Indian Journal of Public Health Research & Development

Desty Renata ¹, Didik Handijatno ¹, Wiwiek Tyasningsih ¹

Affiliations
1 Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID

Abstract

Septicemia Epizootica (SE), is a fatal acute septicemia disease by Pasteurella multocida. B:2,5 and E:2,5 in cattle and buffalo. TbpA has a number of important role for bacterial cells, such as nutrient uptake, transport of molecules in and out of cells and interactions with the environment and host. P. multocida iron acquisition related gene, tbpA, has been considered as an important epidemiological marker. The purpose of this study was to compare the Transferrin Binding Protein A (TbpA) gene from P. multocidatype B of buffalo and it with vaccine strain. Characterization of TbpA of P. multocida and vaccine strain was done using PCR then sequencing. Sample used in this study were P. multocida local isolate from NTT and vaccine strain (Katha). Isolation and identification were performed using bacteriological culture and biochemically characterization. Results from PCR showed positive a tbpA gene on P. multocida local isolate and vaccine strainis. The comparison of the results of sequencing between the tbpa gene isolates P.multocida and vaccines showed deletion.

Keywords

Septisemiaepizootica, Pasteurellamultocida Type B, Transferrin Binding Protein A (TbpA) Gene, PCR, Buffalo.

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Firdausy Kurnia Maulana ¹, Didik Handijatno ², Hani Plumeriastuti ³, Rahaju Ernawati ², Wiwiek Tyasningsih ², Mufasirin ⁴

Affiliations
1 Airlangga University, ID
2 Department of Microbiology and Mycology, Airlangga University, ID
3 Department of Veterinary Pathology, Airlangga University, ID
4 Department of Veterinary Parasitology, Airlangga University, ID

Abstract

Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Outer membrane Protein H (OmpH) is one of the major proteins in P. multocida envelope has been purified and characterized as porin immunodominant. The aim of this study is to find out the homology of nucleotide sequence of 37 kDa OmpH gene of P. multocida of local isolate from NTT with vaccine strain (Katha) and 5 referen isolates and predict the epitope of 37 kDa OmpH gene. Detection of 37 kDa OmpH gene was done using PCR. Homology analysis of nucleotide and amino acid sequences was performed on the BLAST program at NCBI. Epitope prediction of 37 kDa OmpH genes P. multocida from NTT and vaccine strains was performed using the online programme B Cell Epitope Prediction Tools. The results showed that 37 kDa OmpH gene of P. multocida from NTT has 99% homology to vaccine strain (Katha) and isolates from China, Iran, India, Mesir, and 89% homology to isolate from USA and 37 kDa OmpH gene of P. multocida local isolate from NTT were likely to be immunogen candidates based on B cell epitope that had 11 epitopes. These findings indicate that P. multocida local isolate from NTT has a chance as immunogen candidate, which can be developed as a vaccine candidate and diagnostic kit for HS.

Keywords

Haemorrhagic Septicaemia, P. Multocidatype B, Outer Membrane Protein H(omp H) Gene, Pcr, Sequence, Epitope.

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Naimah Putri ¹, Rahaju Ernawati ², Suwarno ², Kusnoto ³, Wiwiek Tyasningsih ⁴, Fedik Abdul Rantam ⁵

Affiliations
1 Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
2 Laboratory of Virology and Immunology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
3 Laboratory of Parasitology Department Parasitology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
4 Laboratory of Bacteriology and Micology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
5 Stem Cell Research and Development Center, Universitas Airlangga, ID

Abstract

This study were conducted to analyze the homology and the relation of nucleotidesencoding partial genomic sequence of Fusion (F) protein Newcastle Disease Virus (NDV) isolated from swanusing phylogenetic tree. Samples were collected from cloacal swabs of seventy one waterfowls. Consisting of twenty four Domestic swan, thirty three Muscovy ducks, and fourteen Domestic ducks form waterfowl in East Java Province, and also positive LaSota isolate (ATCC). Samples were isolated in embryonated chicken eggs(ECEs) with specific pathogen free (SPF)and identified by HA test and confirmed as NDV by HI test. Positive samples were performed by RT-PCR using a forward and reverse primer with a target of 699 bp.The nucleotide sequences from field isolates were compared with the gene of vaccine viruses isolates encoded F protein NDV that used in Indonesia and vaccine viruses encoded F protein NDV that spread across the globe obtained at GenBank data and had very close phylogenetic relation with those vaccine viruses LaSota.Analysis of homology and phylogenetic from isolates in this study relate to isolate of vaccine viruses.

Keywords

Newcastle Disease Virus, F Protein, Homology, Phylogenetic Tree, RT-PCR.

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