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Authors
Affiliations
1 Biotechnology of Fisheres and Marine, Pasca Sarjana, Airlangga University, ID
2 Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, ID
3 Department of Parasitology, Airlangga University,, ID
4 Department of Fish Health, Airlangga University, ID
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1714-1418
Abstract
Grouper cultivation is still constrained by the availability of seeds due to diseases caused by bacteria. Vibriosis is a disease caused by bacterial infections of the Vibrio genus such as Vibrio alginolyticus and Vibrio parahaemolyticus which are the most common in hatcheries. Whole Cell Vaccine (WCV) contains endotoxin components as antigens to stimulate antibody formation, including lipopolysaccharide from the cell wall of gram-negative bacteria. There is a problem of oral vaccination which can damagewhen it enters the low pH digestive system. Therefore, it is necessary to conduct a research to find out the ability of the Whole cell vaccine (WCV) of Vibrio alginoliticus bacteria by microencapsulation method of alginate gel as an oral alginate particle vaccine Vibrio alginoliticus to increase the survival of the grouper seeds of Epinephelus sp. This study used four types of treatment, namely control (-) without vaccine, control (+) using alginate only, dosage treatment 0.025 mg/5gr BB fish/day and 0.05 mg/5gr BB fish/day. The results of the Whole Cell Vaccine using alginate gel microcapsules process can increase the life range of cantang grouper seeds by 80% at 0.05 mg/5gr BB fish/day and 73% at 0.025 mg/5gr BB fish/day.
Keywords
Vibrio Alginolyticus, Whole Cell Vaccine, Alginate, Grouper, Vibriosis.
