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Subash Chandra Verma ¹, Rachana Rani ¹, Sukriti Nigam ¹, Dinesh Kumar Agrawala ¹, Pramila Pant ¹, Madan Mohan Padhi ¹, Ramesh Babu Devalla ¹, Chhoten Lal Jain ²

Affiliations
1 Central Council for Research in Ayurveda and Siddha, 61-65, Institutional Area, Opp.-D-Block, Janakpuri, New Delhi-110058, IN
2 Department of Chemistry, M. M. H. College, Ghaziabad, U.P., IN

Abstract

Quality assurance of herbal products may be ensured by proper quality control of the herbal ingredients. In this regard, a rapid, precise, selective, and accurate high performance thin layer chromatographic method of analysis for the determination of ferulic acid in Hemidesmus indicus ischolar_mains extract was developed and validated. The method employed, TLC aluminium plates pre-coated with silica gel 60 F₂₅₄ as the stationary phase. The mobile phase consisted of Toluene: Ethyl acetate: Formic acid; 5:5:0.2 (v/v/v). This method was found to give compact spots for ferulic acid (R_f value of 0.59 ± 0.01). Densitometric scanning of ferulic acid was carried out at λ366 nm in the absorbance/reflection mode. The linear regression analysis data for the calibration plots showed good linear relationship (r²) = 0.999 with respect to peak area and in the concentration range of 50-300 ng spot^-1 band. The method was validated for linearity, precision, limit of detection and limit of quantitation, accuracy and recovery. The present study signifies the use of TLC, HPTLC fingerprint profiles for determining the identity, purity and strength of the ferulic acid in the H. indicus ischolar_main and also for fixing standards for H. indicus containing Ayurvedic formulation.

Keywords

Hemidesmus indicus, HPTLC Method, Development and Validation, Ferulic Acid, Quality Control.

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Subash Chandra Verma ¹, Sukriti Nigam ¹, Chhoten Lal Jain ², Pramila Pant ¹, Madan Mohan Padhi ¹, Ramesh Babu Devalla ¹

Affiliations
1 Central Council for Research in Ayurvedic Sciences, 61-56, Institutional Area, Opp.-D-Block, Janakpuri, New Delhi-110058, IN
2 Department of Chemistry, M. M. H. College, Ghaziabad, U.P., IS

Abstract

Simple, precise, economical and eco-friendly MAE-HPTLC method was developed for the extraction and quantitation of ferulic acid in the whole plant of Cynodon dactylon (L.) Pers. Optimization of various parameters which influence the microwave assisted extraction process, like nature of solvent, volume of solvent, microwave energy input and microwave irradiation time were studied. Effect of solvent polarity on MAE process was observed by using range of solvents from non-polar nature to polar nature. Solvent volume (20 mL), extraction time (120 s) and microwave irradiation power (900 W) were found to be most suitable for extraction of ferulic acid. The developed HPTLC method was validated in terms of linearity, precision, limit of detection, limit of quantitation and accuracy. The maximum yield of extract (1.021%, w/w) from raw plant powder was obtained when extracted with ethanol, while lowest yield of extract (0.421%, w/w) was found with n-hexane. Ethanol extract showed maximum yield of ferulic acid (261.49 μg per 100 gm of dry weight of whole plant), whereas ferulic acid was not detected in n-hexane and dichloromethane extracts.

Keywords

Cynodon dactylon (L.) Pers., Ferulic Acid, MAE-HPTLC Method, Validation.

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