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Abdollahi, Fatemeh
- Mindfulness-Based Intervention in Relation to Wellness, Emotional Wellbeing and Quality of Life in Breast Cancer Patients
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Authors
Affiliations
1 Department of Psychology, Aligarh Muslim University, Aligarh, Uttar Pradesh, IN
1 Department of Psychology, Aligarh Muslim University, Aligarh, Uttar Pradesh, IN
Source
Indian Journal of Health and Wellbeing, Vol 6, No 7 (2015), Pagination: 700-705Abstract
Breast cancer is the most prevalent type of cancer in female worldwide, with almost 1.7 million new cases diagnosed in 2012. This represents about 12% of all new cancer cases and 25% of all cancers in female. Emotional well being is just as essential as the wellness of cancer patients. Health and wellness are an unique state of mind that begins with a conscious decision to shape a healthy lifestyle and adopt it as a priority. It is important to recognize that health and wellness are crucial in personal satisfaction and optimizing quality of life. The diagnosis of a serious illness, such as cancer, may be a time when emotional concerns may need to be addressed. The purpose of this study was to examine the relationship among mindfulness-based intervention, emotional wellbeing, wellness and quality of life in patients of breast cancer. Mindfulness is the intentional, accepting and non-judgmental focus of one's attention on the emotions, thoughts and sensations occurring in the present moment. Review of the research literature indicated that mindfulness-based intervention was a significant predictor of quality of life, emotional wellbeing and wellness. The present study collated results from various studies, to demonstrate the potential efficacy of mindfulness-based interventions as it could be applied to patients with breast cancer to improve emotional wellbeing, wellness and quality of life. Clinical implications exist for counselors, psychologist and health care. Further research investigating possible moderating acceptance of mindfulness-based intervention among patients of breast cancer is needed.Keywords
Mindfulness-Based Intervention, Emotional Well-Being, Wellness, Quality of Life, Breast Cancer.
- Isolation and Identification of Mannheimia haemolytica by Culture and Polymerase Chain Reaction from Sheep’s Pulmonary Samples in Shiraz, Iran
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Materials and Methods: In this study, a total of 2500 sheep’s lungs were evaluated for finding pneumonic effects. Then, 161 infected pneumonic samples of lung tissues were investigated by culture and PCR methods.
Results: After cultivation, purification, and DNA extraction, 38 samples were found positive for M. haemolytica by cultivation and then all the 38 isolates were confirmed by PCR and multiplex PCR (mPCR). Results of this study indicated that culture and PCR are both practical in identification and isolation of this bacterium though culture is more time-consuming. The utilized mPCR has been more successful in the identification of the bacteria since it requires less time and cost.
Conclusion: In this study, PCR as a superior method among other methods of bacteriology for fast examination of infectious diseases and mPCR, which is a valuable tool for identification of M. haemolytica in clinical samples of animals, was used.
Authors
Affiliations
1 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IR
1 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IR
Source
Veterinary World, Vol 11, No 5 (2018), Pagination: 636-641Abstract
Background and Aim: Mannheimia haemolytica is a Gram-negative, non-motile, and non-spore-forming rod-shaped coccobacillus bacterium. On blood agar plate, it shows complete hemolysis. This bacterium constitutes a part of normal flora of the upper respiratory system of ruminants. It is considered as the opportunistic pathogen and the main factor of pneumonic pasteurellosis, which has caused a severe economic loss in sheep and cattle industries. Considering the prevalence of the disease in sheep and goat population in the dry and hot regions of the country in general and in Fars province in particular in the form of pneumonia, the purpose of this study was to isolate and identify the bacterium M. haemolytica from the lung tissues of sheep slaughtered in Shiraz abattoir through culturing and polymerase chain reaction (PCR) methods.Materials and Methods: In this study, a total of 2500 sheep’s lungs were evaluated for finding pneumonic effects. Then, 161 infected pneumonic samples of lung tissues were investigated by culture and PCR methods.
Results: After cultivation, purification, and DNA extraction, 38 samples were found positive for M. haemolytica by cultivation and then all the 38 isolates were confirmed by PCR and multiplex PCR (mPCR). Results of this study indicated that culture and PCR are both practical in identification and isolation of this bacterium though culture is more time-consuming. The utilized mPCR has been more successful in the identification of the bacteria since it requires less time and cost.
Conclusion: In this study, PCR as a superior method among other methods of bacteriology for fast examination of infectious diseases and mPCR, which is a valuable tool for identification of M. haemolytica in clinical samples of animals, was used.
Keywords
Mannheimia haemolytica, Pneumonia, Polymerase Chain Reaction, Sheep, Shiraz.Full Text
References
- Bojesen, A.M., Larsen, J., Pedersen, A.G., Morner, T., Mattson, R., and Bisgaard, M. (2007) Identification of a novel Mannheimia granulomatis lineage from the lesions in roe deer (Capreolus capreolus). J. Wildlife. Dis., 43: 345-352.
- Angen, O., Mutters, R., Caugant, D.A., Olsen, J.E. and Bisgaard, M. (1999) Taxonomic relationships of the Pasteurella haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb, nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. Int. J. Syst. Evol. Microbiol., 49: 67-86.
- Zecchinon, L., Fett, T. and Desmecht, D. (2005) How Mannheimia haemolytica defeats host defense through a kiss of death mechanism. Vet. Res., 36: 133-156.
- Quinn, P.J., Markey, B.K., Leonard, F.C., Hartigan, P., Fanning, S. and Patrick, E.S.F. (2011). Veterinary Microbiology and Microbial Disease. John Willy & Son Ltd, UK.
- Alexander, T.W., Shaun, R.C., Yanke, L.J., Booker, C.W., Morley, P.S., Read, R.R., Gowe, S.H.P. and McAllister, T.A. (2008) A multiplex polymerase chain reaction assay for the identification of Mannheimia haemolytica, Mannheimia glucosida and Mannheimia ruminalis. Vet. Microbiol., 130: 165-175.
- Ozbey, G. and Muz, A. (2006) Isolation of aerobic bacteria from the lungs of chickens showing respiratory disorders and confirmation of Pasteurella multocida by polymerase chain reaction (PCR). Vet. Archiv., 76: 217-225.
- Weiser, G.C., DeLong, W.J. and Paz, J.L. (2003) Characterization of Pasteurella multocida associated with pneumonia in bighorn sheep. J. Wildlife. Dis., 39: 536-544.
- Engdaw, T.A. and Alemneh, A.T. (2015) Pasteurellosis in small ruminants: Biochemical isolation, characterization and prevalence determination in relation to associated risk factors in Fogera Woreda, North-West Ethiopia. Adv. Biol. Res., 9: 330-337.
- Marru, H.D., Anijajo, T.T. and Hassen, A.A. (2013) A study on ovine pneumonic pasteurellosis: Isolation and identification of Pasteurella and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia. BMC. Vet. Res., 9: 239-244.
- Abera, D., Sisay, T. and Birhanu, T. (2014) Isolation and identification of Mannheimia and Pasteurella species from pneumonic and apparently healthy cattle and their antibiogram susceptibility pattern in Bedelle District, Western Ethiopia. Afr. J. Bacteriol. Res., 6: 32-41.
- Kaoud, H., El-Dahshan, A.R., Zaki, M.M. and Nasr, S.A. (2010) Occurrence of Mannheimia haemolytica and Pasteurella trehalosi among ruminants in Egypt. New York. Sci. J., 3: 135-141.
- Deressa, A., Asfaw, Y., Lubke, B., Kyule, M.W., Tefera, G. and Zessin, K.H. (2010) Molecular detection of Pasteurella multocida and Mannheimia haemolytica in sheep respiratory infections in Ethiopia. Int. J. Appl. Res. Vet. M., 8: 101-108.
- Kumar, J., Dixit, S.K. and Kumar, R. (2015) Rapid detection of Mannheimia haemolytica in lung tissues of sheep and from bacterial culture. Vet. World, 8: 1073-1077.
- Sabiel, Y.A., Musa, M.T. and Ann, V.Z. (2012) Identification of Mannheimia haemolytica and Pasteurella multocida by polymerase chain reaction and random amplification of polymorphic DNA. Sudan J. Vet. Res., 27: 55-59.