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S. Sundravadana ¹, D. Alice ¹

Affiliations
1 Department of Plant Pathology Centre for Plant Protection Studies (TN AU) Coimbatore 641 003, Tamil Nadu, IN

Abstract

Antifungal activity of zinc sulphate against Macrophomina phaseolina, causing ischolar_main rot disease in black gram was investigated under in vitro condition. The result of experiment revealed that among the nine nutrients tested, zinc sulphate at 500 and 750 ppm completely inhibited the mycelial growth of M. phaseolina. Synergistic action of zinc sulphate with T. viride showed increased mycelial growth (66.25mm, 207x10⁶ spore/sq. cm), mycelial dry weight (565.22 mg) and sporulation (81.80 x 10⁶ spores/ml) produced at 500 ppm and hence T. viride significantly reduced the mycelial growth of M. phaseolina in zinc sulphate amended medium.

Keywords

Macrophomina phascolina, Nutrients, Trichoderma virdie, Synergistic Action, Zinc Sulphate.

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M. P. Uma Maheswari ¹, M. Muthusamy ¹, D. Alice ²

Affiliations
1 Agricultural College and Research Institute, Killikulam, Vallanad 628 252, Tamil Nadu, IN
2 Agricultural College and Research Institute, Coimbatore 641 003, Tamil Nadu, IN

Abstract

The effectiveness of four species of Trichoderma namely T. viride Pres. ex. S. F. Gray, T. longibrachiatum Rifai, T. reesei Simmons, and T. harzianum Rifai, Gliocladium virens J. H. Miller, Giddens and Foster, two bacterial antagonists namely Pseudomonas fluorescens Migula and Bacillus subtilis (Chun) Praznowski and Saccharomyces cerevisiae Hansen was tested against Sclerotium rolfsii Saec. causing jasmine (Jasminum sambac L.) wilt. P. fluorescens, B. subtilis and T. viride were found effective against sclerotial wilt. The volatile metabolites produced by these antagonists were also effective against S. rolfsii in vitro. The sclerotial germination of S. rolfsii was effectively inhibited by P. fluorescens, B. subtilis and T. viride. All the antagonists tested, except S. cerevisiae were effective in inhibiting the production of oxalic acid. Soil application of talc based commercial formulation of P. fluorescens @ 20 g/pot, B. subtilis and T. viride @ 25g/pot effectively reduced the wilt disease incidence in the pot culture experiment.

Keywords

Bacillus subtilis, Jasmine Wilt, Oxalic Acid, Pseudomonas fluorescens, Sclerotium rolfsii, Talc Based Commertial Formulations, Trichoderma viride, Volatile Metabolites.

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