Indian Journal of Public Health Research & Development

Nunuk Helilusiatiningsih ¹, Yunianta ², Harijono ², Simon Bambang Wijanarko ²

Affiliations
1 Faculty of Agriculture, Universitas Islam Kadiri, ID
2 Faculty of Agriculture Technology, Universitas Brawijaya Malang, ID

Abstract

Solanum torvum has antioksidan. This study aimed to analyse cytotoxic activity and selectivity index of Solanum torvum on T47D breast cancer cells invitro. Identification of Solanum torvum fruit chemical compounds with LCMS containing Clorogenic acid, 4-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-feruloyquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,4-O-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, putative dicaffeoylquinic lactone. Cytotoxic assays showed that the Solanum torvum Without Fermentation were IC₅₀ (IGT) 1111.62 μg/ mL, IC₅₀ (MGT) 1889.20 μg/mL, IC₅₀ (EGT) 1171.90 μg/mL, IC₅₀ (FEGT) 305.98 μg/mL. Cytotoxic assays showed thatthe treatment of fermentation was IC₅₀ (IBT) 1760.36 μg/mL, IC₅₀ (MBT) 1631.06 μg/mL, IC₅₀ (EBT) of 1111.58 μg/mL, IC₅₀ (FEBT) 39.17μg/mL, IC₅₀ (FHBT) 85.58 μg/mL. Index of selectivity (IS) Solanum torvum (without fermentation) were IS (IGT) 5.23, IS (MGT) 0.61, IS (EGT) 1.06, IS (FEGT) 1.45. Index of selectivity of Solanum torvum (fermentation treatment) were IS (IBT) 2.41, IS (MBT) 0.78, IS (EBT) 0.18, IS (FEBT) 4.64, IS (FHBT) 1.67. The best results are treatment of ethyl acetate fraction of Solanum torvum(FEBT) has IC₅₀ was 39.17 μg/mL and Index selectivity was 4.64, declared safe/not toxic to normal cells, thus potentially developed as T47D breast anticancer cells.

Keywords

Cytotoxic, T47D Breast Cells, Selectivity Index, Solanum torvum.

Full Text

     

Nunuk Helilusiatiningsih ¹, Yunianta ², Harijono ², Simon Bambang Wijanarko ²

Affiliations
1 Faculty of Agriculture, Universitas Islam Kadiri, ID
2 Faculty of Agriculture Technology Universitas Brawijaya Malang, ID

Abstract

Solanum torvum has antioksidan. This study aimed to analyse cytotoxic activity and selectivity index of Solanum torvum on T47D breast cancer cells invitro. Identification of Solanum torvum fruit chemical compounds with LCMS containing Clorogenic acid, 4-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-feruloyquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,4 - O dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, putative dicaffeoylquinic lactone. Cytotoxic assays showed that the Solanum torvum Without Fermentation were IC₅₀ (IGT) 1111.62μg/ mL, IC₅₀ (MGT)1889.20μg/mL, IC₅₀ (EGT) 1171.90μg/mL, IC₅₀ (FEGT) 305.98 μg/mL. Cytotoxic assays showed that the treatment of fermentation was IC₅₀ (IBT) 1760.36 μg/mL, IC₅₀ (MBT) 1631.06 μg/mL, IC₅₀ (EBT) of 1111.58 μg/mL, IC₅₀ (FEBT) 39.17μg/mL, IC₅₀ (FHBT) 85.58 μg/mL. Index of selectivity (IS)Solanum torvum (without fermentation) were IS (IGT) 5.23, IS (MGT) 0.61, IS (EGT) 1.06, IS (FEGT) 1.45. Index of selectivity of Solanum torvum (fermentation treatment) were IS (IBT) 2.41, IS (MBT) 0.78, IS (EBT) 0.18, IS (FEBT) 4.64, IS (FHBT) 1.67. The best results are treatment of ethyl acetate fraction of Solanum torvum(FEBT) has IC₅₀ was 39.17 μg/mL and Index selectivity was 4.64, declared safe/not toxic to normal cells, thus potentially developed as T47D breast anticancer cells.

Keywords

Cytotoxic, T47D Breast Cells, Selectivity Index, Solanum torvum

Full Text