Indian Journal of Public Health Research & Development

Nur Syuhada Mohd Ataa ¹, Siti Salwa Abd Gani ², Mohd Izuan Effendi Halmi ³, Sumaiyah Abdullah ⁴, Masriana Hassan ⁵, Uswatun Hasanah Zaidan ⁶, Valerie Orsat ⁷

Affiliations
1 Universiti Putra Malaysia, Serdang, Selangor, MY
2 Department of Agriculture Technology, Universiti Putra Malaysia, Serdang, Selangor, MY
3 Department of Land Management, Universiti Putra Malaysia, Serdang, Selangor, MY
4 Department of Plant Protection, Universiti Putra Malaysia, Serdang, Selangor, MY
5 Department of Pathology, Universiti Putra Malaysia, Serdang, Selangor, MY
6 Department of Biochemistry, Universiti Putra Malaysia, Serdang, Selangor, MY
7 Department of Bioresource Engineering, Macdonald Stewart Building, Ste-Anne-de-Bellevue, QC, CA

Abstract

Two plant species from the Melastomataceae family, Melastomamalabathricum Linn. and Melastomadecemfidum Roxb, which grow in abundance in tropical and subtropical regions have been well-documented to possess various medicinal values including anti-inflammatory properties. The present study was undertaken to examine the anti-inflammatory potential of M. Malabathricum and M. Decemfidum methanolic extracts (MME and MDE respectively) on lipopolysaccharide (LPS)-induced macrophage cell line (RAW 264.7). The cytotoxic assays of MME and MDE against RAW264.7 cells were conducted using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Production of nitric oxide (NO) from both Melastoma sp. was measured using Griess assay. MME and MDE exhibited high cell viability (>90%) at concentrations from 0.39 to 1.56μg/mL and 6.25 μg/mL respectively. Although the results obtained were not significantly different from control,NO production by LPS-induced RAW264.7 cells were observed to be lower for both extracts at concentration of1.5μg/mL when compared to untreated cells (control). The present study confirmed that MME and MDE have the potential to inhibit anti-inflammatory mediator against LSP-induced RAW264.7 cells.

Keywords

Anti-inflammatory, Melastomamalabathricum, Melastomadecemfidum, RAW264.7 Cell line.

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