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Ramasubramanian, Abilasha
- Liquid Paraffin as a Rehydrant for Air Dried Buccal Smear
Authors
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1197-1200Abstract
Aim: To assess the efficacy of liquid paraffin as a rehydrant for air dried buccal smear based on the nuclear and cytoplasmic details. Background: Buccal smear is useful for diagnosing Malignancy, Fungal infection, Viral infection and Vesicullobullous dermatoses. The routine practice is to wetfixthe smear and send them to the laboratory for staining and evaluation by a cytopathologist. But drying of smears is inevitable, especially when the aspirate is less and when there is improper fixation. This can cause drying artifacts. An alternative method for overcoming this problem is intentional air drying followed by rehydration. Many rehydrants have been experimented upon. The most common rehydrant being saline. The present study is done to check the efficacy of liquid paraffin as a rehydrant. Material and methods: 2 sets of 20 buccal smears were collected from the patients. One set air dried for 24 hours and the other wet fixed. Conventional pap staining was then carried out. The slides were examined by two observers for preservation of Nuclear and cytoplasmic details based on the semi quantitative scoring system. Results: Excellent nuclear details were seen in 45% of air dried smear compared to 25%in routine wet fixation. The cytoplasmic details of air dried smear and routine wet fixed smear was the same. Conclusion: Liquid paraffin as a rehydrant has shown promising results and the nuclear details were found to marginally better than in routine wet fixed smear. Air dried buccal smears can be used in routine practice especially in rural areas where there is limited access to laboratories and for mass screening.Keywords
Buccal smear, Rehydration, Liquid Paraffin.
References
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- General Awareness of Diabetes Mellitus among a Hospital Population in Chennai:A Survey
Authors
1 Department of Oral Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, IN
Source
Indian Journal of Public Health Research & Development, Vol 11, No 1 (2020), Pagination: 12-15Abstract
Aim and Objective: The aim of this study is to assess the awareness of diabetes mellitus and knowledge about the various aspects of diabetes including types, treatment modalities, preventive measures and lifestyle modification in the patient population visiting a Dental Hospital in Chennai.
Materials and Method: In this study, a questionnaire was prepared and circulated among the subjects to determine the awareness and to inspect the knowledge about the symptoms, diagnosis and preventive measures about Diabetes mellitus among the people. The study sample comprised of totally 100 subjects, both males and females of age group between 20 and 50 years.
Results: According to the survey 52% of the individuals are aware about that diabetes can lead to further complications. 82% of the individuals felt that the diabetes can be controlled by exercise. 19% of individuals have not undergone any confirmatory tests for diabetes. 50% of the individuals were under medication for treatment of diabetes and they experienced side effects while taking those medications. 50% of the individuals who participated in the survey have attended a formal diabetes educational programme and they are aware of diabetes through media,relatives and doctors.