Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Comparative Study of the Effect of Chronic Aluminium Chloride Administration on the Expression of Endothelial Nitric Oxide Synthase in Rat Brain


Affiliations
1 Minia University, Egypt
     

   Subscribe/Renew Journal


Background: Aluminium chloride (AlCl3 ) is a known potent environmental neurotoxic agent causing progressive neurodegenerative changes in the brain. Increasing evidence suggests that oxidative stressis strongly related to AlCl 3 neurotoxicity. Endothelial nitric oxide synthase (eNOS) is aniso form of the nitric oxide (NO) synthases family.

Aim of the Work:The current study was carried out to investigate the profile of the expression of eNOS in differentbrain regionsfollowing chronic aluminium chloride administration.

Materials and Method: Twelve adult male albinorats were divided equally into two groups. Group I was the untreated control, groups II was given Alcl 3 (100 mg/kg body weight) orally daily 42 days. At the end of the experiment, rats were killed by decapitation under anaesthesia. The brains were removed and processed for immunohistochemistry using antibody raised against eNOS.

Results:By comparison to the untreated control, Alcl3 treated rats showed significant (P<0.001) increase of eNOS expression in capillaries of cerebral cortex, hippocampus and cerebellum. In addition, there was significant (P<0.001) increase of eNOSexpression in hippocampus when compared to cortex and cerebellum.

Conclusion:These data provide further evidence that chronic exposure to aluminium chloride potentiate oxidative stress with subsequente NOS induction in different brain regions with more hippocampal affection.


Keywords

Aluminium Chloride, Neurodetoxicity, eNOS, Oxidative Stress, Brain, Hippocampus.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 96

PDF Views: 0




  • Comparative Study of the Effect of Chronic Aluminium Chloride Administration on the Expression of Endothelial Nitric Oxide Synthase in Rat Brain

Abstract Views: 96  |  PDF Views: 0

Authors

Sahar Ahmed Mokhemer
Minia University, Egypt
Nashwa Fathy Gamal El-Tahawy
Minia University, Egypt
Rehab Ahmed Rifaai
Minia University, Egypt
Entesar Ali Saber
Minia University, Egypt
Seham A. Abd El-Aleem
Minia University, Egypt

Abstract


Background: Aluminium chloride (AlCl3 ) is a known potent environmental neurotoxic agent causing progressive neurodegenerative changes in the brain. Increasing evidence suggests that oxidative stressis strongly related to AlCl 3 neurotoxicity. Endothelial nitric oxide synthase (eNOS) is aniso form of the nitric oxide (NO) synthases family.

Aim of the Work:The current study was carried out to investigate the profile of the expression of eNOS in differentbrain regionsfollowing chronic aluminium chloride administration.

Materials and Method: Twelve adult male albinorats were divided equally into two groups. Group I was the untreated control, groups II was given Alcl 3 (100 mg/kg body weight) orally daily 42 days. At the end of the experiment, rats were killed by decapitation under anaesthesia. The brains were removed and processed for immunohistochemistry using antibody raised against eNOS.

Results:By comparison to the untreated control, Alcl3 treated rats showed significant (P<0.001) increase of eNOS expression in capillaries of cerebral cortex, hippocampus and cerebellum. In addition, there was significant (P<0.001) increase of eNOSexpression in hippocampus when compared to cortex and cerebellum.

Conclusion:These data provide further evidence that chronic exposure to aluminium chloride potentiate oxidative stress with subsequente NOS induction in different brain regions with more hippocampal affection.


Keywords


Aluminium Chloride, Neurodetoxicity, eNOS, Oxidative Stress, Brain, Hippocampus.



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195077