Refine your search
Collections
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Gadhia, Mohini
- Induction of Chromosomal Aberrations in Mitotic Chromosomes of Fish Boleophthalmus dussumieri after Exposure in Vivo to Antineoplastics Bleomycin, Mitomycin-c and Doxorubicin
Abstract Views :390 |
PDF Views:109
Authors
Affiliations
1 Department of Biosciences, Veer Narmad South Gujarat University, Surat 395 007, IN
2 Department of Aquatic Biology, Veer Narmad South Gujarat University, Surat 395 007, IN
3 C. G. Bhakta Institute of Biotechnology, Gopal Vidyanagar, Bardoli 394 350
1 Department of Biosciences, Veer Narmad South Gujarat University, Surat 395 007, IN
2 Department of Aquatic Biology, Veer Narmad South Gujarat University, Surat 395 007, IN
3 C. G. Bhakta Institute of Biotechnology, Gopal Vidyanagar, Bardoli 394 350
Source
Indian Journal of Science and Technology, Vol 1, No 7 (2008), Pagination: 1-6Abstract
The mitotic chromosomes from the gills of the fish Boleophthalmus dussumieri were studied for the induction of chromosomal aberrations after in vivo treatments with three separate antineoplastic antibiotics: Bleomycin (radiomimetic drug), Mitomycin-C (bifunctional alkylating agent) and Doxorubicin (intercalating agent). Fishes were directly exposed to these agents via intramuscular injections. Two separate treatment timings, 4 hours and 24 hours were selected for all antineoplastic treatments. The concentration doses of Bleomycin (150µg and 300µg), Mitomycin-C (1 µg, 2 µg and 4 µg) and Doxorubicin (10 µg, 20 µg and 40 µg) were decided on the basis of the body weight of fishes and earlier laboratory findings. The results revealed dose and time dependent increase in the chromosomal aberrations after treatments with all antineoplastic drugs. The type of aberrations observed include chromatid breaks, acentric fragments, dicentric and ring configurations. The findings add to the fact that the fish Boleophthalmus dussumieri is a suitable cytogenetic model for in vivo detection of potential mutagens.Keywords
Boleophthalmus dussumieri, Chromosomes, Bleomycin, Mitomycin-c, Doxorubicin, Clastogenic EffectsFull Text
References
- Alder ID (1980) A review of the coordinated research effort on the comparison of the test systems for the detection of mutagenic effects. Mutat. Res. 74, 77-93.
- Alder ID and Brewen JG (1982) Effects of chemicals on chromosome aberration production in male and female germ cells. In: Chemical mutagens principles and methods for their detection (eds. Serresole F J and Hollaender A), pp 1-35 Plenum Press, New York.
- Alink GM, Fredrix-Wolters EMH, van der Gaag MA, van de Kerkhoff JFJ and Poels CLM (1980) Induction of sister chromatid exchanges in fish exposed to Rhine water. Mutat. Res. 74, 369-374.
- Barker GJ and Rackham BD (1979) The induction of sister chromatid exchanges in cultured fish cells (Ameca splendens) by carcinogenic mutagens. Mutat. Res. 68, 381- 387.
- Benedict WF, Backer MS, Haroun L, Choi E and Ames BN (1977) Mutagenecity of cancer chemotherapeutic agents in salmonella microsome test. Cancer. Res. 37, 2209-2213.
- Black JE, Evans J, Harshbarger and Zeigel R (1982) Epizootic neoplasm in fish from a lake polluted by copper mining wastes. J.Natl. Cancer Inst. 69, 915-926.
- Black JE, Holmes P, Dymerski and Zapisek W (1980) Fish tumor pathology and aromatic hydrocarbon pollution in a Great lakes estuary. In: Hydrocarbons and Halogenated hydrocarbons in the aquatic environment. (eds Afghan B K and Mackay, D), pp 559-565, Plenum Press, New York.
- Brown ER, Hazdra JJ, Keith L, Greenspan I, Kwapinski JBG and Beamer P (1973) Frequency of fish tumors found in a polluted watershed as compared to non-polluted Canadian waters. Cancer Res. 33, 189-198.
- Cebula TA (1986) Genetic and physical modulation of anthracycline induced mutagenesis in Salmonella typhimurium. Environ. Mutagen. 8, 672-675.
- Chen AY and Liu LF (1994) DNA topoisomerases: Essential enzymes and lethal targets. Ann. Rev. Pharmacol. Toxicol. 34, 191-218.
- Dresp JE, Schmidt E and Bauchinger M (1978) The cytogenetic effects of bleomycin on human peripheral lymphocytes in vitro and in vivo. Mutat. Res. 56, 341-353.
- Gadhia PK, Dholakia, AH and Gadhia M (1990) Cadmium nitrate induced chromosomal aberrations in a common carp Cyrprinus carpio. Aquacultura Hungarica. 6, 19-23.
- Kligerman AD (1979) Induction of sister chromatid exchanges in the central mudminnow following in vivo exposure to mutagenic agents. Mutat. Res. 64, 205-217.
- Krishnaja AP and Rege MS (1980) Some observation on the chromosome of certain teleosts using a simple method. Ind. J. Exp. Biol. 18, 268-270.
- Krishnaja A P and Rege M S (1982) Induction of chromosomal aberrations in fish Boleophthalmus dussumieri after exposure in vivo to Mitomycin-C and heavy metals mercury, selenium and chromium. Mutat. Res. 102, 71-82.
- Latt SA (1974) Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by Mitomycin-C. Proc. Nat. Acad. Sci. (U.S.A), 71, 3162-3166.
- Lee IP and Dixon RL (1978) Mutagenecity, carcinogenicity and terratogenecity of procarbazine. Mutat. Res. 55, 1-14.
- Minissi S, Ciccotti E and Rizzoni M (1996) Micronuclei test in erythrocytes of Barbus plebejus (Teleostei, pisces) from two natural environments, a bioassay for the in situ detection of mutagens in fresh water. Mutat. Res. 367, 245-251.
- Mitchell S and Kennedy S (1992) Tissue concentrations of organochlorine compound in common seals from the coasts of Northern Ireland. Sci. Total Environ. 115, 235-240.
- Natrajan AT and Ahmstorm G (1969) Heterochromatin and chromosome aberrations. Chromosoma. 28, 48-61.
- Natrajan AT and Raposa T (1975) Heterochromatin and chromosome aberrations: A comparative study of three mouse cell lines with karyotype and heterochromatin distribution. Heredita. 80, 83-90.
- Odeigah C and Osaneyinpeju O (1995) Genotoxic effects of two industrial effluents and ethylmethane sulfonate in Clarius lazera. Food Chem. Toxicol. 33, 501-505.
- Ojima Y, Takayama S and Yamamota K (1972) Chromosome preparation from cultured scale epithelium of teleosts fish. Jap. J. Genet. 47, 445-446.
- Ola HM, Habit EL, Sayed-Ahmed MM, Gabry MS, Khalil MF and Osman AM (2000) Modulation of induced cardiocytotoxicity and genotoxicity of Doxorubicin in Rat by LCarnitine. Jr. Egypt. Nat. Cancer Inst. 12(4), 267-274.
- Park E, Lee J, YI A and Etoh H (1993) Fish cell line (ULF-23HU) derived from the fin of the central mudminnow (Umbra limi) suitable characteristics for clastogenecity assay. In Vitro Cell Develop. Biol. 25, 987-994.
- Paul PWB and Gontzwaard JH (1980) Bleomycin induced structural chromosome aberrations in spermatogonia and bone marrow cells of mice. Mutat. Res. 69, 319-324.
- Prein AE, Thie GM, Alink GM and Koeman JH (1978) Cytogenetic changes in the fish exposed to waters of river Rhine. Sci. Tot. Environ. 9, 287-291.
- Shah VC (1975) Effects of some antibiotics on cell cycles of cultured and meristemic cells. In: Regulation of growth and differentiated functions in eukaryotic cells. (ed. Talwar GP), pp 53-69, Raven Press. New York.
- Shah VC, Rao SRV and Arora OP (1972) Effects of Mitomycin-C on ischolar_main meristem cells of Vicia faba L. II. Induced chromosomal aberrations in relation to cell cycle. Ind. J. Exp. Biol. 10, 431-35.
- Singh B and Gupta RS (1983) Mutagenic responses of thirteen anticancer drugs on mutation induction at multiple genetic loci and sister chromatid exchanges in Chinese hamster ovary cells. Cancer Res. 43, 577-584.
- Sognier MA, Hittelman WN and Pollar M (1982) The relationship between DNA and chromosome damage after bleomycin treatment: Dose response measurements. Mutat. Res. 93, 149-159.
- Yu Y, Zu Z and Hsic AW (1994) Adriamycin induces large deletions as major type of mutation in CHO cells. Mutat. Res. 325, 91-98.
- Static Bioassay and Behavioural Responses of Cypermethrin on Freshwater Teleost, Labeo rohita
Abstract Views :184 |
PDF Views:1
Authors
Affiliations
1 Department of Aquatic Biology, Veer Narmad South Gujarat University, Surat (Gujarat), IN
1 Department of Aquatic Biology, Veer Narmad South Gujarat University, Surat (Gujarat), IN
