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Nimmakayala Sridhar , Bondada V. S. S. Surya Kiran , Mithun Rudrapal

Abstract

Based on the ethnobotanical importance as diuretics Aerva lanata and Ecbolium ligustrinum were selected for present screening. Experiment was carried out on male wister strain albino rats using furosemide as standard drug. 20mg/kg and 200mg/kg oral doses are selected for standard and test respectively. For quantifying the diuretic activity the urine output, urine pH, sodium, potassium and chloride levels in the urine are measured. The diuretic index value of Ecbolium ligustrinum is 1.70 and nearer to the standard furosemide value 1.80. Aerva lanata group shows less diuretic index of 1.49 compared to standard. The ratio of the concentration of sodium ions to the potassium ions in control group was found to be 1.39 and the ratio for standard, Aerva lanata and Ecbolium ligustrinum are 1.78, 1.49 and 1.68 respectively. Ecbolium ligustrinum affected the amount of urine excreted and also the electrolyte concentration in urine. The present study concluded that ischolar_mains of Aerva lanata and Ecbolium ligustrinum are having diuretic nature.

Keywords

Diuretic Activity, Aerva lanata, Ecbolium ligustrinum, Roots.

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Rupanjali Sharma ¹, Amrit Goswami ², Mithun Rudrapal ¹, Dipsikha Sharma ¹, Hemanta Kumar Sharma ¹, Dipak Chetia ¹

Affiliations
1 Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786 004, IN
2 CSIR-North East Institute of Science and Technology, Jorhat 785 006, IN

Abstract

A simple Schiff base, N-(pyridine-4-yl-methylene) quinuclidine-3-amine was synthesized from 3-amino-quinuclidine and 4-pyridine carboxaldehyde. The physico-chemical properties of the synthesized com-pound were studied. Molecular docking study was carried out and the quinuclidine derivative was evaluated for its in vitro antimalarial activity against chloroquine-sensitive Plasmodium falciparum strain. Although higher dose of synthesized compound was required for antimalarial activity (EC₅₀ = 13.125 μg/ml) in comparison to chloroquine (EC₅₀ 5.144 μg/ml), the correlation coefficient confirmed good fit of the data. Furthermore, the result of the molecular docking provided insights into the ligand-protein interactions responsible for the inhibitory potency.

Keywords

Antimalarial, Docking, HPLC, Plasmodium falcipurum, Quinuclidine, Mass, NMR.

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