Informatics Publishing Limited

Dinesh Rao ¹, P. R. Pathak , Dongre , Divya Sood

Affiliations
1 Dept. of Forensic Medicine, Sri Siddhartha Medical College, Tumkur, Karnataka, IN

Abstract

The present study was on Incidence of Disseminated Intravascular Coagulation(DIC) in routine autopsies. In this Four year period (Jan 2003 to Dec 2006) of study, a total of 418 Medico legal autopsies were conducted of which 21 cases proved DIC. The study has been ventured primarily based on morbid anatomical and histo-pathological findings. Wherever possible clinical orientation has been attempted perusing antecedent case history and case records as and when available. The presence of single Microthrombi in minimum of one of the organs was considered to be DIC. The study also pointed out the discrepancies between clinical diagnosis and autopsy findings and also pointed out the clinical lapses in its diagnosis. The study also highlighted the incidental findings of DIC in Burns and Trauma cases, & also highlited the need for the alertness of Clinicians in cases suspected to manifest into DIC. Of the total number of DIC(n=21) cases only 12 were treated in the Hospital out of which only 2 cases were suspected for DIC. The majority of Microthrombi was in Kidney(71.43%) and Lungs(66.66%). and the least was spleen(n=1). The maximum haemorrhage were reported in Kidney(n=11) and the least was in the Liver and Brain. The external manifestation of Haemorrhage was only in few (n=3) cases. The study also highlighted the importance of DIC as Immediate Cause of death due to various underlying mechanisms.

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Amit Anandswarup Vanka ¹, Shanthi Vanka ², Sandeep Vishwakarma ³, Manohar Bhat ⁴, Dinesh Rao ⁵, Othman Wali ⁶, Aleem Khan ³

Affiliations
1 Pacific Academy of Higher Education and Research University, Udaipur − 313003, Rajasthan, IN
2 Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah − 22421, SA
3 Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, IN
4 Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur − 302015, Rajasthan, IN
5 Department of Pedodontics and Preventive Dentistry, Pacific Dental College and Hospital, Udaipur − 313011, Rajasthan, IN
6 Ibn Sina National College for Medical Studies, Jeddah − 22421, SA

Abstract

Platelet Rich Plasma (PRP) has the potential to regenerate pulp in immature pulpless teeth. Mineral trioxide is commonly used to seal the PRP into the pulp canal space. We investigated the effect of PRP and MTA individually and combined on osteo/odontogenic differentiation potential and the phenotype of tissue formed. MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5% PRP and MTA with 10% PRP. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Relative expression of Alkaline Phosphatase (ALP) activity, type 1 collagen (COL1A1), Dental Sialo-Phospho Protein (DSPP), Dentin Matrix Protein (DMP-1), Bone Gamma-carboxyglutamate Protein (BGLAP), Runt-related transcription factor 2 (Runx2), Osterix (Osx) and TGF-β1 was identified by RT-q PCR. 10% PRP with MTA displayed significantly higher calcium deposition during differentiation and high ALP levels. Significantly enhanced levels of DSPP, DMP1, COL1A1, BGLAP, Runx2, and Osx and TGF-β1 transcripts were observed. Within limitations of the in vitro environment, results imply enhanced osteodentin formation, on combining PRP with MTA.

Keywords

Platelet Rich Plasma, Mineral Trioxide Aggregate, Mesenchymal Stem Cells, Odontogenic, Differentiation.

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Pratiksha Marathe ¹, Simran H. Dusseja ¹, Dinesh Rao ², Sunil Panwar3 Panwar ³, Safna Ameen ⁴

Affiliations
1 Postgraduate Student, Department of Pediatric Dentistry, Pacific Dental College and Hospital, Udaipur – 313024, Rajasthan, IN
2 Professor and Head, Department of Pediatric Dentistry, Pacific Dental College and Hospital, Udaipur – 313024, Rajasthan, IN
3 Professor, Department of Pediatric Dentistry, Pacific Dental College and Hospital, Udaipur – 313024, IN
4 Senior Lecturer, Department of Pediatric Dentistry, Pacific Dental College and Hospital, Udaipur – 313024, Rajasthan, IN

Abstract

Background: Focal Reactive Gingival Overgrowths (FRGO) manifest as a result of chronic stimuli in the form of fibrous connective tissue lesions in the oral mucosa which are routinely observed in day-to-day dental practice. Gingiva is the frequently affected site for such lesions. Along with the clinical presentation, the histopathological investigation of the lesion plays a crucial role for the confirmation of the diagnosis. This case report deals with the surgical management of pyogenic granuloma and irritational fibroma. The first case is treated by using a modified surgical excision in an attempt to prevent the lesion from recurring. On following of both the cases after one month it was observed that the lesions did not recur. Elimination of the cause along with parental counselling are the essential factors for the successful outcome in such cases.

Keywords

Irritational Fibroma, Modified Surgical Excision, Pyogenic Granuloma, FRGO

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