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Authors
Affiliations
1 College of Agriculture, University of Anbar, IQ
2 College of Biotechnologies, University of Al Nahrain, IQ
Source
Indian Journal of Public Health Research & Development, Vol 10, No 11 (2019), Pagination: 2719-2724
Abstract
Total 150 birds of the Ross 308 type were raised in the fields of the Faculty of Agriculture/Anbar University. The breeding period lasted for 42 days. At the end of the breeding period, 60 birds were randomly selected. The blood samples of the birds were individually taken through the jugular vein. The genetic analysis was carried out in the laboratories of the Faculty of Biotechnology/University of Nahrain. The aim was to separate the genetic material and thus determine the genotype of the FGFBP1 gene. The study examined the diagnosis of genetic markers that are related to important characteristics of the carcass and the relationship between the study genes, the weights of the living birds, the measurements of the birds before the slaughter, and the characteristics of the carcass (carcass weight, net weight of the carcass and the percentage of carcasses, Abdominal fat in the carcass and internal weights of the intestines that include liver, heart and legumes) for the Ross 308 meat breeds are under study, thus identifying the frequency of the genotypes and replicating the gene to obtain the best possibility to improve the economic characteristics of the chicken. The FGFBP1 gene was identified in the current study birds based on the packages that resulted from the DNA transfer of the individuals after the electrical transfer was carried out using the PCR-RFLP technique and the restriction enzyme Pst 1. The gene fragments (AA, AB, BB) were identified for FGFBP1 gene and were replicates of the genotypes (30.00, 48.33, 21.67) respectively. The replication of gene A 0.54 and gene B 0.46 exceeded the genetic makeup of the gene FGFBP1 The rest of the genotypes in the vivo characteristics of the first week and the rate of increase in weight For weekly in the first week and the amount of feed consumed in the week, second, third and feed conversion efficiency in the first week, the fourth week and the length of the chest, chest width and depth of the chest in the first week of the period of education and carcass weight and net carcass and the weight of the liver, heart and gizzard exceed significantly (0.05>P). FGFBP1 mutations were identified after sequencing of mutations of 65% for each and 35% for the Ross 308 meat samples.
Keywords
FGFBP1, Sequencing, RFLP PCR. Broiler.
