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Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

Two spectrophotometric methods are described for determination of Clarithromycin, Clindamycin and Cefixime in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbance at (666nm for clindamycin and cefixime or 678 nm for clarithromycin) and 510 nm respectively. The amount of bromine reacted corresponds to the amount of each drug. The effect's of acidity, bromate-bromide volume and time, on the absorption were studied. Calibration curves were linear over ranges of 3.2-16 μg.ml^-1 for Clarithromycin,1.6- 4.8 μg.ml^-1 for Clindamycin, 0.8-7.2 μg.ml^-1 for Cefixime in case of methylene blue and of 6.4-19.2 μg.ml^-1 for Clarithromycin, 0.8-4.0μg.ml^-1 for Clindamycin, 0.4-2 μg.ml^-1 for Cefixime in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Clarithromycin, Clindamycin, Cefixime, Methylene Blue and Methyl Orange.

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Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

An isocratic HPLC method had been developed for rapid simultaneous determinathion of three cephalosporines including cefepime HCl, cefoperazone, and cefotaxime Na in pure form or in pharmaceutical dosage form within less than 15 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 7.8 using triethyalamine (18:82 v/v) at ambient temperature. The flow rate was 1 ml/min and maximum absorption was measured at 254 nm.

Keywords

Cefepime, Cfotaxime, Cefoperazone , Phosphate Buffer and HPLC.

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Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

Two spectrophotometric methods are described for determination of Clarithromycin and Clindamycin in bulk and pharmaceutical dosage forms using two acidic dyes, bromocresol purple and Bromocresol green to form ion pair extractable complexes with clarithromycin and clindamycin HCl then measuring absorbances at (390nm for clarithromycin or 397 nm for clindamycin) and 413 nm respectively. The effect,s of acidity, buffer volume and dye concentration, on the absorption were studied. Calibration curves were linear over ranges of 12-28 μg.ml-1 for Clarithromycin and 8- 40 μg.ml-1 for Clindamycin in case of bromocresol purple and of 4-20 μg.ml-1 for Clarithromycin and 16-32μg.ml-1 for Clindamycin in case of Bromocresol green. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Clarithromycin, Clindamycin, Bromocresol Purple and Bromocresol Green.

Full Text

     

Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ²

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG
2 Department of Medicinal Chemistry, Zagazige University, Zagazig, IN

Abstract

An quantitative spectrophotometric method for determination of cefotaxime Na and cefixime. The developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with DTNB as a chromogenic reagent which could be used for their analysis in pure forms and in pharmaceutical formulations then measuring absorbances at (417nm for cefotaxime and cefixime at 411 nm). The concentration of NaOH, DTNB concentration and hydrolysis time were studied. Calibration curves were linear over ranges of 0.8-4 μg.ml^-1 for Cefotaxime and 0.4- 2 μg.ml^-1 for Cefixime. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Cefotaxime, Cefixime, NaOH, DTNB.

Full Text

     

Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

An isocratic HPLC method had been developed for rapid simultaneous separation and determination of two cephalosporins with one macrolide including Claritromycin, Cefixime and Cefoperazone in pure form or in pharmaceutical formulations within less than 5 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase in addition to flow rates was studied. Beer's law was obeyed in the range of 1-50 μg/ml for Clarithromycin and Cefixime or 2-50 μg\ml for Cefoperazone. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and were validated when obtained results were compared with reference methods.

Keywords

Clarithromycin, Cefixime, Cefoperazone, Phosphate Buffer and HPLC.

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