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Kusum . ¹, R. Rania ¹, P. K. Verma ¹, N. K. Pankaj ¹, V. Kant ¹, J. Kumar ², A. K. Srivastava ¹

Affiliations
1 Divisions of Pharmacology and Toxicology, SKUAST, R.S. Pura, Jammu, Jammu and Kashmir, IN
2 Veterinary Physiology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST, R.S. Pura, Jammu, Jammu and Kashmir, IN

Abstract

Molybdenum toxicity produces a state of secondary hypocuprosis, resulting into alterations in normal hematological profile. In the present study, ammonium molybdate alone and with copper sulfate (II) pentahydrate (ameliorative agent) was administered orally for 30 consecutive days in healthy goats of group 1 and 2, respectively, to access the effect on the hematological profile on different predetermined days of dosing. Administration of ammonium molybdate alone produced significant decline in the mean values of hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), total erythrocyte count (TEC), and mean corpuscular hemoglobin concentration (MCHC), with a significant increase in neutrophil level and mean corpuscular volume (MCV). However, values of erythrocyte sedimentation rate, mean corpuscular hemoglobin, and differential leukocyte count were not significantly altered. On comparing observations of ameliorative group with the group 1 goats, it is concluded that the ameliorative copper salt has beneficial effects in alleviating the alterations in the values of Hb, PCV, TLC, TEC, MCV, MCHC, and neutrophils.

Keywords

Ammonium molybdate, copper sulfate, goats, hematological profile

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Rajinder Raina ¹, Pawan Kumar Verma ¹, Kusum . ², Vinay Kant ¹

Affiliations
1 Division of Pharmacology and Toxicology, Sher-e-Kashmir University of Agricultural Sciences and Technology – Jammu. R. S. Pura, Jammu- 181102, IN
2 Division of Pharmacology and Toxicology, Sher-e-Kashmir University of Agricultural Sciences and Technology – Jammu. R. S. Pura, Jammu- 181102

Abstract

α-Cypermethrin, a pyrethroid insecticide has a wide use for crop protection and ectoparasitic control in domestic animals. Excessive dermal exposure due to environmental contamination or inadvertent use may result in hazardous effects in man and animals. In present study the various biomarkers for the oxidative stress have been studied in Wister rats. SOD and catalase activities in erythrocytes were significantly decreased after 30 day of dermal exposure although these were initially over expressed. There was significant decrease (p<0.05) in blood GSH level and GPx activities in all the three groups. Enzymatic activity of GST was not significantly affected on any day of the exposure. Significant increase in lipid peroxidation of erythrocyte membrane from 10 days onwards exposure was also observed. The evaluated enzymatic and non-enzymatic components of the cell suggest that dermal exposure of ?-cypermethrin produces oxidative stress in rats.

Keywords

α-cypermethrin, antioxidant system, dermal application, lipid peroxidation

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Kant V. ¹, Verma P. K. ¹, Pankaj N. K. ¹, Kumar J. ², Kusum . ¹, Raina R. ¹, Srivastava A. K. ³

Affiliations
1 Division of Pharmacology and Toxicology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST, R.S. Pura, Jammu, J&K, IN
2 Division of Veterinary Physiology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST, R.S. Pura, Jammu, J&K
3 Director, National Dairy Research Institute, Karnal, Haryana, IN

Abstract

High affinity of the fluoride to the bone causes intoxication of bone marrow resulting into alterations in normal hematological profile. In present study sodium fluoride alone and with aluminium sulphate (ameliorative agent) was administered orally daily for 30 days in healthy goats of group 1 and 2 respectively to access the effect on the hematological profile on different days of exposure. Exposure of sodium fluoride alone produced significant reduction in hemoglobin (Hb), packed cell volume (PCV), total leucocytes count (TLC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) and increase in blood clotting time. However, values of total erythrocyte count (TEC) and differential leucocytes count (DLC) were not significantly changed. On comparing the observations of ameliorative group with the group 1 goats, it is concluded that the use of the ameliorative salt Al₂(SO₄)₃ has beneficial effects on the alterations in values of Hb, PCV , TLC, MCV, MCH and MCHC except blood clotting time produced by the subacute exposure of sodium fluoride in goats.

Keywords

Aluminium sulphate, Goats, Haematological profile, Sodium fluoride

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