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Pugalenthi, M.
- Antibacterial Activity of Canavalia ensiformis L.DC seeds due to solvent extract and fraction method
Authors
1 Department of Botany, Government Arts College; 1Department of Biotechnology, Karpagam Univ., Coimbatore, IN
Source
Indian Journal of Science and Technology, Vol 3, No 1 (2010), Pagination: 94-95Abstract
The study was performed to evaluate the antibacterial activity of Canavalia ensiformis L.DC. seeds, against clinically important pathogenic bacteria: S. aureus, B. subtilis, S. pyrogens, E. coli, S. typhi, P. vulgaris, P. aeruginosa and K. pneumonia using methanol extract and its fractions in water: chloroform. The methanol extract exhibited better inhibitory activity (MIC < or = 0.125 to 4.0 mg/L) against many test bacteria compared to the extract fractions.Keywords
Canavalia ensiformis, Methanol Extract, Antimicrobial Activity, MICFull Text
References
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- Analysis of Toxic/Antinutritional Constituents and in Vitro Protein Digestibility of Eleven Accessions of Velvet Bean : an Under-Utilized Food Legume
Authors
1 Department of Biotechnology, Karpagam Arts and Science College, Coimbatore - 641 021, Tamil Nadu, IN
Source
The Indian Journal of Nutrition and Dietetics, Vol 43, No 8 (2006), Pagination: 350-356Abstract
Although the common legume seeds have been playing a key role as a protein food in most of the developing countries, their production is not enough to meet the requirements of teeming human population and dwindling livestock industries. Hence, there is a need for exploitation of hitherto neglected under-utilized legumes, whose potential not yet optimally utilized, in view of the pressure on the conventional legumes arising due to increasing multiple uses.
- Nutritional and Antinutritional Properties of a Little Known Pulse, Sesbaniabispinosa
Authors
1 Department of Botany, Government Arts College, Coimbatore-641018, Tamil Nadu, IN
2 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvayalkuppam, Thiruvallur-602025, Tamil Nadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 4 (2012), Pagination: 143-146Abstract
The proximate composition of Sesbaniabispinosareveals that it contains 31.08% crude protein, 6.23% crude lipid,6.81% total dietary fibre, 3.27% ash, 52.61% NFE (Nitrogen FreeExtractives) and 1632Kj 100g-1 DM of calorific value. The mineralcomposition reveals that it is a rich source of potassium, iron, andzinc. The amino acid profiles of the seed protein show that theessential amino acids, sulphur containing amino acids, threonine andisoleucine alone are deficient when compared to FAO/WHO (1991)reference pattern. Fatty acid composition of seed lipids indicate thatlinoleic acid is the most predominant fatty acid. In general the unsaturated fatty acids occur more in level when compared tosaturated fatty acids. The antinutritient profile reveals the occurrenceof low levels of free phenolics and tannins. Though haemmaglutanins (lectins) are detected they can easily be eliminated by cooking process.
Keywords
Underutilized Legume, Nutrient and Antinutrient Composition, In Vitro Protein Digestability, Proximate and Mineral Composition, Calorific Value, Essential Amino Acids.- Effect of Differential Processing on Elimination of Oligosaccharides in Canavalia Beans
Authors
1 Department of Botany, Govt Arts College, Coimbatore, IN
2 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvayalkuppam, Tiruvallur-602025, Tamilnadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 119-124Abstract
The present study reveals the presence of substantial levels of oligosaccharides, verbascose, stachyose and raffinose,in all the investigated seven germplasm seed materials of the tribal pulses, Canavalia ensiformis DC and Canavalia gladiata (Jacq.). The oligosaccharides are implicated in flatus production. Therefore the differential processing methods like soaking followed by cooking and treatment with crude α – galactosidase (enzyme) are undertaken. Between these two processing methods, treatment with crude enzyme (α – galactosidase) appears to be more effective in significant reduction in levels of oligosaccharides, raffinose ranging from 77.94% to 82.72%, stachyose ranging from 67.57% to 73.56% and verbascose ranging from 78.67% to 81.40% .
Keywords
Germplasm, Antinutritional Factors, Oligosaccharides, α-Galactosidase, Soaking and Cooking.- Effect of Differential Processing Methods on Elimination of Oligosaccharides in an Underutilized Food and Feed Source, Mucuna Beans
Authors
1 Department of Botany, Government Arts College, Coimbatore - 641 018, Tamil Nadu, IN
2 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranavayalkuppam, Thiruvallur-602025, Tamil Nadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 125-129Abstract
Legumes (pulses) contain a wide array of antinutritional factors (ANFs) associated with their nutrients. Suitable scientific and technological processing methods are needed for the elimination of ANFs without affecting the nutritional potential of the pulses. In the present study the selected underutilized pulses [Mucuna monosperma DC wall ex and Mucuna pruriens var. utilis. (Dc wall ex Wight) (Baker ex Burck)] were subjected to differential processing methods to assess their effectiveness in eliminating oligosaccharides which cause flatus in consuming humans. Between the two treatments (soaking followed by cooking and crude α-galactosidase treatment), the crude α-galactosidase enzyme treatment is found to be more effective in eliminating significant levels of oligosaccharides (70-90%).
Keywords
Western Ghats, Underutilized Pulses, Antinutritional Factors, Oligosaccharides, Crude Α-Galactosidase.- Evaluation of Nutrient and Anti-Nutrient Characteristics of Different Germplasm of Pongamia Pinnata
Authors
1 Department of Botany, Government Arts College, Coimbatore-641 018, Tamilnadu, IN
2 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyalkuppam, Tiruvallur-602 025, Tamilnadu, IN
3 Department of biotechnology, Prathyusha Institute of Technology and Management, Aranvoyalkuppam, Tiruvallur-602025, Tamilnadu, IN
4 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyalkuppam, Tiruvallur-602 205, Tamilnadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 130-135Abstract
Two different germplasm of Pongamia pinnata collected from Omalur, Salem, Tamilnadu and Bharathiar University campus, Coimbatore, Tamilnadu were analyzed for their proximate composition, minerals, seed protein fractionation, amino acid profiles, fatty acid composition of lipids and anti-nutritional substances. Crude protein value was 22.36 g 100 g-1 DM (Omalur germplasm) and 23.69 g 100 g-1 DM (Bharathiar university campus germplasm). The other major nutrient contents (g 100 g-1) were crude lipid 31.06 and 32.16; ash 3.67 and 4.16, total dietary fiber 8.15 and 6.80 and total crude carbohydrate content (NFE) 34.76 and 33.19 respectively for Omalur germplasm and Bharathiar university campus germplasm. The seeds were a rich source of minerals such as K, Ca,Mg and P. The amino acids valine, isoleucine, leucine, histidine, lysine were fairly high when compared with the FAO/WHO amino acid recommended pattern.
The lipids contained high amounts of unsaturated fatty acids in which linoleic acid was the major fatty acid. The in vitro prote digestibility was found to be 72.34% and 72.16% respectively for Omalur and Bharathiar University Campus germplasm. Anti-nutritional substances such as total free phenols, tannins, L-DOPA and haemagglutinating activity, were also analyzed.
Keywords
Proximate Composition, Mineral Composition, Amino Acid Profiles, Fatty Acid Composition, Anti-Nutritional Factors, Germplasm.- Nutritional Assessment and Chemical Composition of Two Germplasm of Mucuna Monosperma DC. ex Wight
Authors
1 Department of Botany, Government Arts College, Coimbatore - 641 018, Tamil Nadu, IN
2 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyal Kuppam, Tiruvallur – 602 025, Tamil Nadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 136-140Abstract
Two germplasm of the tribal pulse Mucuna monosperma DC. ex Wight viz. Yellur, Tirunelveli District, Tamil Nadu (Western Ghats) and Vaitheki falls, Coimbatore District, Tamil Nadu (Western Ghats) were analysed for proximate composition, mineral composition, amino acid composition, In vitro protein digestibility and fatty acid profiles of lipids. Crude proteins, crude lipids, ash, total dietary fibre and NFE constituted 23.10 - 23.45%, 4.36- 4.39%, 3.21- 3.80%, 8.9 - 9.21% and 59.53-60.05%,respectively. The contents of essential amino acids are on par with FAO/WHO requirement pattern except sulphur containing amino acids, leucine and lysine. The two germplasm also show high levels of unsaturated fatty acids comparable with other conventional pulses. So, this pulse deserves large scale cultivation as a protein supplement.