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Shah, Mamta
- A Pharmacognostical study on Fumaria parviflora Lamk.
Authors
1 L. M. College of pharmacy, Ahmedabad, IN
Source
Journal of Natural Remedies, Vol 16, No 1 (2016), Pagination: 1-6Abstract
Fumaria parviflora Lamk., is a valued herb in Ayurvedic medicine and is used as Parpata by majority of Ayurvedic practitioners amongst the other plant sources mentioned under the same common name. It is found in many parts of India from Indo-Gangetic plain and Nepal down to the Nilgiri Mountains. The whole plant is diuretic, diaphoretic, aperient, laxative and anthelmintic. It is used as antipyretic, blood purifier and in skin disorders. In the present study, physico-chemical parameters were established for identification of the drug. Protopine and β-sitosterol were quantified by validated HPTLC method, developed using precoated silica gel plates as a stationary phase and toluene: ethyl acetate: diethyl amine (7: 2: 1) and toluene: methanol (9.4: 0.6) as a mobile phase respectively. It is a diffuse, annual herb with thin winged stem; alternate leaf finely divided into small, linear lanceolate segments, small white or pink flowers with purplish tips. Microscopically ischolar_main can be characterized by the presence of centrally located diarch primary xylem encircled by wide secondary xylem occupying major area and a narrow cork; stem by collenchymatous hypodermis, vascular bundle capped with lignified pericyclic fibres and hollow pith; leaf by vascular bundles with groups of sclerenchyma underneath the phloem and narrow spongy parenchymatous lamina. Powder can be typified by xylem vessels with varied thickening, lignified and thick walled testa and spherical pollen grains. The plant was found to be rich in alkaloids. The amount of protopine and β-sitosterol were found to be 0.47 – 0.50% w/w and 0.23 – 0.26% w/w. The quality parameters and HPTLC method developed would serve as useful gauge in standardization of Fumaria parviflora.
Keywords
Fumaria parviflora, HPTLC, Protopine, β-SitosterolFull Text
- Correlation of Serological and Molecular Methods in Hepatitis B & C Reactive Blood Donors
Authors
1 Dept. of Immunohematology and Blood Transfusion (IHBT), B.J. Medical college, B2 ward (Blood Bank), Dept. of IHBT, Civil Hospital, Asarwa, Ahmedabad, Gujarat, IN
2 Dept. of IHBT, B.J. Medical College, Ahmedabad, IN
3 Dept of IHBT, B.J. Medical College, Ahmedabad, IN
4 ICON Hospital, Ahmedabad, IN
Source
The Indian Practitioner, Vol 73, No 12 (2020), Pagination: 35-37Abstract
Background: The Standards for Blood Banks & Blood Transfusion Services, issued by NACO mention that all collected blood units should be tested for the mandatory five tests before issuing to any patient. The choice of method may depend upon the number of units collected by the blood centre, availability of trained staff and equipment apart from cost feasibility. The aim of this study was to determine the frequency and viral load of HBV DNA and HCV RNA in Hepatitis B & C reactive donors respectively, and hence it was intended to contribute in determining whether routine HBsAg and HCV screening of blood donors, using ELISA method alone, provide any concrete benefits with regard to HBV and HCV risk reduction.
Material & Methods: A total of 20,917 blood donors were screened for HBV, HCV, HIV, Syphilis and Malarial parasite as part of routine blood donation screening at a tertiary care teaching hospital blood centre in Western India. 110 donors having reactive report for HBV and 11 donors having reactive report for HCV were used for the present study.
Results: 110 donors were found to be reactive for HBsAg by ELISA testing. Out of these 110 reactive HBsAg donors, only 72 (65%) showed positive results in HBV DNA test. 11 tested anti-HCV positive in ELISA. Out of 11, only 7 (63%) showed positive results in HCV RNA test.
Conclusion: In the present study, approximately 35% of the screened units which were reactive for ELISA were negative for viral load. The factors like deterioration of sample, low viral load, false positive ELISA results may account for the same.
Keywords
Blood Donors, Hepatitis B, Hepatitis C, Serology, Molecular Markers.
References
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