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Murtaza, Imtiyaz
- Comparative Analysis of Digestive Amylase Activity in some Tropical and Temperate Breeds of Mulberry Silkworm, Bombyx mori L.
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Authors
Affiliations
1 Temperate Sericulture Research Institute, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu-181 002, IN
2 Biochemistry and Molecular Biotechnology Laboratory, Division of PHT, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar 190 025, IN
1 Temperate Sericulture Research Institute, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu-181 002, IN
2 Biochemistry and Molecular Biotechnology Laboratory, Division of PHT, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar 190 025, IN
Source
Current Science, Vol 112, No 03 (2017), Pagination: 624-629Abstract
In the current study amylase activity was carried out by analysis of digestive fluid in diapausing and nondiapausing strains of mulberry silkworm, Bombyx mori L. Six different breeds, viz. Pure Mysore, Nistari, NB4D2, SH6, SKAUR6 and SKUAST28 were selected for the study. The average digestive amylase activity was found highest in Nistari during spring (664.82 μg) and summer (993.97 μg) seasons. However, among the bivoltine breeds SKUAST-28 with the average amylase activity during spring (148.47 μg) and summer (144.04 μg) seasons was found to be a superior breed with respect to this parameter. The amylase activity in tropical non-diapausing breeds is higher than that in the bivoltine breeds of silkworm which is responsible for their higher survival rate under unfavourable conditions.Keywords
Amylase Activity, Bombyx mori L., Diapuasing and Non-Diapausing Strains, Maltose, Survival.- Plasmid-Borne Mercury Resistance in Aquatic Escherichia coli
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Authors
Affiliations
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
2 Divison of Post Harvest Technology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar (J&K), IN
3 S.M.V.D. University, Katra, Jammu (J&K), IN
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
2 Divison of Post Harvest Technology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar (J&K), IN
3 S.M.V.D. University, Katra, Jammu (J&K), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 149-152Abstract
Bacteria (E. coli) isolated from different aquatic bodies of India were analyzed for their tolerance to mercury (HgCl2 ). Out of the 30 isolates of E. coli, collected from water samples of four geographically distinct regions and hospital settings in India, 8 strains showed significantly high levels of tolerance to the inorganic form of mercury i.e mercury chloride (HgCl2). All the eight strains revealed the presence of a plasmid of approximately 24kb, and transformation of the isolated plasmids into the mercury-sensitive competent cells of E. coli DH5 rendered the transformants resistant to the same concentration of mercury as wild type-strains.Keywords
Escherichia Coli, Mercury Resistance, Mercuric Reductase (merA) Gene.- A Comparative Study on Biodegradation of Chlorpyrifos by Wild E. coli and Pseudomonas fluorescens Bacterial Isolates Inhabiting Different Ecosystems of Kashmir Valley
Abstract Views :210 |
PDF Views:72
Authors
Imtiyaz Murtaza
1,
Bushra
1,
Sageera Showkat
1,
Shah Ubaid-Ullah
2,
Omi Laila
1,
Sumyra Majid
1,
Neyiaz A. Dar
1,
Mukhtar Ahmad
3,
Girish Sharma
4
Affiliations
1 Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, IN
2 Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Srinagar 190 004, IN
3 RCRQ Laboratory, SKUAST-K, Shalimar Campus, Srinagar 190 025, IN
4 Department of Biotechnology, Amity University, Sector-125, Noida 201 313, IN
1 Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, IN
2 Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Srinagar 190 004, IN
3 RCRQ Laboratory, SKUAST-K, Shalimar Campus, Srinagar 190 025, IN
4 Department of Biotechnology, Amity University, Sector-125, Noida 201 313, IN
Source
Current Science, Vol 115, No 4 (2018), Pagination: 753-758Abstract
Among 1081 naturally occurring wild isolates exam-ined for E. coli and Pseudomonas fluorescens, EC1 (E. coli) from Dal Lake (Srinagar district) and PF1 (P. fluorescens) from soil samples of Ganderbal district showed maximum tolerance (11 mg/ml) towards chlorpyrifos. The high performance liquid chroma-tography (HPLC) based chlorpyrifos (CP) degrada-tion analysis demonstrated that each isolate degraded chlorpyrifos much more efficiently than the reference strain E. coli MTCC-533 used in the current study. The present study suggests that EC1 and PF1 bacteri-al isolates inhabiting different ecosystems, degrade chlorpyrifos efficiently via genetic determinants and OPP enzymatic system and provide strong basis for development of bioremediation strategies in the area.Keywords
Bioremediation, Chlorpyrifos, E. coli, HPLC, Pseudomonas fluorescens, Resistance.References
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