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V. S. Nade , L. A. Kawale , S. Dwivedi , A. V. Yadav

Abstract

Objective: To investigate the effect of the methanolic extract of Hibiscus rosa sinensis ischolar_mains (H. sinensis) on the central nervous system in mice and rats. Materials and methods: The methanolic extract of H. sinensis was studied on pentobarbital-induced sleeping time, elevated plus maze, hole board test, foot shock-induced aggression, haloperidol-induced catalepsy, lithium-induced head twitches, locomotor activity, acetic acid-induced writhing and hot plate analgesiometer at various doses (50 - 200 mg/kg), intraperitoneally for sedative, anxiolytic, antipsychotic, muscle relaxant and analgesic activities. Results: The extract prolonged pentobarbital-induced sleeping time indicating sedative effect. The extract increased the time spent in open arms and the number of head dips in elevated plus maze and hole board test respectively demonstrating anxiolytic activity. H. sinensis decreased number of fights in foot shock-induced aggression and potentiated haloperidol-induced catalepsy in dose dependent manner showing antipsychotic effect. The extract significantly inhibited head twitches induced by lithium suggesting its inhibitory effect on serotonergic system. The H. sinensis significantly decreased the locomotor activity in dose dependent manner. The extract showed potential analgesic activity in acetic acid-induced writhing and hot plate analgesiometer. The H. sinensis attenuated the behavior mediated by the dopaminergic and serotonergic system and potentiated the GABAergic actions. Conclusion: The results suggest that the methanolic extract of Hibiscus rosa sinensis has potential neuropharmacological activities.

Keywords

Hibiscus Rosa Sinensis, Sedative, Anxiolytic, Antipsychotic, Analgesic, Catalepsy

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V. S. Nade , A. V. Yadav

Abstract

The present study was designed to investigate the neuroprotective effect of Hibiscus rosa sinensis L. in aluminuminduced neurotoxicity in rats. The animals were treated with aluminium chloride (4.2 mg/kg, p.o) for 21 days to induce oxidative stress. The methanolic extract of Hibiscus rosa sinensis (HRS - 100, 200 and 300 mg/kg/day, p.o, for 21 days) was administered 30 min before aluminum. The elevated plus-maze paradigm was used to assess the effect of HRS on aluminum-induced memory impairment. Animals were sacrificed on 21st day, brains were isolated and biochemical estimations were performed. The aluminium treated group showed significant increase in transfer latency (TL) on day 20th and 21st indicating impairment of memory. Administration of HRS (100 - 300 mg /kg, p.o) significantly (p < 0.01) reversed the memory impairment. Biochemical analysis of brain revealed that the chronic administration of aluminium significantly increased lipid peroxidation and decreased levels of catalase (CAT) and glutathione reductase (GSH), an index of oxidative stress process. Administration of extract significantly (p < 0.01) attenuated the lipid peroxidation; and reversed the decrease in brain CAT and GSH levels (p < 0.01). The superoxide dismutase (SOD) levels were increased after aluminium treatment which was normalized by HRS (p < 0.01). The results strengthen the oxidative stress hypothesis of aluminium- induced neurotoxicity and suggest the beneficial role of HRS in the management of Alzheimer's disease and oxidative stress. Cognitive-enhancing activity of HRS may be exerted through antioxidant mechanism.

Keywords

Hibiscus rosa sinensis, Aluminium, Elevated Plus-maze, Lipid Peroxidation, Oxidative Stress

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H. J Pramod ¹, A. V. Yadav ¹, V. N. Raje ¹, Madhuri Mohite ¹, Ganesh Wadkar ¹

Affiliations
1 K.L.E. University, Department of Pharmacognosy and Phytochemistry, J.N.M.C Campus, Nehru Nagar, Belgaum, Karnataka-590010, IN

Abstract

The fruit of Borassus flabellifer Linn. belonging to family Areaceae reported to be posses the antioxidant activity. The extracts have been also evaluated for antioxidant activity by using in-vitro methods DPPH (2, 2-Diphenyl-1 picryl hydrazyl) and ABTS (2, 2-azinobis-(3-ethylbenzo-thiazoline-6-sulfonic acid) assay. The phytochemical analysis carried out revealed the presence of saponins, tannins, flavonoids, carbohydrates, amino acids and phenolic compounds in the extracts.

Keywords

Antioxidant, DPPH, ABTS, Borassus flabellifer Linn

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M. S. Mohite ¹, A. V. Yadav ², V. N. Raje ², Y. G. Mohite ², S. A. Thombre ²

Affiliations
1 Department of Pharmacognosy, Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara, IN
2 Department of Pharmacognosy, Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara, Maharashtra, IN

Abstract

Borassus flabellifer Linn. (Areaceae) is a tall tree (palm) growing in sandy soil and attaining a height of 20-30 meters with straight trunk. The extraction was carried out around 300 gm of powder was subjected to successive hot continuous extraction (Soxhelt) with petroleum ether, ethyl acetate, chloroform, methanol, chloroform-water. The Phytochemical analysis carried out revealed the presence of Saponins, tannins, flavonoids, carbohydrates, amino acids and phenolic compounds in the extracts. For assessing in-vitro antioxidant activity total antioxidant capacity and determination of total phenolic content methods were used. In total antioxidant capacity aqueous extract was found a significant antioxidant activity. In determination of total phenolic content alcoholic extract shown highly significant result.

Keywords

Borassus flabellifer, Antioxidant, Pharmacognostic Investigation, Phytochemical Constituents.

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M. S. Mohite ¹, Poonam Shelar ¹, Pravin Pawar ¹, A. V. Yadav ¹, Rutuja Gadhave ¹, Omkar Bhandwalkar ¹

Affiliations
1 Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara, IN

Abstract

Stain removal is the process of removing a mark or spot left by one substance on a specific surface like fabric. The objective of present investigation is to formulate herbal stain removal for removing the stain from the fabrics. A solvent or detergent is generally used to conduct stain removal & many of these are available over another. In the present study stain removal was formulated by using herbal ingredients like Eucalyptus oil, Tween 80, Poloxomer, Citric acid, Carboxy methyl cellulose, Rose oil, Neem oil. The three different formulae were formulated viz. formula F1, formula F2 and formula F3. The six different samples of stain were used for observation of stain removal like tea, ketchup, ink, iodine, mud, blood. The action of prepared formulation was seen on these stained fabrics. All the formulations shows positive results and formula F3 was shows the best results than other formulations.

Keywords

Stain Removal, Eucalyptus Oil, Tween 80, Poloxomer.

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V. Y. Lokhande ¹, A. V. Yadav ²

Affiliations
1 Government College of Pharmacy ,Karad, MS, IN
2 Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara, MS, IN

Abstract

Human beings have been using the plants for treating their injuries, disorders since ancient times. With modernization of medical science these uses have been exploited for inventions of many drugs. But still many tribes are using drugs in their primitive form and knowledge of such formulations is limited to their community. This traditional knowledge is hardly communicated to people other than the members of community. This knowledge must be recorded, documented and an attempt to establish scientific correlation should be made. Kaas plateu and the region is a biodiversity hotspot and local people are still using the natural remedies for treating the disorders. The current ethnomedicinal survey is aimed to identify, collect such plants used by local people to treat ailments like convulsions, malaria and jaundice. Information of such plants and way of using them was collected. 10 plants were identified and their information was recorded. The information pooled from this survey can be further used for establishing the scientific data pertaining to these claims. If such claims are proven scientifically standardized methods can be developed for the reliable and reproducible use of these remedies.

Keywords

Ethnomedicinal Survey, Kaas Plateu, Traditional Remedies, Malaria, Jaundice, Convulsions.

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