Indian Journal of Public Health Research & Development

Athraa Y. Al-Hijazi ¹, Noor Natik Raheem ², Ali JB Al-Sharki ³

Affiliations
1 Professor at Al-Mustaqbal University College/Dentistry Department, Babel, IQ
2 Ass. Lecturer at College of Dentistry, Mustansiriya University, Baghdad, IQ
3 Lecturer at College of Dentistry,University of Baghdad, IQ

Abstract

Background: Bone is a high vascular tissue and resilient organ with intuitive healing ability. Bone tissue contains multipotent stromal cells which have the ability to differentiate into different specialized cells. Induced stem cells have been developed by various growth factors. Using of biomaterial and growth factor is one of the most commonly used as new tissue engineering strategies. Aim of the study: To evaluate the effect of application of a combination bone morphogenic protein 2 (BMP‑2) with brain-derived neurotrophic factor (BDNF) as biomaterial to enhance bone healing in osteoporotic rat . Materials and Method: Twenty female Wistar rat(10 normal rat and other 10 induced osteoporotic rat). Bone defects (3mm)was created in femur of each rat,one in the left side that left to heal spontaneously without any application,while the right side with application of a combination of 0.5 μl of BMP2 and 1μl of BDNF .Evaluation of histological changes includes bone cell counting and scoring of inflammation were estimated at different periods. Results: Histological examination of Bone healing with application of BMP2 & BDNF for normal and osteoporotic rat shows formation of woven bone and bone trabeculae that filled most of bone hole with minimal inflammatory response and with a significant differences value in comparism to inapplicable bone. Conclusion: The study concludes that application of the combination of BMP-2 & BDNF enhanced bone repair in osteoporotic rat.

Keywords

BMP-2, osteoporosis, bone defect, bone healing, BDNF, bone cells

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Noor Natik Raheem ¹, Mohammed Faris ², Athraa Y. Al-Hijazi ³, Ali I Alqurshi ⁴

Affiliations
1 Assistance Lecturer at College of Dentistry, Mustansiriya University, Baghdad, IQ
2 Assistance Lecturerat, Al-Mustaqbal University College/Dentistry Department, Babel, IQ
3 Professor, Al-Mustaqbal University College/Dentistry Department, Babel
4 Teaching Assistantat, Al-Mustaqbal University College/Dentistry Department, Babel, IQ

Abstract

Background: Brain-derived neurotrophic factor (BDNF), recognized to be involved in differentiation and proliferation of specialized cell such as endothelial cell and osteoblast cell. Aim of the Study: The study was designed to identify the effect of exogenous BDNF on development of jaw and on neural marker expression. Materials and Method: Twenty pregnant rats,ten injected (I.M) with normal saline,other ten injected (I.M) with 0.1 ml of BDNF that received three doses at intervals (0,7,14 day of gestation period).Embryos at 16^th and 18^th intra-uterine life were used for histological and immunohistochemical evaluation for the development of prenatal jaw. Results: Illustrate an enhancement of bone development during proceeding embryonic periods for both study groups .Moreover, the experimental group shows a high significant difference in the mean of bone cell count at the period 18^th day in comparisum to 16^th day IUL,and in comparisum to control at 18^th day IUL. Results show positive expression of neuronal marker by proliferating nerve cells in control and experimental groups with significant difference. Conclusion: The study suggest that BDNF contributes to promoting the differentiation of bone cells and enhancement of bone formation by increasing expression of neuron marker.

Keywords

Brain derived neurotrophic factor, bone, embryogenesis, osteogenic cell, bone stem cell, rat development.

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