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Jayaraj, Gifrina
- Liquid Paraffin as a Rehydrant for Air Dried Buccal Smear
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Authors
R. Hannah
1,
Pratibha Ramani
1,
M. P. Brundha
2,
Herald J. Sherlin
1,
Gheena Ranjith
1,
Abilasha Ramasubramanian
1,
Gifrina Jayaraj
1,
K. R. Don
1,
S. Archana
1
Affiliations
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1197-1200Abstract
Aim: To assess the efficacy of liquid paraffin as a rehydrant for air dried buccal smear based on the nuclear and cytoplasmic details. Background: Buccal smear is useful for diagnosing Malignancy, Fungal infection, Viral infection and Vesicullobullous dermatoses. The routine practice is to wetfixthe smear and send them to the laboratory for staining and evaluation by a cytopathologist. But drying of smears is inevitable, especially when the aspirate is less and when there is improper fixation. This can cause drying artifacts. An alternative method for overcoming this problem is intentional air drying followed by rehydration. Many rehydrants have been experimented upon. The most common rehydrant being saline. The present study is done to check the efficacy of liquid paraffin as a rehydrant. Material and methods: 2 sets of 20 buccal smears were collected from the patients. One set air dried for 24 hours and the other wet fixed. Conventional pap staining was then carried out. The slides were examined by two observers for preservation of Nuclear and cytoplasmic details based on the semi quantitative scoring system. Results: Excellent nuclear details were seen in 45% of air dried smear compared to 25%in routine wet fixation. The cytoplasmic details of air dried smear and routine wet fixed smear was the same. Conclusion: Liquid paraffin as a rehydrant has shown promising results and the nuclear details were found to marginally better than in routine wet fixed smear. Air dried buccal smears can be used in routine practice especially in rural areas where there is limited access to laboratories and for mass screening.Keywords
Buccal smear, Rehydration, Liquid Paraffin.
References
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- Dietary Habits Leading to Recurrent Aphthous Ulcers-A Survey
Abstract Views :273 |
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Authors
Affiliations
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Saveetha University, Chennai, IN
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Saveetha University, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3479-3482Abstract
Background: Aphthous ulcers are ulcers that form on the oral mucous membranes. They are known as aphthae, aphthous stomatitis and also canker sores. Aphthous ulcers are typically recurrent round or oval sores or ulcers inside the mouth found on the inside of the lips and cheeks or underneath the tongue. Patients often complain about very painful wounds inside their mouth that prevent them from eating food. The present study was designed to identify the dietary factors that trigger occurrence of RAS. Materials and Method: A qualitative survey was conducted through a structured Questionnaire presented to individuals with RAS visiting a Dental college and Hospital in Chennai between July to decemebr 2017. The results were expressed in percentage. Result: The study results revealed that 40% of them had a family history of RAS. 85% were anemic and stress was a precipitating factor in all the individuals (100%). Buccal mucosa (80%) was the most common site involved. 21% had RAS during Menstruation. RAS was triggered by consumption of gluten rich food in 80% of the individuals and spicy food in all the participants (100%). Conclusion: Stress, fatigue, everyday consumption of gluten rich diet, and spicy food trigger the development of RAS. This may be prevented by modifying their dietary habits.Keywords
Aphthous, Diet, Gluten, Anemia, Soft Drinks.References
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- Agar-Paraffin Double Embedding Over Conventional Embedding for Minute Oral Biopsies-Cohort Study
Abstract Views :462 |
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Authors
Affiliations
1 Postgraduate, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
2 Professor and Head of the Department, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
3 Professor, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
4 Reader, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
5 Senior Lecturer, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
1 Postgraduate, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
2 Professor and Head of the Department, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
3 Professor, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
4 Reader, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
5 Senior Lecturer, Dept. of Oral Pathology, Saveetha Dental College & Hospitals, Saveetha University, IN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 14, No 1 (2020), Pagination: 84-88Abstract
Agar-Paraffin double embedding technique is a simple technique that combines the advantages of both the embedding material. It preserves the minute tissue biopsies in orientation and holds them together from getting lost. Oral biopsies, being most commonly small incisional tissues, have to be preserved all through the processing and embedding to ensure optimal visualization of all the mucosal layers without compromise. In the present study, samples were divided into two groups: Group A: Agar-paraffin double embedding (APE) and Group B: conventional paraffin embedding (PE). Tissue samples collected were sectioned into two approximately equal sized bits. Both the bits were simultaneously processed, embedded in two different techniques (APE and PE method). Sections obtained were scored by an observer and analyzed using independent sample t-test (SPSS software version 21) to evaluate the efficacy of agar-paraffin double embedding technique in comparison to the conventional paraffin embedding technique. Agar paraffin embedded tissue was found to be well processed, firm and well preserved. Orientation was comparatively easier and the blocks yielded sections of good quality. They showed no interference with staining and cell morphology was of good clarity. Thus Agar-paraffin embedding technique represents a simple, reliable method that can greatly improve the quality of diagnostic information.Keywords
Agar-Paraffin, Double Embedding, Oral Biopsies.- Knowledge about Legal Aspects of Medical Negligence in India among Dentists–A Questionnaire Survey
Abstract Views :461 |
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Authors
Affiliations
1 III Year MDS, Post Graduate Student, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
2 Professor and Head, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
3 Professor, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
4 Reader, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
5 Senior Lecturer, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
1 III Year MDS, Post Graduate Student, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
2 Professor and Head, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
3 Professor, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
4 Reader, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN
5 Senior Lecturer, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, SIMATS, Chennai, IN