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Sherlin, Herald J.
- Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain
Authors
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 79-82Abstract
Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is requiredKeywords
Stains , Modified Stains.References
- Anderson J. (2011). An introduction to Routine and special staining. Retrieved on August 18, 2014 from. Godwin, A. (2011).
- Histochemical uses of haematoxylin-a review. Retrieved August 18, 2014 From www.arpapress.comHarris, T. J., & McCormick, F. (2010).
- The molecular pathology of cancer. Nat Rev Clin Oncol, 7(5), 251-256.
- Liquid Paraffin as a Rehydrant for Air Dried Buccal Smear
Authors
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1197-1200Abstract
Aim: To assess the efficacy of liquid paraffin as a rehydrant for air dried buccal smear based on the nuclear and cytoplasmic details. Background: Buccal smear is useful for diagnosing Malignancy, Fungal infection, Viral infection and Vesicullobullous dermatoses. The routine practice is to wetfixthe smear and send them to the laboratory for staining and evaluation by a cytopathologist. But drying of smears is inevitable, especially when the aspirate is less and when there is improper fixation. This can cause drying artifacts. An alternative method for overcoming this problem is intentional air drying followed by rehydration. Many rehydrants have been experimented upon. The most common rehydrant being saline. The present study is done to check the efficacy of liquid paraffin as a rehydrant. Material and methods: 2 sets of 20 buccal smears were collected from the patients. One set air dried for 24 hours and the other wet fixed. Conventional pap staining was then carried out. The slides were examined by two observers for preservation of Nuclear and cytoplasmic details based on the semi quantitative scoring system. Results: Excellent nuclear details were seen in 45% of air dried smear compared to 25%in routine wet fixation. The cytoplasmic details of air dried smear and routine wet fixed smear was the same. Conclusion: Liquid paraffin as a rehydrant has shown promising results and the nuclear details were found to marginally better than in routine wet fixed smear. Air dried buccal smears can be used in routine practice especially in rural areas where there is limited access to laboratories and for mass screening.Keywords
Buccal smear, Rehydration, Liquid Paraffin.References
- Hoda R.S., Hoda S.A. Artifacts, contaminants and incidental findings. In: Fundamentals of Pap Test Cytology. Humana Press 2007; 179-186.
- Lencioni LJ, Staffieri JJ, Cardinnet LJ. Vaginal and urinary sediment smear staining technique without previous fixation; adapted to Papanicolaou’s and Shorr’s staining methods. J Lab Clin Med.1954; 44(4): 595-9.
- Chan JKC, Kung ITM. Rehydration of airdried smears with normal saline: application in fine-needle aspiration cytologic examination. Am J Clin Pathol 1988; 89(1):30-4.
- M. S. Israel, L.F. Young. Use of liquid paraffin in the preservation of pathological specimens. J Clin Path.1978; 31(5):499-500.
- Crothers, Barbara and Henry, Michael and Firat, Pinar and Hamper, Ulrike. Nondiagnostic/Unsatisfactory: The Bethesda System for Reporting Thyroid Cytopathology. 2010.
- Ahmed HG, Tom MA. The consequence of delayed fixation on subsequent preservation of urine cells. Oman Medical Journal. 2011; 26(1):14-8.
- Zare –Mirzaie, K. Kalili-Alam and M. Abolhasani. Rehydration of air-dried cervical smears: An alternative to routine wet fixation. Acta Medica Iranica 2007; 45(5):365-68.
- Dietary Habits Leading to Recurrent Aphthous Ulcers-A Survey
Authors
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Saveetha University, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3479-3482Abstract
Background: Aphthous ulcers are ulcers that form on the oral mucous membranes. They are known as aphthae, aphthous stomatitis and also canker sores. Aphthous ulcers are typically recurrent round or oval sores or ulcers inside the mouth found on the inside of the lips and cheeks or underneath the tongue. Patients often complain about very painful wounds inside their mouth that prevent them from eating food. The present study was designed to identify the dietary factors that trigger occurrence of RAS. Materials and Method: A qualitative survey was conducted through a structured Questionnaire presented to individuals with RAS visiting a Dental college and Hospital in Chennai between July to decemebr 2017. The results were expressed in percentage. Result: The study results revealed that 40% of them had a family history of RAS. 85% were anemic and stress was a precipitating factor in all the individuals (100%). Buccal mucosa (80%) was the most common site involved. 21% had RAS during Menstruation. RAS was triggered by consumption of gluten rich food in 80% of the individuals and spicy food in all the participants (100%). Conclusion: Stress, fatigue, everyday consumption of gluten rich diet, and spicy food trigger the development of RAS. This may be prevented by modifying their dietary habits.Keywords
Aphthous, Diet, Gluten, Anemia, Soft Drinks.References
- Pongissawaranun W, Laohapand PP. Epidemiologic study on recurrent aphthous stomatitis in a Thai dental patient population. Community Dent Oral Epidemiol 1991,19:52–3.
- Porter SR, Scully C, Pedersen A. Recurrent aphthous stomatitis. Crit Rev Oral Biol Med 1998, 9:306 –21.
- Barrons RW. Treatment strategies for recurrent oral aphthous ulcers. Am J Health Syst Pharm 2001, 58(1):4150.
- Zhou Y, Chen Q, Meng W, Jiang L, Wang Z, Liu J. Evaluation of penicillin G potassium troches in the treatment of minor recurrent aphthous ulceration in a Chinese cohort: a randomized, double-blinded, placebo and no-treatment-controlled, multicenter clinical trial. Oral Surg Oral Med Oral Pathol Oral Radiol Endod.2010, 109:561–6.
- D, Charon J. Polymorphonuclear neutrophil function in recurrent aphthous stomatitis. J Oral Pathol Med 20: 392-4, 1991.
- Rogers RS, Hutton KP. Screening for hematinic deficiencies in patients with recurrent aphthous stomatitis. Aust J Dermatol 1986, 27:98 –103.
- Olsen JA, Feinberg I, Silverman S. Serum vitaminB12, folate, and iron levels in recurrent oral ulceration. Oral Surg 1982, 54:517–20.
- Nolan A, McIntosh WB, Allam BF. Recurrent aphthousulceration: Vitamin B1, B2 and B6 status and response to replacement therapy. J Oral Path Med 1991, 20: 389–91.
- Ferguson MM, Wray D, Carmichael HA. Coeliac disease associated with recurrent aphthae. Gut 1980;21: 223–6.
- O’Mahony C, O’ Farrelly C, Weir DG. Gluten-sensitive oral ulceration in the absence of coeliac disease. Gut 1985, 26: A1137. 11. Bassel Tarakji, Kusai Baroudi, and Yaser Kharma. The effect of dietary habits on the development of the recurrent aphthous stomatitis:. Niger Med J. 2012 Jan-Mar; 53(1): 9–11.
- Miller MF, Garfunkel AA, Ram C. Inheritence patterns on recurrent aphthous ulcers: Twin and pedigree data. Oral Surg 1977, 43:886 –91.
- McCartan BE, Sullivan A. The association of menstrual cycle, pregnancy, and menopause with recurrent oral aphthous stomatitis: A review and critique. Obstet Gynecol 1992, 80:455– 8.
- Miller MF, Ship II, Ram C. A retrospective study of factors associated with recurrent aphthous ulcers in a professional population. Oral Surg 1977, 43:532–7.
- Besu I, Jankovic L, Magdu IU, Konic-Ristic A, Raskovic S, Juranic Z. Humoral immunity to cow’s milk proteins and gliadin within the etiology of recurrent aphthous ulcers? Oral Dis 2009, 15(8):560-4.
- Porter SR, Scully C, Flint S. Hematologic status in recurrent aphthous stomatitis compared with other oral disease. Oral Surg Oral Med Oral Pathol 1988, 66(1):41-4.
- Grady D, Ernster VL, Stillman L, Greenspan J. Smokeless tobacco use prevents aphthous stomatitis. Oral Surg Oral Med Oral Pathol. 1992, 74:463–65. [PubMed]
- Scheid P, Bohadana A, Martinet Y. Nicotine patches for aphthous ulcers due to Behcet's syndrome. N Engl J Med. 2000, 343: 1816–17. [PubMed]
- P. S. Subiksha: Various remedies for recurrent aphthous ulcer- A review / J. Pharm. Sci. & Res. Vol. 6(6), 2014, 251-253.
- R. Prithi, Sreedevi Dharman: Study on prevalence of recurrent aphthous ulcer among college students. RJPBCS 7(6) Nov-Dec 2016, 1392.
- Tobacco and Betel Nut Chewing Behaviour and its Association with Potentially Malignant Disorders in Chennai
Authors
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Chennai, IN
Source
Indian Journal of Public Health Research & Development, Vol 10, No 11 (2019), Pagination: 130-135Abstract
Background: Betel nut and tobacco chewing habit is considered to be common amongst the individuals of Asian origin. It is associated with oral and oropharyngeal cancer and other potentially malignant disorders (PMD). Attributes like the frequency, duration of chewing, placement of betel nut and tobacco seems to plays a significant role in the initiation of potentially malignant disorders and oral squamous cell carcinoma.
Aim: To assess the Tobacco & Betel nut chewing behaviour& its association with PMDs in chennai population.
Materials and Method: The study group consisted of 20 tobacco & betel nut chewing individuals from sub-urban and rural areas near Chennai with the habit > 1 year. A questionnaire consisting of 16 questions relevant to tobacco and betel nut chewing habits were given and the data was acquired, Clinical examination was done to document the oral findings of the study population associated with PMDs.
Results: There was presence of oral leukoplakia (50%), OSMF (34%) and oral lichen planus (18%) among the study population.
Conclusion: Betel nut and tobacco chewing behaviour and its patterns plays a major role in causing potentially Malignant disorders and it is considered to be one of the major risk factor for the development of Oral squamous cell carcinoma.
Keywords
Tobacco, Betel Nut, Potentially Malignant Disorders, Chewing Habits.- Agar-Paraffin Double Embedding Over Conventional Embedding for Minute Oral Biopsies-Cohort Study
Authors
1 Postgraduate, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
2 Professor and Head of the Department, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
3 Professor, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
4 Reader, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
5 Senior Lecturer, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 14, No 1 (2020), Pagination: 84-88Abstract
Agar-Paraffin double embedding technique is a simple technique that combines the advantages of both the embedding material. It preserves the minute tissue biopsies in orientation and holds them together from getting lost. Oral biopsies, being most commonly small incisional tissues, have to be preserved all through the processing and embedding to ensure optimal visualization of all the mucosal layers without compromise. In the present study, samples were divided into two groups: Group A: Agar-paraffin double embedding (APE) and Group B: conventional paraffin embedding (PE). Tissue samples collected were sectioned into two approximately equal sized bits. Both the bits were simultaneously processed, embedded in two different techniques (APE and PE method). Sections obtained were scored by an observer and analyzed using independent sample t-test (SPSS software version 21) to evaluate the efficacy of agar-paraffin double embedding technique in comparison to the conventional paraffin embedding technique. Agar paraffin embedded tissue was found to be well processed, firm and well preserved. Orientation was comparatively easier and the blocks yielded sections of good quality. They showed no interference with staining and cell morphology was of good clarity. Thus Agar-paraffin embedding technique represents a simple, reliable method that can greatly improve the quality of diagnostic information.Keywords
Agar-Paraffin, Double Embedding, Oral Biopsies.- Knowledge about Legal Aspects of Medical Negligence in India among Dentists–A Questionnaire Survey
Authors
1 III Year MDS, Post Graduate Student, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
2 Professor and Head, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
3 Professor, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
4 Reader, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
5 Senior Lecturer, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN