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Hussein, Mohammed A.
- Isolation and Identification of Avian Influenza Virus Sub type H9N2 in Poultry from Karbala City, Iraq
Authors
1 Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Kerbala, Karbala, IQ
2 Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, Baghdad, IQ
Source
Research Journal of Pharmacy and Technology, Vol 12, No 4 (2019), Pagination: 1919-1921Abstract
Avian Influenza (AI) disease is an infectious and highly contagious viral disease infected poultry and cause respiratory, digestive distress and sudden death combined with high mortality may arrived to (90%) in infected field. In the last years, poultry industry in Iraq suffered from high mortality arrived to (20-90%) in some farms or more than with severe respiratory distress and sudden death. In addition, grossly characterized by tracheitis, lung congestion, hemorrhagic Payer΄s patches and cecal tonsils that led to high economic losses in poultry industry. This study was designed to isolate and identificate the causative agent by using HA-HI test and RT-PCR assays. A total of 20 samples (lung, trachea, spleen and intestine) were collected from organized farms from Karbala city suspected with AIV infection. All samples were inoculated into the allantoic cavity at (9-11) days old embryonated eggs with cultivation for six days. After that, the allantoic fluid was collected and stored in deep freeze. The HA-HI test was conducted to identify AI virus by using specific antisera. Results revealed that six samples were infected with AIV with HA-HI test and all samples were confirmed by using molecular methods (RT-PCR) test. Nucleotide sequences of H9N2 subtypes of AIV was obtained from NCBI websites and alignment with other researchers concerned in this topic. In conclusion: our results were obtained that all six farms infected with H9N2 subtypes.
Keywords
AIV, RT-PCR, HA-HI Test.References
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- Isolation and Identification of Avian Newcastle Disease in Poultry from Karbala City, Iraq
Authors
1 Department of Pathology and Poultry Disease, University of Kerbala, IQ
2 Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, IQ
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2229-2232Abstract
Newcastle diseases (ND) it is viral disease infected birds and it is infectious, highly contagious diseases and cause respiratory and digestive distress with neurological signs and sudden death combined with high mortality and morbidity may arrived to (93%) in field infection. In Iraq, poultry industry suffered from high mortality arrived to (90%) or more with severe respiratory distress with greenish diarrhea, neurological signs and sudden death, grossly characterized by tracheitis, with hemorrhage of Payer΄s patches, cecal tonsils and proventriculus that lead to high economic losses in poultry industry, so that the aim of this study were isolated NDV with identification by using HA-HI test and RT-PCR assays with specific designing primer. A total of 65 samples (lung, treache, spleen and intestine) were collected from organized farms in karbala city, suspected infected with NDV and after processing these samples were inoculated in to the allantoic cavity of (9-11) days old embryonated eggs with cultivation for five days then collected allantoic fluid and stored in deep freeze. The HA-HI test was conducted to identify NDV by using private antisera, twenty one samples from sixty five are infected with NDV. The molecular methods (RT-PCR) test also confirmed twenty one samples infected with NDV, Nucleotide sequences of NDV was obtained from NCBI websites and alignment with other researchers concerned in this topic.Keywords
NDV, RT-PCR and HA-HI Test.References
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