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Narayanaswamy, H. D.
- Sub Chronic Toxicity Study of Rhizopus Oryzae Culture Filtrate from Ground Nut Hay in Rats
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Authors
Affiliations
1 Animal and Fisheries Sciences University, Veterinary College, Hebbal, Bangalore - 560 024, IN
1 Animal and Fisheries Sciences University, Veterinary College, Hebbal, Bangalore - 560 024, IN
Source
Indian Journal of Animal Research, Vol 45, No 2 (2011), Pagination: 130-134Abstract
Sub chronic toxicity study of culture filtrate of Rhizopus oryzae isolated from the fungal contaminated ground nut hay was conducted in rats. The fungi was isolated from the contaminated groundnut hay, which caused mycotoxicosis in cross bred cattle, which exhibited the clinical signs of colic, tenesmus, ruminal atony, anorexia, bleeding from nostrils, rectum and fly bite site. The rats were gavaged with culture filtrate at the dose level of 0.5, 1 and 2 ml daily for 90 days. Clinical signs observed were diarrhea, weakness, slight arching of back and swollen forehead. Loss of balance in the legs region seen. There was a significant increase (P<0.05) in serum concentrations of ALT, AST and creatinine indicated the hepatic and renal damage which were confirmed by histopathology. There were lesions in the GI tract of the treated rats. The present study indicated the toxic feature of the culture filtrate of Rhizopus oryzae isolated from ground nut hay.Keywords
Rhizopus Oryzae, Culture Filtrate, Groundnut Hay, Sub Chronic Toxicity, Rat.
- Efficacy of Diatomacious Earth in Amelioration of Aflatoxin Induced Toxicity in Broiler Chicken
Abstract Views :91 |
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Authors
A. W. Lakkawar
1,
M. L. Sathyanarayana
2,
H. D. Narayanaswamy
1,
Sugunarao
1,
S. Yathiraj
3,
S. K. Isloor
1,
N. B. Shridhar
4,
N. Krishnaveni
1
Affiliations
1 Department of Pathology, Veterinary College, Bengaluru- 560 024, Karnataka, IN
2 Department of Pathology, Veterinary College, Shimoga-577240, Karnataka, IN
3 Department of Pathology, Veterinary College, Bengaluru-560 024, Karnataka, IN
4 Department of Pharmacology and Toxicology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore-560024, Karnataka, IN
1 Department of Pathology, Veterinary College, Bengaluru- 560 024, Karnataka, IN
2 Department of Pathology, Veterinary College, Shimoga-577240, Karnataka, IN
3 Department of Pathology, Veterinary College, Bengaluru-560 024, Karnataka, IN
4 Department of Pharmacology and Toxicology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore-560024, Karnataka, IN
Source
Indian Journal of Animal Research, Vol 50, No 4 (2016), Pagination: 529-536Abstract
The ability of Diatomacious earth (DAE) in reducing the toxic effects of aflatoxin (AF) in broiler diet was evaluated. DAE was supplemented @ 400 and 800 mg kg-1 of feed along with 0.5 and 1 ppm of AF kg-1 of feed. Healthy unsexed day old broiler chicks (n=360) were assigned to 9 groups comprising of control and treatment groups. Feeding of AF resulted in significantly lower feed intake as well as body weight gain and increase in feed conversion ratio in comparison to the control groups. Supplementation of DAE in aflatoxin mixed diet significantly reduced the deleterious effects of AF on growth parameters. Feeding of AF alone caused significant decrease in serum levels of total proteins, albumin, triglycerides and cholesterol. However, significant increase was found in serum levels of AST, ALT, ALP, BUN and creatinine in AF fed broilers. Supplementation of DAE to the AF mixed feed ameliorated the adverse effect of AF on the serum biochemical values by causing increase in serum total proteins, albumin, triglycerides and cholesterol levels. In addition, a significant increase was recorded in AST, ALT, ALP, BUN and creatinine levels in DAE supplemented broilers. The study concluded that diatomaceous earth is an effective adsorbent to decrease the negative effects of aflatoxin in broiler chicken.Keywords
Aflatoxin, Amelioration, Broilers, Diatomaceous Earth, Growth Performance, Serum Biochemistry.References
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- In-Vivo Anti-Inflammatory and Analgesic Screening of Ficus bengalensis Leaf Extract in Rats
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Authors
Prathap Kumar Kothapalli
1,
S. Jagadeesh Sanganal
1,
N. B. Shridhar
1,
H. D. Narayanaswamy
2,
M. Narayanaswamy
3
Affiliations
1 Dept. of Veterinary Pharmacology and Toxicology, Veterinary College, Bangalore-560024, IN
2 Dept. of Veterinary Pathology, Veterinary College, Bangalore-560024, IN
3 Dept. of Veterinary Physiology, Veterinary College, Bangalore-560024, IN
1 Dept. of Veterinary Pharmacology and Toxicology, Veterinary College, Bangalore-560024, IN
2 Dept. of Veterinary Pathology, Veterinary College, Bangalore-560024, IN
3 Dept. of Veterinary Physiology, Veterinary College, Bangalore-560024, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 4, No 4 (2014), Pagination: 174-178Abstract
The present study was conducted to evaluate the analgesic and anti-inflammatory activities of Ficus bengalensis leaf extract in rats using Eddy's hot plate method and formalin induced paw oedema model, respectively. The Ficus benghalensis methanolic leaf extract tested for anti-inflammatory activity, showed a significant (P<0.001) paw volume reduction at dose of 200 mg/kg and at 3 hours. The percent inhibition in paw volume was 65.21% at 200 mg/kg as compared to 62.31% of Diclofenac indicating the anti-inflammatory activity, in formalin-induced paw-edema model. The Ficus benghalensis leaf extract also demonstrated a significant (P<0.001) analgesic activity at both 100 and 200 mg/kg. Thus it can be concluded that the Ficus benghalensis leaf extract possess a potent analgesic and antiinflammatory activities as reflected by the parameters investigated, and demands further research to characterize the novel candidate.Keywords
Analgesic, Anti-Inflammatory, Ficus Bengalensis Leaf Extract, Eddy’s Hot Plate Method and Formalin Induced Paw Oedema Model.- Survivin Expression in Canine Spontaneous Cutaneous and Subcutaneous Tumors and its Prognostic Importance
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Materials and Methods: Forty cases of canine cutaneous and subcutaneous tissue tumors on histopathological examination revealed various round cell, epithelial, and mesenchymal cell tumors. Survivin gene expression was detected in all tumors tested by TaqMan real-time polymerase chain reaction assay by comparative cycle threshold method.
Results: The mean survivin gene expression value of benign tumors was 0.94±0.63 folds and that of malignant tumors was 18.87±5.30 folds. Postsurgical follow up of 30 malignant tumor cases revealed death in 8, recurrence in 7, and neoplastic free alive status in 15 dogs with mean survivin fold difference values of 48.49±12.39, 14.63±6.37, and 5.034±2.27, respectively. The mean survivin gene expression value was significantly higher in malignant (30 cases, 18.87±5.30) compared to benign tumors (10 cases, 0.94±0.63), and it varied between various postsurgical follow-up groups (p<0.05). Survival analysis, using survivin gene expression median cutoff value of 3.74 in 30 malignant tumors, was performed to predict probable survival period in malignant cutaneous and subcutaneous tumors of dogs.
Conclusion: Results of the present study indicated that the expression of survivin in canine cutaneous and subcutaneous tumors has prognostic value, and survivin expression greater than median cutoff value of 3.74 has a poor prognosis.
Authors
N. Kavya
1,
S. Rao
1,
M. L. Sathyanarayana
1,
H. D. Narayanaswamy
1,
S. M. Byregowda
2,
L. Ranganath
3,
A. Kamaran
4,
K. M. Purushotham
2,
T. K. Kishore
1
Affiliations
1 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
2 Department of Biotechnology, Institute of Animal Health and Veterinary Biologicals, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
3 Department of Veterinary Surgery and Radiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
4 Department of Veterinary Medicine, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
1 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
2 Department of Biotechnology, Institute of Animal Health and Veterinary Biologicals, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
3 Department of Veterinary Surgery and Radiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
4 Department of Veterinary Medicine, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
Source
Veterinary World, Vol 10, No 10 (2017), Pagination: 1286-1291Abstract
Aim: The present study was carried out to know the expression level of survivin, an inhibitor of apoptosis protein with an objective to determine its prognostic importance in cutaneous and subcutaneous tissue tumors of dogs.Materials and Methods: Forty cases of canine cutaneous and subcutaneous tissue tumors on histopathological examination revealed various round cell, epithelial, and mesenchymal cell tumors. Survivin gene expression was detected in all tumors tested by TaqMan real-time polymerase chain reaction assay by comparative cycle threshold method.
Results: The mean survivin gene expression value of benign tumors was 0.94±0.63 folds and that of malignant tumors was 18.87±5.30 folds. Postsurgical follow up of 30 malignant tumor cases revealed death in 8, recurrence in 7, and neoplastic free alive status in 15 dogs with mean survivin fold difference values of 48.49±12.39, 14.63±6.37, and 5.034±2.27, respectively. The mean survivin gene expression value was significantly higher in malignant (30 cases, 18.87±5.30) compared to benign tumors (10 cases, 0.94±0.63), and it varied between various postsurgical follow-up groups (p<0.05). Survival analysis, using survivin gene expression median cutoff value of 3.74 in 30 malignant tumors, was performed to predict probable survival period in malignant cutaneous and subcutaneous tumors of dogs.
Conclusion: Results of the present study indicated that the expression of survivin in canine cutaneous and subcutaneous tumors has prognostic value, and survivin expression greater than median cutoff value of 3.74 has a poor prognosis.
Keywords
Cutaneous and Subcutaneous Tumors, Prognostic Value, Quantitative Real-Time Polymerase Chain Reaction, Survivin.Full Text