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Modification of Ames Test According to Phenotype Features of Prototrophic Escherichia Coli


Affiliations
1 Tikrit University, College of Sciences, Department of Biology, Iraq
2 Department of Biology, University of Tikrit, Tikrit, Iraq
     

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Background:Iraq is an oil country in addition to an agricultural economie so the using of pesticide has raised very dramatically to cater the food demands of increasing population. Thus, a continuous examination of pesticides is required. Ames test is a standard method for determining the carcinogenic compounds by induce back mutations in auxotrophic strain of Salmonella typhimurium- histidine negative (his -) and biotin-negative (bio -), but in our country-Iraq, this bacterial strain was unavailable in most time. However, this study designed to guess the possibility of employment prototrophic Escherichia coli according to phenotype features instead of S. typhimurium.

Method: A prototrophic Escherichia coli was sensitive to amikacin, erythromycin, chloramphenicol, ciprofloxacin, tobramycin and trimethoprim. The strain was exposed to different concentration of Chlorpyrifos (CPF)as a mutation inducer. The potentiality of mutagenesisof CPF was detected according to phenotype featuresusing antibiotics susceptibility test (AST).

Result:Our result showed that the mutations were induced in some chromosomal genes of E. coli when exposed to CPF in high concentration 80-100%, while con. 10-70% were not. These genes encoded resistance to erythromycin, trimethoprim and tobramycin.

Conclusions:The modified Ames test can consider as a predictive tool for screening a potential carcinogenicity. The potential of genotoxic of the CPF has been detected.


Keywords

Mutagenic, Carcinogenic, Prototrophic Escherichia Coli, Chlorpyrifos.
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  • Modification of Ames Test According to Phenotype Features of Prototrophic Escherichia Coli

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Authors

Ibraheem Abdulrahman Eltaif
Tikrit University, College of Sciences, Department of Biology, Iraq
Dunia Kamal Salim
Department of Biology, University of Tikrit, Tikrit, Iraq
Sundus Jassim Muhammad
Department of Biology, University of Tikrit, Tikrit, Iraq

Abstract


Background:Iraq is an oil country in addition to an agricultural economie so the using of pesticide has raised very dramatically to cater the food demands of increasing population. Thus, a continuous examination of pesticides is required. Ames test is a standard method for determining the carcinogenic compounds by induce back mutations in auxotrophic strain of Salmonella typhimurium- histidine negative (his -) and biotin-negative (bio -), but in our country-Iraq, this bacterial strain was unavailable in most time. However, this study designed to guess the possibility of employment prototrophic Escherichia coli according to phenotype features instead of S. typhimurium.

Method: A prototrophic Escherichia coli was sensitive to amikacin, erythromycin, chloramphenicol, ciprofloxacin, tobramycin and trimethoprim. The strain was exposed to different concentration of Chlorpyrifos (CPF)as a mutation inducer. The potentiality of mutagenesisof CPF was detected according to phenotype featuresusing antibiotics susceptibility test (AST).

Result:Our result showed that the mutations were induced in some chromosomal genes of E. coli when exposed to CPF in high concentration 80-100%, while con. 10-70% were not. These genes encoded resistance to erythromycin, trimethoprim and tobramycin.

Conclusions:The modified Ames test can consider as a predictive tool for screening a potential carcinogenicity. The potential of genotoxic of the CPF has been detected.


Keywords


Mutagenic, Carcinogenic, Prototrophic Escherichia Coli, Chlorpyrifos.



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195060