Informatics Publishing Limited

K. Mittal ¹, T. Dhingra ¹, A. Upadhyay ¹, R. Mashru ², J. Malik ³, A. Thakkar ¹

Affiliations
1 ISF College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga, 142 001 Punjab, IN
2 Pharmacy Department, G.H. Patel Building, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, IN
3 University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014, IN

Abstract

Analytical results play a vital role in quality control of bulk drugs and pharmaceutical formulations. The main aim of this proposed research work is to study uncertainty estimation for quantitative determination of Galantamine hydrobromide from pharmaceutical formulation using ultraviolet spectrophotometry. The estimation of uncertainty was performed using cause effect (Ishikawa) diagram. Determination of uncertainty components proved to be a good way for experimental model to obtain low contribution of uncertainty to analytical results. This research paper explains identification of uncertainty sources, starting from a clear declaration of measured species, quantification of these uncertainty sources and a combination of these individual sources to estimate standard and expanded uncertainty. Determination of various components of uncertainty is a best method to confirm that results obtained of analytical methods are certain. It is concluded from the present study that uncertainty estimation for assay of Galantamine hydrobromide from pharmaceutical formulation is influenced by sample concentration rather than volumetric flask and sample mass. Thus sample concentration is the major factor to achieve precise results of the analysis.

Keywords

Galantamine Hydrobromide GH), Uncertainty Estimation, Cause Effect (Ishikawa) Diagram, Ultraviolet Spectrophotometry.

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A. Agrawal , K. Mittal , A. R. Thakkar

Abstract

A simple, accurate, economical, fast and reliable method for the determination of lercanidipine (LR) is highly desirable to support formulation screening and quality control. The method was developed for determination of LR in pure drug and tablet dosage form. Best results were shown in terms of linearity, accuracy, precision, LOD and LOQ for pure drug as well as for tablets. UV absorbance was measured at 332 nm. Excellent linearity (correlation coefficient (r²) = 0.9970) found in the concentration range of 7.5-60 μg/ml. The LOD and LOQ were 1.0770 and 3.2638 μg/ml respectively and good recoveries were achieved (95.779%).

Keywords

Lercanidipine, Methanol, Zero Order Spectra, First Derivative Zero Crossing Spectrophotometer.

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