Agric. Entomology, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, IN
2 Department of Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444104, Maharashtra, IN
1 Department of Agric. Entomology, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, IN
2 Department of Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444104, Maharashtra, IN
Source
Journal of Biological Control, Vol 21, No 2 (2007), Pagination: 217-221Abstract
The susceptibility of different instars of Earias vittella (Fabrieius) to Bacillus thuringiensis (Bt) insecticidal proteins (ICP) was studied. The LC50 of CrylAa, CrylAb, Cry1Ac, CryiC and Cry1E were 108.54, 97.04, 66.62, 1240.83 and 1335.60 ng/cm2, respectively to E. vittella neonates. The order of toxicity of different ICPs to second and third instar was Cry1Ac > CrylAb > CrylAa. Overlap 95 per cent fiducial limits at LC50 of CrylAa and CrylAb showed similar toxicity of these toxins to second and third instars of E. vittella. The possibility of these toxins and toxicity parameters in resistance management is discussed.Keywords
Bacillus thuringiensis, Earias vittella, Insecticidal Proteins, Median Lethal Concentration, Toxicity.Full Text
- Effect of Combinations of Vairimorpha necatrix (Kramer) with Antibiotics on the Susceptibility of Spodoptera litura (Fabricius)
Abstract Views :154 |
PDF Views:108
Authors
Affiliations
1 Department of Agricultural Entomology Tamil NaduAgricultural University Coimbatore 641 003, Tamil Nadu, IN
2 Department of Agricultural Entomology Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
1 Department of Agricultural Entomology Tamil NaduAgricultural University Coimbatore 641 003, Tamil Nadu, IN
2 Department of Agricultural Entomology Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 20, No 1 (2006), Pagination: 97-100Abstract
Vairimorpha necatrix (Kramer) was combined with different antibiotics to increase its pathogenicity. It was administered to third instar larvae of Spodoptera litura (Fabricius) under laboratory conditions by diet surface treatment and the mortality and spore yield was studied. Reproductive spores of V.necatrix at 106 spores/ml were mixed with 100 ppm of antibiotics viz. gentamycin, oxytetracycline, ampicillin, kana mycin and chloramphenicol. The results showed that the larval mortality due to various Vairimorpha antibiotic combinations ranged from 10 to 62.07 per cent and it was high compared to prepupal and pupal stages. Assessment of spore yield in the infected larvae, pre-pupae and pupae revealed that larval stages recorded higher production of spores which ranged from 0.23 to 2.36 x 1010 spores for larva, 2.26 to 5.28 x 109 spores for pre pupa and 0.28 to 2.23 x 109 spores for pupa. Among the various combinations, Vairimorpha-gentamycin combination produced higher mortality and spore yield of V. necatrix.Keywords
Antibiotics, Mortality, Spodoptera litura, Spore Yield, Vairimorpha necatrix.Full Text
- Controlled Droplet Application of Nuclear Polyhedrosis Virus with Adjuvants and UV Protectants for the Control of Heliothis armigera Hbn. on Chickpea
Abstract Views :202 |
PDF Views:123
Authors
Affiliations
1 Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
1 Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
Source
Journal of Biological Control, Vol 3, No 1 (1989), Pagination: 37-39Abstract
In a field experiment, addition of whole milk 20 percent, whole egg homogenate 10 percent, Ranipal 0.5 percent, RobinbIue 0.5 percent and cotton seed oil 5 percent to nuclear polyhedrosis virus (NPV) in ULV sprays effectively controlled the gram pod borer Heliothis armigera (Hbn.) on chickpea, reduced the damage to pods and increased the yield significantly. The virus was applied three times with a controlled droplet applicator. Addition of crude sugar to NPV gave the highest yield; milk, whole egg homogenate and cotton seed oil being equally effective. In two other experiments, the different adjuvants tested could not Improve the efficacy of the virus and prevent the damage and loss in yield since the larval population at the time of spraying consisted mostly of grownup stages.Keywords
NPV, Controlled Droplet Application, Adjuvants, UV Protectants, Heliothis armigera, Chickpea.Full Text
- Characterisation of native Bacillus thuringiensis isolates toxicity to fall armyworm, Spodoptera frugiperda (J.E. Smith)
Abstract Views :180 |
PDF Views:115
Authors
M. Maheesha
1,
V. Balasubramani
2,
M. Murugan
1,
M. Raveendran
3,
G. Rajadurai
3,
T. Tamilnayagan
3,
E. Kokiladevi
3,
N. Sathiah
1
Affiliations
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
2 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003,, IN
3 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
2 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003,, IN
3 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 35, No 3 (2021), Pagination: 171-180Abstract
Toxicity of nine indigenous Bacillus thuringiensis (Bt) isolates collected from Tamil Nadu, India were tested against fall armyworm, Spodoptera frugiperda. At 30 ?g/ml concentration, two Bt isolates viz., T350 and T532 recorded 100 per cent mortality whereas isolates T527 and T532 registered 96 per cent mortality against neonate larvae of S. frugiperda in leaf dip bioassay. SDS PAGE analysis of spore crystal mixture revealed the presence of Cry1 and Cry2 proteins with visible bands at 130 kDa and 65 kDa. PCR screening results showed the presence of cry1 (cry1A, cry1Aa, cry1Ab), cry2 (cry2Aa, cry2Ab) in four isolates and vip3A genes in three isolates but cry9 gene was not present in any of the isolates tested.
Keywords
Bacillus thuringiensis, bioassay, Cry Genes, fall armyworm, PCR, SDS PAGEFull Text
- Beauveria bassiana as an effective IPM component against cotton stem weevil Pempherulus affinis Faust
Abstract Views :296 |
PDF Views:108
Authors
Affiliations
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641 003, Tamil Nadu, IN
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 35, No 3 (2021), Pagination: 205-208Abstract
The Integrated Pest Management (IPM) in reduction of cotton stem weevil in ecofriendly manner is attaining importance in recent days. In present study, three treatments including IPM module 1, IPM module 2 and farmers’ practice were imposed against cotton stem weevil in a field trial. Among the three treatments, IPM module 2 which included basal application of FYM 25 t/ha and 250 kg/ha of neem cake, seed treatment with Beauveria bassania @10g/kg of seed, border crop with Hibiscus cannabinus, soil drenching with Imidacloprid 17.80 SL @ 25 g a.i./ha (125 ml/ha) at 15 DAS and placement of cotton stem bits (25 kg/500box/ha) + Hibiscus cannabinus stem bits (25 kg/500box/ha) + Chlorpyriphos dust 1.5 DP (2.5 kg/500box/ha) @ 30 DAS followed by earthing up @ 30 DAS recorded least stem weevil infestation of 13.21% with a yield of 1642.75 kg/ha. It was followed by IPM module 1 (21.78%) and farmers’ practice (33.56%) with yield of 1456.25 kg/ha and 1588.25 kg/ha, respectively. The mean survival of plants was also higher in IPM module 2 (94.28%) followed by farmers’ practice (88.57%) and IPM module 1 (80.00%).
Keywords
Cotton stem weevil, farmers’ practice, IPM module, per cent damage, plant survivalFull Text