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Padh, H.
- A New Spectrophotometric Method for the Estimation of Total Alkaloids in the Stem Bark and Seed of Holarrhena antidysenterica (Linn.) Wall. and in the Ayurvedic Formulation, Kutajarishta
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PDF Views:1631
Authors
Source
Journal of Natural Remedies, Vol 2, No 2 (2002), Pagination: 168-172Abstract
Objective: To develop a spectrophotometric method for the estimation of total alkaloids in the stem bark, seed and formulations of Holarrhena antidysenterica. Materials and methods: A spectrophotometric method based on the formation of coloured complex between tropaeolin 'OO' reagent and alkaloids. Results: The developed method shows good accuracy and reproducibility. Conclusion: The method developed is simple, precise and accurate and can be adopted for the routine quality control purposes.Keywords
Holarrhena antidysenterica, Conessine, Spectrophotometric MethodFull Text
- Identification of Single Nucleotide Polymorphism from Indian Bubalus bubalis through Targeted Sequence Capture
Abstract Views :216 |
PDF Views:79
Authors
A. B. Patel
1,
R. B. Subramanian
1,
H. Padh
1,
T. M. Shah
2,
A. Mohapatra
2,
B. Reddy
2,
S. J. Jakhesara
2,
P. G. Koringa
2,
D. Dash
3,
C. G. Joshi
2
Affiliations
1 BRD School of Biosciences, Sardar Patel University, V. V. Nagar 388 120, IN
2 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
3 Roche Diagnostics India Pvt Ltd, Kolkata 700 107, IN
1 BRD School of Biosciences, Sardar Patel University, V. V. Nagar 388 120, IN
2 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
3 Roche Diagnostics India Pvt Ltd, Kolkata 700 107, IN
Source
Current Science, Vol 112, No 06 (2017), Pagination: 1230-1239Abstract
Bubalus bubalis (water buffalo) is an agro-economically important livestock species due to its multipurpose use in India and other Asian countries. The aim of this study was to identify single nucleotide polymorphisms (SNPs) from buffalo genome. Genomic DNA was isolated from 24 blood samples of three Indian buffalo breeds and subjected to targeted pyrosequencing, followed by variant calling and annotation. Target probes for enrichment were designed from exome and 5' and 3' untranslated regions of cattle genome. By targeted pyro-sequencing and variant calling from 3.92 Gb data, 923,964 high-quality SNPs were identified. Many SNPs were identified in regulatory regions, leading to conformational changes in factor-binding sites, which play a role in gene expression as in the case of LPL gene from low-milkproducing samples. Gene ontology (GO) enrichment and clustering, resulted in the enrichment of GO terms involved in milk production and transport, and fertility-related categories. Around 75% of SNPs were located on cattle quantitative trait loci, supporting trait-wise sample collection approach. Further, PCA analysis from the identified SNPs also supported sample selection strategy based on contrasting trait performance.Keywords
Exome, Gene Ontology, Quantitative Trait Locus, Single Nucleotide Polymorphism.Full Text
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