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Cytotoxic Activity and Selectivity Index of Solanum Torvum Fruit on T47D Breast Cancer Cells


Affiliations
1 Faculty of Agriculture, Universitas Islam Kadiri, Indonesia
2 Faculty of Agriculture Technology Universitas Brawijaya Malang, Indonesia
     

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Solanum torvum has antioksidan. This study aimed to analyse cytotoxic activity and selectivity index of Solanum torvum on T47D breast cancer cells invitro. Identification of Solanum torvum fruit chemical compounds with LCMS containing Clorogenic acid, 4-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-feruloyquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,4 - O dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, putative dicaffeoylquinic lactone. Cytotoxic assays showed that the Solanum torvum Without Fermentation were IC50 (IGT) 1111.62μg/ mL, IC50 (MGT)1889.20μg/mL, IC50 (EGT) 1171.90μg/mL, IC50 (FEGT) 305.98 μg/mL. Cytotoxic assays showed that the treatment of fermentation was IC50 (IBT) 1760.36 μg/mL, IC50 (MBT) 1631.06 μg/mL, IC50 (EBT) of 1111.58 μg/mL, IC50 (FEBT) 39.17μg/mL, IC50 (FHBT) 85.58 μg/mL. Index of selectivity (IS)Solanum torvum (without fermentation) were IS (IGT) 5.23, IS (MGT) 0.61, IS (EGT) 1.06, IS (FEGT) 1.45. Index of selectivity of Solanum torvum (fermentation treatment) were IS (IBT) 2.41, IS (MBT) 0.78, IS (EBT) 0.18, IS (FEBT) 4.64, IS (FHBT) 1.67. The best results are treatment of ethyl acetate fraction of Solanum torvum(FEBT) has IC50 was 39.17 μg/mL and Index selectivity was 4.64, declared safe/not toxic to normal cells, thus potentially developed as T47D breast anticancer cells.

Keywords

Cytotoxic, T47D Breast Cells, Selectivity Index, Solanum torvum
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  • Cytotoxic Activity and Selectivity Index of Solanum Torvum Fruit on T47D Breast Cancer Cells

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Authors

Nunuk Helilusiatiningsih
Faculty of Agriculture, Universitas Islam Kadiri, Indonesia
Yunianta
Faculty of Agriculture Technology Universitas Brawijaya Malang, Indonesia
Harijono
Faculty of Agriculture Technology Universitas Brawijaya Malang, Indonesia
Simon Bambang Wijanarko
Faculty of Agriculture Technology Universitas Brawijaya Malang, Indonesia

Abstract


Solanum torvum has antioksidan. This study aimed to analyse cytotoxic activity and selectivity index of Solanum torvum on T47D breast cancer cells invitro. Identification of Solanum torvum fruit chemical compounds with LCMS containing Clorogenic acid, 4-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-feruloyquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,4 - O dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3-O-feruloylquinic acid, 5-O-feruloylquinic acid, putative dicaffeoylquinic lactone. Cytotoxic assays showed that the Solanum torvum Without Fermentation were IC50 (IGT) 1111.62μg/ mL, IC50 (MGT)1889.20μg/mL, IC50 (EGT) 1171.90μg/mL, IC50 (FEGT) 305.98 μg/mL. Cytotoxic assays showed that the treatment of fermentation was IC50 (IBT) 1760.36 μg/mL, IC50 (MBT) 1631.06 μg/mL, IC50 (EBT) of 1111.58 μg/mL, IC50 (FEBT) 39.17μg/mL, IC50 (FHBT) 85.58 μg/mL. Index of selectivity (IS)Solanum torvum (without fermentation) were IS (IGT) 5.23, IS (MGT) 0.61, IS (EGT) 1.06, IS (FEGT) 1.45. Index of selectivity of Solanum torvum (fermentation treatment) were IS (IBT) 2.41, IS (MBT) 0.78, IS (EBT) 0.18, IS (FEBT) 4.64, IS (FHBT) 1.67. The best results are treatment of ethyl acetate fraction of Solanum torvum(FEBT) has IC50 was 39.17 μg/mL and Index selectivity was 4.64, declared safe/not toxic to normal cells, thus potentially developed as T47D breast anticancer cells.

Keywords


Cytotoxic, T47D Breast Cells, Selectivity Index, Solanum torvum



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195053