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Lakshmana Rao, A.
- Hepatoprotective Activity of Alstonia scholaris
Abstract Views :380 |
PDF Views:182
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Asian Journal of Pharmaceutical Research and Health Care, Vol 4, No 1 (2012), Pagination: 28-32Abstract
The hepatoprotective activity of ethanolic extract of fruits of Alstonia scholaris was evaluated by carbon tetrachloride (CCL4) - induced hepatoto xicity in rats. The toxicant CCL4 was used to induce hepatotoxicity at a dose of 2 ml/kg as 1:1 mixture with olive oil. The ethanolic extract of fruits of Alstonia scholaris was administered in the dose of 150 and 300 mg/kg/day orally for 5 days. Silymarin (50 mg/kg) was used as standard drug. The hepatoprotective effect of th e ethanolic extract was evaluated by the assessment of biochemical parameters such as SGOT, SGPT, SALKP , total bilirubin and histopathological studies of the liver. Treatment of animals with the ethanolic extract significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver as indicated by lower levels of serum bilirubin as compared with the normal and silymari n treated groups.Keywords
Carbon Tetrachloride, Hepatoprotective Activity, Alstonia scholaris, Silymarin.- Estimation of Gatifloxacin in Pharmaceutical Dosage Forms by High Performance Liquid Chromatography
Abstract Views :305 |
PDF Views:173
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Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 3, No 3 (2011), Pagination: 72-76Abstract
A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of gatifloxacin in its pharmaceutical dosage forms. In this method RP - C 18 column (150mmx4.6mm I.D., 5 μm particle size) with mobile phase consisting of acetonitrile and 0.05M phosphate buffer in the ratio of 25:75 v/v in isocratic mode was used. The detection wavelength is 293nm and the flow rate is 0.8ml/min. Ciprofloxacin is used as internal standard. The linearity was fo und to be in the range of 0.1 to 10 μg/ml and shows a correlation coefficient of 0.9999. The intra - and inter - day variation was found to be less than 1.2% showing high precision of the assay method. The mean percent recovery of the drug from the solution c ontaining 4 μg/ml was 100.5 ± 2.06 indicating high accuracy of the proposed method. Hence, this method is simple, fast, specific, accurate, precise and less time consuming for the estimation of gatifloxacin in pharmaceutical dosage forms.Keywords
HPLC, Gatifloxacin, Estimation, Pharmaceutical Dosage Forms.- A New Validated RP-HPLC Method for the Estimation of Mycophenolate Mofetil in Pure and Tablet Dosage Form
Abstract Views :460 |
PDF Views:203
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Asian Journal of Pharmaceutical Research and Health Care, Vol 2, No 3 (2010), Pagination: 266-269Abstract
A simple, rapid, sensitive and precise high performance liquid chromatographic (HPLC) method has been developed for the estimation of mycophenolate mofetil in bulk and tablet dosage form. In this method RP-C18 column (250mmx4.6mm I.D., 5im particle size) with mobile phase consisting of acetonitrile and 0.03M phosphate buffer in the ratio of 60:40 v/v in isocratic mode was used. The detection wavelength is 254 nm and the flow rate is 0.8 ml/min. In the range of 20-120μg/ml, the linearity of mycophenolate mofetil shows a correlation coefficient of 0.9995. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters. The mobile phase selected for the method is simple, fast, accurate and precise and hence can be applied for routine quality control analysis of mycophenolate mofetil in bulk and its tablet dosage form.Keywords
HPLC, Mycophenolate Mofetil, Validation.- Caco-2 Cells: An Overview
Abstract Views :327 |
PDF Views:173
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Asian Journal of Pharmaceutical Research and Health Care, Vol 1, No 2 (2009), Pagination: 260-275Abstract
The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells. Human colonic adenocarcinoma cells that are able to express differentiation features characteristic of mature i ntestinal cells, such as enterocytes or mucus cells. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug tra nsport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.Keywords
Caco-2 Cells, Passive Transport, Apoptosis.- A Simple and Sensitive RP-HPLC Method for Estimation of Trandolapril in Bulk and Tablet Dosage Forms
Abstract Views :253 |
PDF Views:0
Authors
Affiliations
1 Sri Vasavi Institute of Pharmaceutical Sciences, Tadepalligudem-534 101, A.P., IN
2 V.V. Institute of Pharmaceutical Sciences, Gudlavalleru-521 356, A.P., IN
1 Sri Vasavi Institute of Pharmaceutical Sciences, Tadepalligudem-534 101, A.P., IN
2 V.V. Institute of Pharmaceutical Sciences, Gudlavalleru-521 356, A.P., IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1425-1427Abstract
An accurate and precise HPLC method was developed for the determination of trandolapril. Separation of the drug was achieved on a reverse phase C18 column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 35:65 v/v. The flow rate was 0.8 ml/min and the detection wavelength was 210 nm. The linearity was observed in the range of 20-60 μg/ml with a correlation coefficient of 0.999. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of trandolapril in tablet dosage forms.Keywords
Trandolapril, Estimation, RP-HPLC, Validation, Tablets.- Development and Validation of New HPLC Method for Estimation of Lornoxicam in Bulk and Tablet Dosage Forms
Abstract Views :232 |
PDF Views:0
Authors
B. Raja
1,
A. Lakshmana Rao
2
Affiliations
1 Anurag Pharmacy College, Kodad-508206, A.P., IN
2 V.V. Institute of Pharmaceutical Sciences, Gudlavalleru- 521356, A.P., IN
1 Anurag Pharmacy College, Kodad-508206, A.P., IN
2 V.V. Institute of Pharmaceutical Sciences, Gudlavalleru- 521356, A.P., IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1081-1083Abstract
An accurate and precise RP-HPLC method was developed for the determination of lornoxicam in bulk and tablet dosage forms. Separation of the drug was achieved on a reverse phase C18 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 65:35 v/v. The pH was adjusted to 3.0 with orthophosphoric acid. The flow rate was 1.5 ml/min and the detection wavelength was 289 nm. The linearity was observed in the range of 30-150 μg/ml with a correlation coefficient of 0.999. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lornoxicam in tablet dosage forms.